AXL在胃癌中作为一种新的正调节因子的鉴定。

IF 4.4 2区生物学 Q2 CELL BIOLOGY

Cellular signalling Pub Date : 2024-12-19 DOI:10.1016/j.cellsig.2024.111573

Zhi Yang , Chuanfu Ren , Ziyun He , Banxin Luo , Xin Chen , En Xu , Wenxian Guan , Xuefeng Xia

{"title":"AXL在胃癌中作为一种新的正调节因子的鉴定。","authors":"Zhi Yang , Chuanfu Ren , Ziyun He , Banxin Luo , Xin Chen , En Xu , Wenxian Guan , Xuefeng Xia","doi":"10.1016/j.cellsig.2024.111573","DOIUrl":null,"url":null,"abstract":"<div><div>Lipid rafts are highly heterogeneous and dynamic microdomains involved in molecule trafficking and signaling transduction. This study investigates the role of lipid rafts in gastric cancer and their key regulators. Analyzing FFPE samples from 111 gastric cancer patients, we found that high lipid raft levels predict poor prognosis. Modulating these levels in gastric cancer cell lines significantly impacted cell proliferation, migration, and invasion. Weighted Gene Co-expression Network Analysis identified AXL as a hub gene associated with lipid rafts. AXL knockdown experiments revealed its interaction with Caveolin-1, a caveolae lipid raft protein, which regulates lipid raft levels and promotes AKT and ERK signaling, enhancing cancer development and metastasis. In vivo tumorigenesis assays and survival analyses further supported these findings. This study underscores the significance of lipid rafts in gastric cancer and identifies AXL as a novel regulator, offering new insights into the molecular mechanisms of cancer progression and suggesting potential therapeutic targets.</div></div>","PeriodicalId":9902,"journal":{"name":"Cellular signalling","volume":"127 ","pages":"Article 111573"},"PeriodicalIF":4.4000,"publicationDate":"2024-12-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Identification of AXL as a novel positive regulator of lipid raft in gastric cancer\",\"authors\":\"Zhi Yang , Chuanfu Ren , Ziyun He , Banxin Luo , Xin Chen , En Xu , Wenxian Guan , Xuefeng Xia\",\"doi\":\"10.1016/j.cellsig.2024.111573\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Lipid rafts are highly heterogeneous and dynamic microdomains involved in molecule trafficking and signaling transduction. This study investigates the role of lipid rafts in gastric cancer and their key regulators. Analyzing FFPE samples from 111 gastric cancer patients, we found that high lipid raft levels predict poor prognosis. Modulating these levels in gastric cancer cell lines significantly impacted cell proliferation, migration, and invasion. Weighted Gene Co-expression Network Analysis identified AXL as a hub gene associated with lipid rafts. AXL knockdown experiments revealed its interaction with Caveolin-1, a caveolae lipid raft protein, which regulates lipid raft levels and promotes AKT and ERK signaling, enhancing cancer development and metastasis. In vivo tumorigenesis assays and survival analyses further supported these findings. This study underscores the significance of lipid rafts in gastric cancer and identifies AXL as a novel regulator, offering new insights into the molecular mechanisms of cancer progression and suggesting potential therapeutic targets.</div></div>\",\"PeriodicalId\":9902,\"journal\":{\"name\":\"Cellular signalling\",\"volume\":\"127 \",\"pages\":\"Article 111573\"},\"PeriodicalIF\":4.4000,\"publicationDate\":\"2024-12-19\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Cellular signalling\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0898656824005497\",\"RegionNum\":2,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Cellular signalling","FirstCategoryId":"99","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0898656824005497","RegionNum":2,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CELL BIOLOGY","Score":null,"Total":0}

引用次数: 0

摘要

脂筏是高度异质性和动态的微结构域，参与分子运输和信号转导。本研究探讨脂筏在胃癌中的作用及其关键调控因子。通过分析111例胃癌患者的FFPE样本，我们发现高脂筏水平预示着不良预后。在胃癌细胞系中调节这些水平可以显著影响细胞的增殖、迁移和侵袭。加权基因共表达网络分析发现AXL是与脂筏相关的枢纽基因。AXL敲低实验发现其与小泡脂筏蛋白Caveolin-1相互作用，调节脂筏水平，促进AKT和ERK信号传导，促进肿瘤的发生和转移。体内肿瘤发生试验和生存分析进一步支持了这些发现。本研究强调了脂筏在胃癌中的重要性，并确定了AXL作为一种新的调节因子，为癌症进展的分子机制提供了新的见解，并提出了潜在的治疗靶点。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Identification of AXL as a novel positive regulator of lipid raft in gastric cancer

查看原文本刊更多论文

Identification of AXL as a novel positive regulator of lipid raft in gastric cancer

Lipid rafts are highly heterogeneous and dynamic microdomains involved in molecule trafficking and signaling transduction. This study investigates the role of lipid rafts in gastric cancer and their key regulators. Analyzing FFPE samples from 111 gastric cancer patients, we found that high lipid raft levels predict poor prognosis. Modulating these levels in gastric cancer cell lines significantly impacted cell proliferation, migration, and invasion. Weighted Gene Co-expression Network Analysis identified AXL as a hub gene associated with lipid rafts. AXL knockdown experiments revealed its interaction with Caveolin-1, a caveolae lipid raft protein, which regulates lipid raft levels and promotes AKT and ERK signaling, enhancing cancer development and metastasis. In vivo tumorigenesis assays and survival analyses further supported these findings. This study underscores the significance of lipid rafts in gastric cancer and identifies AXL as a novel regulator, offering new insights into the molecular mechanisms of cancer progression and suggesting potential therapeutic targets.

求助全文

通过发布文献求助，成功后即可免费获取论文全文。去求助

来源期刊

Cellular signalling 生物-细胞生物学

CiteScore

8.40

自引率

0.00%

发文量

250

审稿时长

27 days

期刊介绍： Cellular Signalling publishes original research describing fundamental and clinical findings on the mechanisms, actions and structural components of cellular signalling systems in vitro and in vivo. Cellular Signalling aims at full length research papers defining signalling systems ranging from microorganisms to cells, tissues and higher organisms.