miR-484作为乳腺癌中的“OncomiR”通过抑制凋亡基因促进肿瘤发生。

IF 3.4 2区 医学 Q2 ONCOLOGY
Annals of Surgical Oncology Pub Date : 2025-04-01 Epub Date: 2024-12-18 DOI:10.1245/s10434-024-16656-0
Reyhan Tahtasakal, Zuhal Hamurcu, Abdullah Bahadir Oz, Mustafa Balli, Halime Dana, Mustafa Gok, Venhar Cinar, Mevlude Inanc, Elif Funda Sener
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引用次数: 0

摘要

目的:乳腺癌(BC)是女性最常见的死亡原因之一。癌细胞逃避细胞凋亡,导致细胞不受控制地增殖。已知MicroRNAs (miRNAs)在癌细胞中调节凋亡。目的:本研究旨在确定miR-484在不同BC细胞中的变化及其与凋亡通路的关系。方法:选取42例患者的肿瘤及瘤旁健康组织标本,其中良性6例,恶性36例。根据肿瘤类型、肿瘤组织学分级、增殖指数和分子亚型对组织样本进行分类。采用实时荧光定量聚合酶链反应(qRT-PCR)检测基因表达水平,Western Blot检测蛋白水平。结果用δ - δ Ct法进行分析。结果:研究结果显示,miR-484在恶性肿瘤中的表达水平高于良性肿瘤,在肿瘤组织中的表达水平高于健康组织。此外,我们确定随着Ki-67水平、组织学分级和侵袭性的增加,miR-484表达水平也随之增加。与健康相邻组织相比,肿瘤组织中BCL2表达升高,Casp3和Casp9表达降低。因此,miR-484的表达与BCL2呈正相关,CASP3与CASP9的表达呈负相关。结论:我们的研究结果表明miR-484可能在BC中发挥onco-miR的作用。乳腺癌组织中miR-484和BCL2升高,Casp3降低,提示Casp9表达可能通过抑制BC细胞凋亡而增加不受控制的细胞增殖,并可能促进肿瘤进展。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
miR-484 as an "OncomiR" in Breast Cancer Promotes Tumorigenesis by Suppressing Apoptosis Genes.

Purpose: Breast cancer (BC) is one of the most common causes of death among females. Cancer cells escape from apoptosis, causing the cells to proliferate uncontrollably. MicroRNAs (miRNAs) are known to regulate apoptosis in cancer cells.

Objective: This study aimed to determine the change in miR-484 in different BC cells and its relationship with the apoptosis pathway.

Methods: In the study, tumor and healthy tissue samples adjacent to the tumor were collected from 42 patients (6 benign, 36 malignant). Tissue samples were classified according to tumor type, tumor histological grade, proliferation index, and molecular subtypes. Gene expression levels were determined by quantitative real-time polymerase chain reaction (qRT-PCR), and protein levels were determined using the Western Blot method. The results were analyzed using the delta-delta Ct method.

Results: Findings showed that miR-484 expression levels were higher in malignant tumors than in benign tumors, and higher in tumor tissues than healthy tissues. Additionally, it was determined that as Ki-67 levels and histological grade and aggressiveness increased, miR-484 expression levels also increased. In tumor tissue compared with healthy adjacent tissue, there was an increase in BCL2 expression and a decrease in Casp3 and Casp9 expression. Therefore, a positive correlation was found between miR-484 expression and BCL2, and a negative correlation was found between CASP3 and CASP9 expression.

Conclusion: Our results show that miR-484 may play a roll as an onco-miR in BC. Increased miR-484 and BCL2, and decreased Casp3, in breast tumor tissues suggest that Casp9 expression may increase uncontrolled cell proliferation by suppressing apoptosis in BC cells and may contribute to tumor progression.

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来源期刊
CiteScore
5.90
自引率
10.80%
发文量
1698
审稿时长
2.8 months
期刊介绍: The Annals of Surgical Oncology is the official journal of The Society of Surgical Oncology and is published for the Society by Springer. The Annals publishes original and educational manuscripts about oncology for surgeons from all specialities in academic and community settings.
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