{"title":"具有过氧化物酶样活性的 Fe/Pt 掺杂碳纳米粒子用于尿酸的床旁检测","authors":"Xia Tong, Guozhu Li, Qianyu Guo, Jie Hu, Baiyan Zhang, Sumiao Liu, Jing Guo, Liyun Zhang","doi":"10.1007/s00604-024-06861-9","DOIUrl":null,"url":null,"abstract":"<div><p> A pasting-3D microfluidic paper-based analytical device (P-3D μPAD) was developed. It enabled an efficient cascade reaction between urate oxidase (UOX) and Fe/Pt-doped carbon nanoparticles (Fe/Pt-CNPs) for visual colorimetric detection of uric acid (UA). The novel Fe/Pt-CNP nanozyme performed high peroxidase-like activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) and H<sub>2</sub>O<sub>2</sub> with Michaelis − Menten constants (K<sub>m</sub>) of 0.97 and 2.30 mM, respectively. The UOX-Fe/Pt-CNP system was incorporated into P-3D μPAD: the 1st layer was UOX hydrolysis reaction under alkaline pH, and the 2nd layer was Fe/Pt-CNPs catalyzing H<sub>2</sub>O<sub>2</sub> to oxidize TMB at acidic pH. The separated two layers allowed cascade reaction under different working pH without sacrificing the activity of UOX or Fe/Pt-CNPs. The images were captured and analyzed by the camera and “Color Recognition” application using a smartphone. The linear range of P-3D μPAD for UA was 25–1000 μM with a limit of detection of 10 μM which met the requirements for clinical applications. The high accuracy of P-3D μPAD for UA detection in invasive (blood) and noninvasive (saliva) samples has been confirmed by the biochemical analyzer. This work offers a sensitive, flexible, affordable, and disposable tool for on-site UA level monitoring and provides new insight into natural enzyme and nanozyme tandem systems for biosensing.</p></div>","PeriodicalId":705,"journal":{"name":"Microchimica Acta","volume":"192 1","pages":""},"PeriodicalIF":5.3000,"publicationDate":"2024-12-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Fe/Pt-doped carbon nanoparticles with peroxidase-like activity for point-of-care determination of uric acid\",\"authors\":\"Xia Tong, Guozhu Li, Qianyu Guo, Jie Hu, Baiyan Zhang, Sumiao Liu, Jing Guo, Liyun Zhang\",\"doi\":\"10.1007/s00604-024-06861-9\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p> A pasting-3D microfluidic paper-based analytical device (P-3D μPAD) was developed. It enabled an efficient cascade reaction between urate oxidase (UOX) and Fe/Pt-doped carbon nanoparticles (Fe/Pt-CNPs) for visual colorimetric detection of uric acid (UA). The novel Fe/Pt-CNP nanozyme performed high peroxidase-like activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) and H<sub>2</sub>O<sub>2</sub> with Michaelis − Menten constants (K<sub>m</sub>) of 0.97 and 2.30 mM, respectively. The UOX-Fe/Pt-CNP system was incorporated into P-3D μPAD: the 1st layer was UOX hydrolysis reaction under alkaline pH, and the 2nd layer was Fe/Pt-CNPs catalyzing H<sub>2</sub>O<sub>2</sub> to oxidize TMB at acidic pH. The separated two layers allowed cascade reaction under different working pH without sacrificing the activity of UOX or Fe/Pt-CNPs. The images were captured and analyzed by the camera and “Color Recognition” application using a smartphone. The linear range of P-3D μPAD for UA was 25–1000 μM with a limit of detection of 10 μM which met the requirements for clinical applications. The high accuracy of P-3D μPAD for UA detection in invasive (blood) and noninvasive (saliva) samples has been confirmed by the biochemical analyzer. This work offers a sensitive, flexible, affordable, and disposable tool for on-site UA level monitoring and provides new insight into natural enzyme and nanozyme tandem systems for biosensing.</p></div>\",\"PeriodicalId\":705,\"journal\":{\"name\":\"Microchimica Acta\",\"volume\":\"192 1\",\"pages\":\"\"},\"PeriodicalIF\":5.3000,\"publicationDate\":\"2024-12-21\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Microchimica Acta\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s00604-024-06861-9\",\"RegionNum\":2,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Microchimica Acta","FirstCategoryId":"92","ListUrlMain":"https://link.springer.com/article/10.1007/s00604-024-06861-9","RegionNum":2,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
Fe/Pt-doped carbon nanoparticles with peroxidase-like activity for point-of-care determination of uric acid
A pasting-3D microfluidic paper-based analytical device (P-3D μPAD) was developed. It enabled an efficient cascade reaction between urate oxidase (UOX) and Fe/Pt-doped carbon nanoparticles (Fe/Pt-CNPs) for visual colorimetric detection of uric acid (UA). The novel Fe/Pt-CNP nanozyme performed high peroxidase-like activity toward 3,3′,5,5′-tetramethylbenzidine (TMB) and H2O2 with Michaelis − Menten constants (Km) of 0.97 and 2.30 mM, respectively. The UOX-Fe/Pt-CNP system was incorporated into P-3D μPAD: the 1st layer was UOX hydrolysis reaction under alkaline pH, and the 2nd layer was Fe/Pt-CNPs catalyzing H2O2 to oxidize TMB at acidic pH. The separated two layers allowed cascade reaction under different working pH without sacrificing the activity of UOX or Fe/Pt-CNPs. The images were captured and analyzed by the camera and “Color Recognition” application using a smartphone. The linear range of P-3D μPAD for UA was 25–1000 μM with a limit of detection of 10 μM which met the requirements for clinical applications. The high accuracy of P-3D μPAD for UA detection in invasive (blood) and noninvasive (saliva) samples has been confirmed by the biochemical analyzer. This work offers a sensitive, flexible, affordable, and disposable tool for on-site UA level monitoring and provides new insight into natural enzyme and nanozyme tandem systems for biosensing.
期刊介绍：
As a peer-reviewed journal for analytical sciences and technologies on the micro- and nanoscale, Microchimica Acta has established itself as a premier forum for truly novel approaches in chemical and biochemical analysis. Coverage includes methods and devices that provide expedient solutions to the most contemporary demands in this area. Examples are point-of-care technologies, wearable (bio)sensors, in-vivo-monitoring, micro/nanomotors and materials based on synthetic biology as well as biomedical imaging and targeting.
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