PVA/阳离子淀粉/氧化锌喷雾干燥微胶囊化巨型芽孢杆菌促进大豆生长和锌的利用

IF 2.3 Q1 AGRICULTURE, MULTIDISCIPLINARY
Ludimila Araújo Lodi, Roger Borges, Marina Momesso Lopes, Vanessa Araújo Graciano, Ricardo Bortoletto-Santos, Hernane S. Barud, Christiane Abreu de Oliveira-Paiva, Caue Ribeiro and Cristiane Sanchez Farinas*, 
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引用次数: 0

摘要

锌(Zn)是植物发育所必需的,缺乏锌会降低农业生产力。营养物增溶微生物为提高植物锌的有效性提供了一个很有前途的解决方案。然而,直接应用这些微生物在现场提出了挑战,如细胞活力损失。本研究以聚乙烯醇(PVA)、阳离子淀粉(CS)和氧化锌(ZnO)为原料,采用喷雾干燥的方法对巨型芽孢杆菌进行微胶囊化。实验结果表明,白僵菌能有效地溶解氧化锌。PVACS-ZnO基质为巨芽孢杆菌的生长发育提供了良好的环境,释放的细胞数量超过了初始接种(10 log10 CFU/g)。此外,它可以保护细胞免受恶劣的野外条件,在加热(50°C/48小时)、紫外线照射(180分钟后95%)和杀菌剂/杀虫剂暴露(2小时后99%)后保持细菌活力,不像自由细菌。加速保质期测试表明PVACS-ZnO微球的稳定性延长,与游离细菌(6个月)相比,其预估保质期(14个月)延长了一倍。在温室试验中,该配方增加了大豆植株的地上和根系生物量,促进了磷和锌的吸收。这些发现表明,PVASC-ZnO配方为包裹微生物和提高锌的可用性提供了一种有前途的策略,从而产生一种有效且环保的生物肥料产品。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Spray-Drying Microencapsulation of Bacillus megaterium in PVA/Cationic Starch/Zinc Oxide for Promoting Growth and Zinc Availability in Soybean Plants

Zinc (Zn) is essential for plant development and its deficiency can reduce agricultural productivity. Nutrient-solubilizing microorganisms offer a promising solution to enhance the zinc availability for plants. However, directly applying these microorganisms in the field presents challenges such as cell viability loss. Here, we developed a formulation using poly(vinyl alcohol) (PVA), cationic starch (CS), and zinc oxide (ZnO) for microencapsulating Bacillus megaterium via spray drying. Our results showed that B. megaterium effectively solubilizes zinc oxide. The PVACS-ZnO matrix provided a favorable environment for the growth and development of B. megaterium, releasing cells in quantities exceeding initial inoculation (10 log10 CFU/g). Additionally, it protected the cells against adverse field conditions, maintaining bacterial viability after heat (50 °C/48 h), UV light (95% after 180 min), and fungicide/insecticide exposure (99% after 2 h), unlike free bacteria. Accelerated shelf life tests indicated prolonged stability of PVACS-ZnO microspheres, with double the estimated shelf life (14 months) compared to free bacteria (6 months). In greenhouse experiments, the formulation increased aerial and root biomass of soybean plants, and enhanced phosphorus and zinc absorption. These findings indicate that PVASC-ZnO formulations offer a promising strategy for encapsulating microorganisms and enhancing zinc availability, resulting in an effective and environmentally friendly biofertilizer product.

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CiteScore
2.80
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