Y. B. Zhu, W. Zhang, Y. L. Fan, Y. Y. Chen, J. L. Zhu, H. X. Lu
{"title":"赤铁矿（α-Fe2O3）纳米颗粒免疫层析检测牛奶中黄曲霉毒素M1和大肠杆菌O157:H7","authors":"Y. B. Zhu, W. Zhang, Y. L. Fan, Y. Y. Chen, J. L. Zhu, H. X. Lu","doi":"10.1134/S0003683824603111","DOIUrl":null,"url":null,"abstract":"<p>Two immunochromatographic assays based on hematite (α-Fe<sub>2</sub>O<sub>3</sub>) nanoparticles were developed for rapid detection of aflatoxin M1 (AFM1) and <i>Escherichia coli</i> O157:H7 in the milk. Hematite nanoparticles were prepared and utilized as colored signal labels. The competitive format was applied for AFM1 detection, while the sandwich format for <i>E. coli</i> O157:H7 determination. Under optimal conditions, the assay exhibited a visual detection limit of 0.10 ng/mL and quantitative determination limit of 0.017 ng/mL for AFM1. For <i>E. coli</i> O157:H7, a visual detection limit of 2.9 × 10<sup>5</sup> CFU/mL was achieved. The entire determination process could be accomplished within 15 min. Compared with traditional nanolabels, hematite nanoparticles showed advantages of facile preparation, high stability and easy bioconjugation. The developed assays provided effective and rapid screening strategies for AFM1 and <i>E. coli</i> O157:H7 in milk samples with high sensitivity and specificity.</p>","PeriodicalId":466,"journal":{"name":"Applied Biochemistry and Microbiology","volume":"60 6","pages":"1407 - 1415"},"PeriodicalIF":1.0000,"publicationDate":"2024-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Two Immunochromatographic Assays Based on Hematite (α-Fe2O3) Nanoparticles for Detection of Aflatoxin M1 and Escherichia coli O157:H7 in the Milk\",\"authors\":\"Y. B. Zhu, W. Zhang, Y. L. Fan, Y. Y. Chen, J. L. Zhu, H. X. Lu\",\"doi\":\"10.1134/S0003683824603111\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p>Two immunochromatographic assays based on hematite (α-Fe<sub>2</sub>O<sub>3</sub>) nanoparticles were developed for rapid detection of aflatoxin M1 (AFM1) and <i>Escherichia coli</i> O157:H7 in the milk. Hematite nanoparticles were prepared and utilized as colored signal labels. The competitive format was applied for AFM1 detection, while the sandwich format for <i>E. coli</i> O157:H7 determination. Under optimal conditions, the assay exhibited a visual detection limit of 0.10 ng/mL and quantitative determination limit of 0.017 ng/mL for AFM1. For <i>E. coli</i> O157:H7, a visual detection limit of 2.9 × 10<sup>5</sup> CFU/mL was achieved. The entire determination process could be accomplished within 15 min. Compared with traditional nanolabels, hematite nanoparticles showed advantages of facile preparation, high stability and easy bioconjugation. The developed assays provided effective and rapid screening strategies for AFM1 and <i>E. coli</i> O157:H7 in milk samples with high sensitivity and specificity.</p>\",\"PeriodicalId\":466,\"journal\":{\"name\":\"Applied Biochemistry and Microbiology\",\"volume\":\"60 6\",\"pages\":\"1407 - 1415\"},\"PeriodicalIF\":1.0000,\"publicationDate\":\"2024-10-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Applied Biochemistry and Microbiology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1134/S0003683824603111\",\"RegionNum\":4,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Applied Biochemistry and Microbiology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1134/S0003683824603111","RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Two Immunochromatographic Assays Based on Hematite (α-Fe2O3) Nanoparticles for Detection of Aflatoxin M1 and Escherichia coli O157:H7 in the Milk
Two immunochromatographic assays based on hematite (α-Fe2O3) nanoparticles were developed for rapid detection of aflatoxin M1 (AFM1) and Escherichia coli O157:H7 in the milk. Hematite nanoparticles were prepared and utilized as colored signal labels. The competitive format was applied for AFM1 detection, while the sandwich format for E. coli O157:H7 determination. Under optimal conditions, the assay exhibited a visual detection limit of 0.10 ng/mL and quantitative determination limit of 0.017 ng/mL for AFM1. For E. coli O157:H7, a visual detection limit of 2.9 × 105 CFU/mL was achieved. The entire determination process could be accomplished within 15 min. Compared with traditional nanolabels, hematite nanoparticles showed advantages of facile preparation, high stability and easy bioconjugation. The developed assays provided effective and rapid screening strategies for AFM1 and E. coli O157:H7 in milk samples with high sensitivity and specificity.
期刊介绍：
Applied Biochemistry and Microbiology is an international peer reviewed journal that publishes original articles on biochemistry and microbiology that have or may have practical applications. The studies include: enzymes and mechanisms of enzymatic reactions, biosynthesis of low and high molecular physiologically active compounds; the studies of their structure and properties; biogenesis and pathways of their regulation; metabolism of producers of biologically active compounds, biocatalysis in organic synthesis, applied genetics of microorganisms, applied enzymology; protein and metabolic engineering, biochemical bases of phytoimmunity, applied aspects of biochemical and immunochemical analysis; biodegradation of xenobiotics; biosensors; biomedical research (without clinical studies). Along with experimental works, the journal publishes descriptions of novel research techniques and reviews on selected topics.
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