{"title":"长鞘单胞菌、软体鞘单胞菌和金色鞘单胞菌：土壤中分离的三种鞘单胞菌新种。","authors":"Eo Jin Kim, Soo Hyun Maeng, Myung Kyum Kim","doi":"10.1099/ijsem.0.006572","DOIUrl":null,"url":null,"abstract":"<p><p>Three Gram-negative, aerobic and non-motile bacterial strains, BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup>, were isolated from soil samples in Gwangju-si and Gangneung-si, the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequence showed that strains BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup> clustered to a distinct clade within the family <i>Sphingomonadaceae</i> (order <i>Sphingomonadales</i>, class <i>Alphaproteobacteria</i>). The strains exhibited the highest genetic similarity with representatives of the genus <i>Sphingomonas</i>; moreover, strains BT552<sup>T</sup> and BT553<sup>T</sup> tightly clustered with <i>Sphingomonas melonis</i> DAPP-PG 224<sup>T</sup> (98.2 and 98.1 %) and <i>Sphingomonas aquatilis</i> JSS-7<sup>T</sup> (98.1 and 98.0 %), while strain KR1UV-12<sup>T</sup> clustered with <i>S. melonis</i> DAPP-PG 224<sup>T</sup> (97.9%) and <i>Sphingomonas rubra</i> BH3<sup>T</sup> (97.8%), respectively. The major cellular fatty acids of all three strains were summed feature 8 (C<sub>18:1</sub> ω7c/C<sub>18:1</sub> ω6c), comprising 44.7, 46.4 and 48.5%. Additionally, their respiratory quinone is Q-10, and polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids, sphingolipid and phosphatidylcholine. They all grow well at an optimum temperature of 25 °C, at pH 7. The draft genomes of strains BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup> measures 4 035 561 bp, 3 941 714 bp and 3 418 792 bp, respectively, comprising 3 804 3648 and 3236 coding sequences and 50, 48 and 45 RNA genes. The average nucleotide identity analysis and digital DNA-DNA hybridization values between BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup> and closely related <i>Sphingomonas</i> species range from 72.7 to 80.2% and 19.4 to 24.3%, respectively. Based on phenotypic, genotypic and chemotaxonomic data, these three strains BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup> represent three novel bacterial species within the genus <i>Sphingomonas</i> for which the names <i>Sphingomonas longa</i> sp. nov. (type strain BT552<sup>T</sup>= KCTC 82094<sup>T</sup> =NBRC 114993<sup>T</sup>), <i>Sphingomonas mollis</i> sp. nov. (type strain BT553<sup>T</sup> =KCTC 82095<sup>T</sup> =NBRC 114994<sup>T</sup>) and <i>Sphingomonas aurea</i> sp. nov. (type strain KR1UV-12<sup>T</sup> = KCTC 92959<sup>T</sup> = TBRC 18506<sup>T</sup>) are proposed.</p>","PeriodicalId":14390,"journal":{"name":"International journal of systematic and evolutionary microbiology","volume":"74 12","pages":""},"PeriodicalIF":2.0000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"<i>Sphingomonas longa</i> sp. nov., <i>Sphingomonas mollis</i> sp. nov. and <i>Sphingomonas aurea</i> sp. nov.: three novel <i>Sphingomonas</i> species isolated from soil.\",\"authors\":\"Eo Jin Kim, Soo Hyun Maeng, Myung Kyum Kim\",\"doi\":\"10.1099/ijsem.0.006572\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Three Gram-negative, aerobic and non-motile bacterial strains, BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup>, were isolated from soil samples in Gwangju-si and Gangneung-si, the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequence showed that strains BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup> clustered to a distinct clade within the family <i>Sphingomonadaceae</i> (order <i>Sphingomonadales</i>, class <i>Alphaproteobacteria</i>). The strains exhibited the highest genetic similarity with representatives of the genus <i>Sphingomonas</i>; moreover, strains BT552<sup>T</sup> and BT553<sup>T</sup> tightly clustered with <i>Sphingomonas melonis</i> DAPP-PG 224<sup>T</sup> (98.2 and 98.1 %) and <i>Sphingomonas aquatilis</i> JSS-7<sup>T</sup> (98.1 and 98.0 %), while strain KR1UV-12<sup>T</sup> clustered with <i>S. melonis</i> DAPP-PG 224<sup>T</sup> (97.9%) and <i>Sphingomonas rubra</i> BH3<sup>T</sup> (97.8%), respectively. The major cellular fatty acids of all three strains were summed feature 8 (C<sub>18:1</sub> ω7c/C<sub>18:1</sub> ω6c), comprising 44.7, 46.4 and 48.5%. Additionally, their respiratory quinone is Q-10, and polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids, sphingolipid and phosphatidylcholine. They all grow well at an optimum temperature of 25 °C, at pH 7. The draft genomes of strains BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup> measures 4 035 561 bp, 3 941 714 bp and 3 418 792 bp, respectively, comprising 3 804 3648 and 3236 coding sequences and 