IF 2.4 3区 物理与天体物理 Q1 Mathematics
Shaoying Zhu, Rui He, Rongjing Zhang, Junhua Yuan
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引用次数: 0

摘要

细菌鞭毛运动具有趋化和机械敏感性。它对细胞内趋化反应调节剂 CheY-P 的浓度和外部负载条件都很敏感。马达的剂量反应曲线表示马达顺时针旋转的概率(CW 偏移)与 CheY-P 浓度的函数关系,是其化学敏感性的特征。然而,剂量反应曲线如何取决于负载条件仍不清楚。在这里,我们测量了马达在各种负载条件下的剂量反应曲线。令人惊讶的是,我们发现在低 CW 偏置条件下,剂量反应曲线随负荷的变化极小,但在高 CW 偏置条件下,当负荷增加时,剂量反应曲线向左移动,对 CheY-P 浓度的敏感性更高。这一观察结果与之前的模型预测相矛盾,之前的模型预测包含了定转子相互作用对电机开关的影响。通过对鞭毛开关的化学和机械耦合敏感性建立一个 Ising 型模型,我们揭示了机械敏感性剂量反应的基本机制是马达开关复合体的适应性重塑与定子-转子相互作用的非平衡效应之间的协同作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mechanosensitive dose response of the bacterial flagellar motor.

The bacterial flagellar motor is both chemo- and mechanosensitive. It is sensitive to the intracellular concentration of the chemotaxis response regulator CheY-P and to external load conditions. The motor's dose-response curve, which represents the probability of the motor rotating clockwise (CW bias) as a function of CheY-P concentration, characterizes its chemical sensitivity. However, it remains unclear how this dose-response curve depends on the load conditions. Here, we measured the dose-response curves of the motor under various load conditions. Surprisingly, we found that the dose-response curve exhibited minimal changes with load at low CW biases, but shifted leftward with higher sensitivity to CheY-P concentration at high CW biases when the load increased. This observation contradicts previous model predictions that incorporated the effect of stator-rotor interaction on motor switching. Through the development of an Ising-type model for the coupled chemo- and mechanosensitivity of the flagellar switch, we revealed that the mechanism underlying the mechanosensitive dose response is the synergistic interplay between the adaptive remodeling of the motor switch complex and the nonequilibrium effect of the stator-rotor interaction.

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来源期刊
Physical review. E
Physical review. E 物理-物理:流体与等离子体
CiteScore
4.60
自引率
16.70%
发文量
0
审稿时长
3.3 months
期刊介绍: Physical Review E (PRE), broad and interdisciplinary in scope, focuses on collective phenomena of many-body systems, with statistical physics and nonlinear dynamics as the central themes of the journal. Physical Review E publishes recent developments in biological and soft matter physics including granular materials, colloids, complex fluids, liquid crystals, and polymers. The journal covers fluid dynamics and plasma physics and includes sections on computational and interdisciplinary physics, for example, complex networks.
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