用于危险表征的化学混合物的生物活性分析

IF 10.8 1区 环境科学与生态学 Q1 ENGINEERING, ENVIRONMENTAL
Xiaojing Li, Jiarui Zhou, Yaohui Bai, Meng Qiao, Wei Xiong, Tobias Schulze, Martin Krauss, Timothy D. Williams, Ben Brown, Luisa Orsini, Liang-Hong Guo, John K. Colbourne
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引用次数: 0

摘要

为保护人类健康和环境而对化学品毒性进行的评估和管制是一次对一种化学品进行的,很少按与环境有关的浓度进行。然而,化学物质在环境中以混合物的形式存在,它们的毒性在很大程度上是未知的。了解混合物中的化学物质所造成的危害对于执行保护措施至关重要。在这里,我们展示了使用哨兵和生态毒理学模型物种水蚤对环境水样进行生物活性分析的应用,以揭示暴露于现实世界混合物所引起的生物分子反应。我们将一株水蚤暴露在潮白河的30个采样水中,并测量了基因表达响应谱。利用多块相关分析,我们在30个水样中鉴定出的化学混合物与这些化学混合物诱导的基因表达模式之间建立了相关性。我们确定了80种代谢途径,这些途径被无机离子、重金属、多环芳烃、工业化学品和一组杀菌剂、杀虫剂和药理活性物质的混合物推定激活。我们的数据驱动方法发现了已知的生物活性特征和先前描述的作用模式,以及与未发现的潜在危害相关的新途径。本研究证明了降低现实世界混合物毒性复杂性的可行性,以表征基于哨兵物种水蚤基因表达监测的特定数量的化学成分的生物分子效应。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Bioactivity Profiling of Chemical Mixtures for Hazard Characterization

Bioactivity Profiling of Chemical Mixtures for Hazard Characterization
The assessment and regulation of chemical toxicity to protect human health and the environment are done one chemical at a time and seldom at environmentally relevant concentrations. However, chemicals are found in the environment as mixtures, and their toxicity is largely unknown. Understanding the hazard posed by chemicals within the mixture is critical to enforce protective measures. Here, we demonstrate the application of bioactivity profiling of environmental water samples using the sentinel and ecotoxicology model species Daphnia to reveal the biomolecular response induced by exposure to real-world mixtures. We exposed a Daphnia strain to 30 sampled waters of the Chaobai River and measured the gene expression response profiles. Using a multiblock correlation analysis, we establish correlations between chemical mixtures identified in 30 water samples with gene expression patterns induced by these chemical mixtures. We identified 80 metabolic pathways putatively activated by mixtures of inorganic ions, heavy metals, polycyclic aromatic hydrocarbons, industrial chemicals, and a set of biocides, pesticides, and pharmacologically active substances. Our data-driven approach discovered both known bioactivity signatures with previously described modes of action and new pathways linked to undiscovered potential hazards. This study demonstrates the feasibility of reducing the complexity of real-world mixture toxicity to characterize the biomolecular effects of a defined number of chemical components based on gene expression monitoring of the sentinel species Daphnia.
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来源期刊
环境科学与技术
环境科学与技术 环境科学-工程:环境
CiteScore
17.50
自引率
9.60%
发文量
12359
审稿时长
2.8 months
期刊介绍: Environmental Science & Technology (ES&T) is a co-sponsored academic and technical magazine by the Hubei Provincial Environmental Protection Bureau and the Hubei Provincial Academy of Environmental Sciences. Environmental Science & Technology (ES&T) holds the status of Chinese core journals, scientific papers source journals of China, Chinese Science Citation Database source journals, and Chinese Academic Journal Comprehensive Evaluation Database source journals. This publication focuses on the academic field of environmental protection, featuring articles related to environmental protection and technical advancements.
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