新的4-苯乙烯香豆素衍生物作为生物分子的潜在荧光标记:在rna -鱼探针中的应用

IF 1 4区 生物学 Q4 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
R. Eustáquio, A. T. Caldeira, S. Arantes, A. Candeias, A. Pereira
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引用次数: 0

摘要

荧光显微镜是一种高灵敏度的成像技术,应用于各种科学领域,如细胞生物学、环境科学、医学和药学。它提供了使用多个荧光标记来可视化不同生物分子和生成彩色图像的优势,用于识别复杂生物分子结构中的特定成分和研究它们的相互作用。这些荧光标记产生化学稳定和最小破坏性的生物偶联物。氨基反应性荧光标记,由于其易于掺入生物分子,通常用于荧光原位杂交,组织化学,细胞示踪,受体结合和免疫化学等应用。然而,现有的流行荧光标签价格昂贵,使得香豆素衍生物成为开发明亮荧光团的潜在经济有效的解决方案。本研究合成了4-苯乙烯基香豆素衍生物的荧光标记,并对其作为生物分子潜在的有效荧光标记进行了评价。获得了12个新的荧光寡核苷酸探针,其中6个指向真核细胞rRNA区(EUK516), 6个指向原核细胞rRNA区(EUB338)。开发的荧光探针在HERCULES实验室(Évora大学,葡萄牙)生物降解和生物技术实验室培养的微生物上进行了测试,显示出作为RNA-FISH探针的有效性能。这些发现证明了新的4-苯乙烯香豆素衍生物在生物分子标记和生物成像方面的适用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

New 4-styrylcoumarin Derivatives as Potentials Fluorescent Labels for Biomolecules: Application in RNA-fish Probes

New 4-styrylcoumarin Derivatives as Potentials Fluorescent Labels for Biomolecules: Application in RNA-fish Probes

Fluorescence microscopy is a highly sensitive imaging technique used in various scientific fields such as cellular biology, environmental sciences, medicine, and pharmacy. It offers the advantage of using multiple fluorescent labels to visualize different biomolecules and generate multicolored images for identifying specific components within complex biomolecular structures and studying their interactions. These fluorescent labels create chemically stable and minimally disruptive bioconjugates. Amino-reactive fluorescent labels, due to their ease of incorporation into biomolecules, are commonly used in applications like fluorescence in situ hybridization, histochemistry, cell tracing, receptor binding, and immunochemistry. However, the existing popular fluorescent labels are expensive, making coumarin derivatives a potential cost-effective solution for developing bright fluorophores. In this study, the fluorescent 4-styrylcoumarin derivative labels were synthesized and evaluated as potentially effective fluorescent labels for biomolecules. Twelve new fluorescent oligonucleotide probes have been obtained, 6 directed to the rRNA region of eukaryotic cells (EUK516) and 6 directed to the rRNA region of prokaryotic cells (EUB338). The developed fluorescent probes were tested on microorganisms belonging to the culture collection of the Laboratory of Biodegradation and Biotechnology of the HERCULES Laboratory (University of Évora, Portugal), showing effective performance as RNA-FISH probes. These findings evidenced the applicability of the new 4-styrylcoumarin derivatives in labeling of biomolecules and bioimaging.

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来源期刊
Applied Biochemistry and Microbiology
Applied Biochemistry and Microbiology 生物-生物工程与应用微生物
CiteScore
1.70
自引率
12.50%
发文量
75
审稿时长
6-12 weeks
期刊介绍: Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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