50, 48 and 45 RNA genes. The average nucleotide identity analysis and digital DNA-DNA hybridization values between BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup> and closely related <i>Sphingomonas</i> species range from 72.7 to 80.2% and 19.4 to 24.3%, respectively. Based on phenotypic, genotypic and chemotaxonomic data, these three strains BT552<sup>T</sup>, BT553<sup>T</sup> and KR1UV-12<sup>T</sup> represent three novel bacterial species within the genus <i>Sphingomonas</i> for which the names <i>Sphingomonas longa</i> sp. nov. (type strain BT552<sup>T</sup>= KCTC 82094<sup>T</sup> =NBRC 114993<sup>T</sup>), <i>Sphingomonas mollis</i> sp. nov. (type strain BT553<sup>T</sup> =KCTC 82095<sup>T</sup> =NBRC 114994<sup>T</sup>) and <i>Sphingomonas aurea</i> sp. nov. (type strain KR1UV-12<sup>T</sup> = KCTC 92959<sup>T</sup> = TBRC 18506<sup>T</sup>) are proposed.</p>\",\"PeriodicalId\":14390,\"journal\":{\"name\":\"International journal of systematic and evolutionary microbiology\",\"volume\":\"74 12\",\"pages\":\"\"},\"PeriodicalIF\":2.0000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"International journal of systematic and evolutionary microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1099/ijsem.0.006572\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"International journal of systematic and evolutionary microbiology","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1099/ijsem.0.006572","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"MICROBIOLOGY","Score":null,"Total":0}
Sphingomonas longa sp. nov., Sphingomonas mollis sp. nov. and Sphingomonas aurea sp. nov.: three novel Sphingomonas species isolated from soil.
Three Gram-negative, aerobic and non-motile bacterial strains, BT552T, BT553T and KR1UV-12T, were isolated from soil samples in Gwangju-si and Gangneung-si, the Republic of Korea. Phylogenetic analysis based on 16S rRNA gene sequence showed that strains BT552T, BT553T and KR1UV-12T clustered to a distinct clade within the family Sphingomonadaceae (order Sphingomonadales, class Alphaproteobacteria). The strains exhibited the highest genetic similarity with representatives of the genus Sphingomonas; moreover, strains BT552T and BT553T tightly clustered with Sphingomonas melonis DAPP-PG 224T (98.2 and 98.1 %) and Sphingomonas aquatilis JSS-7T (98.1 and 98.0 %), while strain KR1UV-12T clustered with S. melonis DAPP-PG 224T (97.9%) and Sphingomonas rubra BH3T (97.8%), respectively. The major cellular fatty acids of all three strains were summed feature 8 (C18:1 ω7c/C18:1 ω6c), comprising 44.7, 46.4 and 48.5%. Additionally, their respiratory quinone is Q-10, and polar lipids were diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine, phospholipids, sphingolipid and phosphatidylcholine. They all grow well at an optimum temperature of 25 °C, at pH 7. The draft genomes of strains BT552T, BT553T and KR1UV-12T measures 4 035 561 bp, 3 941 714 bp and 3 418 792 bp, respectively, comprising 3 804 3648 and 3236 coding sequences and 50, 48 and 45 RNA genes. The average nucleotide identity analysis and digital DNA-DNA hybridization values between BT552T, BT553T and KR1UV-12T and closely related Sphingomonas species range from 72.7 to 80.2% and 19.4 to 24.3%, respectively. Based on phenotypic, genotypic and chemotaxonomic data, these three strains BT552T, BT553T and KR1UV-12T represent three novel bacterial species within the genus Sphingomonas for which the names Sphingomonas longa sp. nov. (type strain BT552T= KCTC 82094T =NBRC 114993T), Sphingomonas mollis sp. nov. (type strain BT553T =KCTC 82095T =NBRC 114994T) and Sphingomonas aurea sp. nov. (type strain KR1UV-12T = KCTC 92959T = TBRC 18506T) are proposed.
期刊介绍：
Published by the Microbiology Society and owned by the International Committee on Systematics of Prokaryotes (ICSP), a committee of the Bacteriology and Applied Microbiology Division of the International Union of Microbiological Societies, International Journal of Systematic and Evolutionary Microbiology is the leading forum for the publication of novel microbial taxa and the ICSP’s official journal of record for prokaryotic names.
The journal welcomes high-quality research on all aspects of microbial evolution, phylogenetics and systematics, encouraging submissions on all prokaryotes, yeasts, microfungi, protozoa and microalgae across the full breadth of systematics including:
Identification, characterisation and culture preservation
Microbial evolution and biodiversity
Molecular environmental work with strong taxonomic or evolutionary content
Nomenclature
Taxonomy and phylogenetics.
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