高寒提取物对实验性肺纤维化的影响：Sirt1和Nrf2抗氧化途径的相关作用。

Q3 Medicine

Morphologie Pub Date : 2024-12-17 DOI:10.1016/j.morpho.2024.100940

Marwa A.S. Al-Gholam , Huda I. Abd-Elhafiz , Sara G. Tayel

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引用次数: 0

摘要

背景：肺纤维化（PF）是常见的COVID-19后遗症。它是一种以呼吸衰竭和死亡为特征的进行性疾病。高寒根茎（AO）的特性使其成为一种高效的抗氧化剂、抗炎剂和抗纤维化剂。本研究评估了AO对博莱霉素诱导大鼠PF的保护作用，并探讨了其机制。材料与方法：采用博莱霉素（5mg/kg，气管内灌胃）诱导白化大鼠PF，博莱霉素灌胃后给予AO提取物（200mg/kg/d，口服）28d。大鼠安乐死后进行肺组织生化、定量实时聚合酶链反应（qPCR）及组织病理学检查。结果：研究结果显示，博莱霉素显著提高了组织中丙二醛、肿瘤坏死因子- α、白细胞介素-6、沉默信息调节因子1 （Sirt1）和核因子红细胞2相关因子2 (Nrf2) mRNA水平。此外，博莱霉素灌注大鼠肺总抗氧化能力水平下降。然而，AO提取物显著降低了苏木精和伊红、马松三色染色切片、诱导型一氧化氮合酶和α-平滑肌肌动蛋白、转化生长因子β 1免疫表达的组织病理学损伤。结论：大连翘根茎提取物可能具有抗氧化、抗炎和抗纤维化的作用，对博莱霉素诱导的PF具有保护作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Effect of Alpinia officinarum Rhizome extract on experimentally induced lung fibrosis: The pertinent role of Sirt1 and Nrf2 antioxidant pathways

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Effect of Alpinia officinarum Rhizome extract on experimentally induced lung fibrosis: The pertinent role of Sirt1 and Nrf2 antioxidant pathways

Background

Pulmonary fibrosis (PF) is a frequently reported COVID-19 sequela. It is a progressive disorder characterized by respiratory failure and death. The properties of Alpinia officinarum Rhizomes (AO) make it a highly potent antioxidant, anti-inflammatory, and antifibrotic agent. This study has evaluated AO's protective effects on bleomycin-induced PF in rats and investigated the underlying mechanisms.

Material and methods

Bleomycin (5 mg/kg, intratracheally) was used to induce PF in albino rats, and then, AO extract (200 mg/kg/daily, orally) was administrated for 28 days post-bleomycin-instillation. After euthanizing the rats, the biochemical, quantitative real-time polymerase chain reaction (qPCR) and histopathological examination of lung tissue were determined.

Results

Findings have revealed that bleomycin significantly increased the tissue level of malondialdehyde, tumor necrosis factor-alpha, and interleukin-6, Silent information regulator 1 (Sirt1), and nuclear factor erythroid 2-related factor 2 (Nrf2) mRNA levels. Furthermore, the total antioxidant capacity level decreased in the lungs of bleomycin-instilled rats. However, AO extract significantly decreased histopathological injuries in hematoxylin & eosin, Masson's trichrome-stained sections, inducible nitric oxide synthase and α-smooth muscle actin, transforming growth factor beta 1 immunoexpression.

Conclusion

Alpinia officinarum Rhizomes extract appears to protect against bleomycin-induced PF, possibly due to its antioxidant, anti-inflammatory, and antifibrotic properties.

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来源期刊

Morphologie Medicine-Anatomy

CiteScore

2.30

自引率

0.00%

发文量

150

审稿时长

25 days

期刊介绍： Morphologie est une revue universitaire avec une ouverture médicale qui sa adresse aux enseignants, aux étudiants, aux chercheurs et aux cliniciens en anatomie et en morphologie. Vous y trouverez les développements les plus actuels de votre spécialité, en France comme a international. Le objectif de Morphologie est d?offrir des lectures privilégiées sous forme de revues générales, d?articles originaux, de mises au point didactiques et de revues de la littérature, qui permettront notamment aux enseignants de optimiser leurs cours et aux spécialistes d?enrichir leurs connaissances.