{"title":"S100A11 is a potential prognostic biomarker and correlated with tumor immunosuppressive microenvironment in glioma.","authors":"Jikang Fan, Xuya Wang, Xuejun Yang","doi":"10.1097/MD.0000000000040701","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>This study investigates the role of S100A11 as a potential biomarker for glioma-associated macrophages (GAMs) and its correlation with GAMs infiltration in glioblastoma multiforme, aiming to better understand the immune microenvironment of glioma.</p><p><strong>Methods: </strong>We conducted a comprehensive study using various techniques and approaches. First, we examined the expression of S100A11 on GAMs through Western blot, immunohistochemistry, and immunofluorescence analyses. Additionally, we utilized single-cell sequencing data and immune infiltration analysis to establish the relationship between S100A11 and GAMs infiltration in glioma.</p><p><strong>Results: </strong>Our findings revealed that S100A11 was highly expressed on GAMs, as validated by Western blot, immunohistochemistry, and immunofluorescence. Moreover, S100A11 exhibited a strong correlation with GAMs infiltration, as evidenced by single-cell data and immune infiltration analysis. These results highlight the significant association of S100A11 with the immune microenvironment surrounding glioma.</p><p><strong>Conclusion: </strong>S100A11 emerges as a promising candidate for regulating glioma immunosuppression and may serve as a potential marker for GAMs. This study sheds light on the crucial role of S100A11 in the crosstalk between GAMs and glioma cells, contributing to our understanding of glioma progression within the tumor microenvironment.</p>","PeriodicalId":18549,"journal":{"name":"Medicine","volume":"103 51","pages":"e40701"},"PeriodicalIF":1.3000,"publicationDate":"2024-12-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11666167/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Medicine","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1097/MD.0000000000040701","RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"MEDICINE, GENERAL & INTERNAL","Score":null,"Total":0}
引用次数: 0
摘要
研究背景本研究探讨了S100A11作为胶质瘤相关巨噬细胞（GAMs）潜在生物标志物的作用及其与多形性胶质母细胞瘤中GAMs浸润的相关性，旨在更好地了解胶质瘤的免疫微环境：我们采用多种技术和方法进行了一项综合研究。首先，我们通过 Western 印迹、免疫组化和免疫荧光分析检测了 S100A11 在胶质母细胞瘤上的表达。此外，我们还利用单细胞测序数据和免疫浸润分析来确定 S100A11 与胶质瘤中 GAMs 浸润之间的关系：结果：我们的研究结果表明，S100A11在GAMs上高表达，Western印迹、免疫组化和免疫荧光均验证了这一点。此外，单细胞数据和免疫浸润分析表明，S100A11与GAMs浸润密切相关。这些结果凸显了S100A11与胶质瘤周围免疫微环境的重要关联：结论：S100A11有望成为调节胶质瘤免疫抑制的候选者，并可作为GAMs的潜在标记物。这项研究揭示了S100A11在GAMs和胶质瘤细胞之间的串扰中的关键作用，有助于我们了解胶质瘤在肿瘤微环境中的进展。
S100A11 is a potential prognostic biomarker and correlated with tumor immunosuppressive microenvironment in glioma.
Background: This study investigates the role of S100A11 as a potential biomarker for glioma-associated macrophages (GAMs) and its correlation with GAMs infiltration in glioblastoma multiforme, aiming to better understand the immune microenvironment of glioma.
Methods: We conducted a comprehensive study using various techniques and approaches. First, we examined the expression of S100A11 on GAMs through Western blot, immunohistochemistry, and immunofluorescence analyses. Additionally, we utilized single-cell sequencing data and immune infiltration analysis to establish the relationship between S100A11 and GAMs infiltration in glioma.
Results: Our findings revealed that S100A11 was highly expressed on GAMs, as validated by Western blot, immunohistochemistry, and immunofluorescence. Moreover, S100A11 exhibited a strong correlation with GAMs infiltration, as evidenced by single-cell data and immune infiltration analysis. These results highlight the significant association of S100A11 with the immune microenvironment surrounding glioma.
Conclusion: S100A11 emerges as a promising candidate for regulating glioma immunosuppression and may serve as a potential marker for GAMs. This study sheds light on the crucial role of S100A11 in the crosstalk between GAMs and glioma cells, contributing to our understanding of glioma progression within the tumor microenvironment.
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