IF 2.4 4区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Sanaz Salek, Elham Moazamian, Afshin Mohammadi Bardbori, Seyedeh Azra Shamsdin
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引用次数: 0

摘要

结肠直肠癌(CRC)是死亡率最高的癌症类型,由于其耐药性和副作用,因此需要5-氟尿嘧啶(5-FU)的辅助药物。益生菌产生的代谢物对 CRC 具有良好的抗癌作用。本研究评估了三种从骆驼奶中分离出来的潜在益生菌株--螺旋乳杆菌、加里纳氏乳杆菌和植物乳杆菌,以及标准益生菌株--鼠李糖乳杆菌 GG(LGG)的细胞提取物对人类结肠癌细胞系(HT-29)和正常 HEK293 细胞系单独或与 5-FU 联用的抗癌效果。这项研究从骆驼奶中分离出菌株,并比较了它们与 LGG 的益生特性。分别使用 MTT 试验、吖啶橙/溴化乙锭(AO/EB)染色和流式细胞仪技术评估了细胞活力、细胞凋亡和 Th17 细胞因子的产生。乳酸菌菌株的细胞提取物与 5-FU 结合使用能有效降低 HT-29 细胞的存活率并增加细胞凋亡。然而,与 5-FU 结合使用的乳酸菌菌株细胞提取物控制了 5-FU 对 HEK-293 细胞活力的细胞毒性影响,并减少了细胞凋亡。各菌株之间没有发现明显差异。此外,与 5-FU 结合使用的菌株的细胞提取物提高了细胞因子 IFN-γ、TNF-α 和 IL-17A 的水平,所有这些因子都有助于提高抗肿瘤免疫力。研究菌株的表现与标准益生菌菌株(LGG)相似。研究结果表明,从乳酸菌菌株中提取的细胞萃取物可以作为一种很有前景的辅助抗癌疗法。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Anticancer effect of a combinatorial treatment of 5-fluorouracil and cell extract of some probiotic lactobacilli strains isolated from camel milk on colorectal cancer cells.

Colorectal cancer (CRC) has the highest mortality rate among cancer types, emphasizing the need for auxiliaries to 5-fluorouracil (5-FU) due to resistance and side effects. Metabolites produced by probiotic bacteria exhibit promising anticancer properties against CRC. In the current study, the anticancer effects of cell extract of three potential probiotic lactobacilli strains isolated from camel milk, Lactobacillus helveticus, Lactobacillus gallinarum, and Lactiplantibacillus plantarum, as well as that of the standard probiotic strain Lacticaseibacillus rhamnosus GG (LGG), on the human colon cancer cell line (HT-29) and the normal HEK293 cell line separately or in combination with 5-FU, were evaluated. This study isolated strains from camel milk and compared their probiotic properties to those of LGG. The cell viability, cell apoptosis, and Th17 cytokine production were assessed using the MTT assay, acridine orange/ethidium bromide (AO/EB) staining, and flow cytometry techniques, respectively. The cell extracts of lactobacilli strains combined with 5-FU reduced HT-29 cell viability effectively and increased cell apoptosis. Nevertheless, the cell extracts of lactobacilli strains combined with 5-FU controlled the cytotoxic impact of 5-FU on HEK-293 cell viability and reduced cell apoptosis. No significant differences were observed among the strains. Moreover, the cell extracts from the strains combined with 5-FU increased the levels of cytokines IFN-γ, TNF-α, and IL-17A, all of which contribute to immunity against tumors. The performance of the studied strains was similar to that of the standard probiotic strain (LGG). The investigation revealed that cell extracts from lactobacilli strains may serve as a promising complementary anticancer treatment.

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来源期刊
Folia microbiologica
Folia microbiologica 工程技术-生物工程与应用微生物
CiteScore
5.80
自引率
0.00%
发文量
82
审稿时长
6-12 weeks
期刊介绍: Unlike journals which specialize ever more narrowly, Folia Microbiologica (FM) takes an open approach that spans general, soil, medical and industrial microbiology, plus some branches of immunology. This English-language journal publishes original papers, reviews and mini-reviews, short communications and book reviews. The coverage includes cutting-edge methods and promising new topics, as well as studies using established methods that exhibit promise in practical applications such as medicine, animal husbandry and more. The coverage of FM is expanding beyond Central and Eastern Europe, with a growing proportion of its contents contributed by international authors.
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