Min Zhou, Xiaojie You, Jiake Zhang, Zi Ye, Jiahao Song, Bingdong Chen, Lieyang Fan, Jixuan Ma, Shijie Yang, Man Cheng, Weihong Chen
{"title":"miR-629-3p inhibits fine particulate matter exposure-induced lung function decline: results from the two-stage population study and in vitro study","authors":"Min Zhou, Xiaojie You, Jiake Zhang, Zi Ye, Jiahao Song, Bingdong Chen, Lieyang Fan, Jixuan Ma, Shijie Yang, Man Cheng, Weihong Chen","doi":"10.1016/j.envpol.2024.125535","DOIUrl":null,"url":null,"abstract":"MiRNAs were reported to play crucial roles in the pathogenesis of health damage caused by environmental pollutants. However, its potential role in fine particulate matter (PM<sub>2.5</sub>) exposure-induced lung function decline has rarely been elucidated. The present study was developed to profile specific miRNAs that were related to both PM<sub>2.5</sub> exposure and lung function decline, and to investigate the regulating role in PM<sub>2.5</sub> exposure-induced lung injury. Based on the Wuhan-Zhuhai cohort, in the discovery stage, plasma miRNA profiling for PM<sub>2.5</sub> exposure was conducted through next-generation sequencing among 60 participants with 120 observations in a repeated-measures design. Plasma miRNA profiling for lung function decline was conducted among 10 pairs of lung function decline incident cases and matched healthy controls. In the validating stage, miR-629-3p was selected from miRNAs that were related to both PM<sub>2.5</sub> exposure and lung function decline, and was measured by quantitative real-time PCR among 475 residents to validate its association with PM<sub>2.5</sub> exposure as well as lung function. <em>In vitro</em>, PM<sub>2.5</sub>-treated A549 and BEAS-2B cell models and miR-629-3p mimic/inhibitor models were used to explore the role and underlying mechanism of miR-629-3p on epithelial-mesenchymal transition (EMT) induced by PM<sub>2.5</sub> exposure. The two-stage population study found a negative association between personal PM<sub>2.5</sub> exposure and plasma miR-629-3p, while a positive association between miR-629-3p and lung function. <em>In vitro</em>, PM<sub>2.5</sub> treatment stimulated the expressions of EMT-related factors, accompanied by the activation of TGF-β1/TGF-βR1 signal pathway. Overexpression of miR-629-3p could inhibit PM<sub>2.5</sub>-induced TGF-βR1 expression and alleviate EMT process. And inhibition of miR-629-3p could promote TGF-βR1 expression and aggravate EMT process. In conclusion, miR-629-3p may alleviate the lung injury induced by PM<sub>2.5</sub> exposure through inhibiting TGF-β1/TGF-βR1 pathway.","PeriodicalId":311,"journal":{"name":"Environmental Pollution","volume":"48 1","pages":""},"PeriodicalIF":7.6000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Pollution","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.1016/j.envpol.2024.125535","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"ENVIRONMENTAL SCIENCES","Score":null,"Total":0}
miR-629-3p inhibits fine particulate matter exposure-induced lung function decline: results from the two-stage population study and in vitro study
MiRNAs were reported to play crucial roles in the pathogenesis of health damage caused by environmental pollutants. However, its potential role in fine particulate matter (PM2.5) exposure-induced lung function decline has rarely been elucidated. The present study was developed to profile specific miRNAs that were related to both PM2.5 exposure and lung function decline, and to investigate the regulating role in PM2.5 exposure-induced lung injury. Based on the Wuhan-Zhuhai cohort, in the discovery stage, plasma miRNA profiling for PM2.5 exposure was conducted through next-generation sequencing among 60 participants with 120 observations in a repeated-measures design. Plasma miRNA profiling for lung function decline was conducted among 10 pairs of lung function decline incident cases and matched healthy controls. In the validating stage, miR-629-3p was selected from miRNAs that were related to both PM2.5 exposure and lung function decline, and was measured by quantitative real-time PCR among 475 residents to validate its association with PM2.5 exposure as well as lung function. In vitro, PM2.5-treated A549 and BEAS-2B cell models and miR-629-3p mimic/inhibitor models were used to explore the role and underlying mechanism of miR-629-3p on epithelial-mesenchymal transition (EMT) induced by PM2.5 exposure. The two-stage population study found a negative association between personal PM2.5 exposure and plasma miR-629-3p, while a positive association between miR-629-3p and lung function. In vitro, PM2.5 treatment stimulated the expressions of EMT-related factors, accompanied by the activation of TGF-β1/TGF-βR1 signal pathway. Overexpression of miR-629-3p could inhibit PM2.5-induced TGF-βR1 expression and alleviate EMT process. And inhibition of miR-629-3p could promote TGF-βR1 expression and aggravate EMT process. In conclusion, miR-629-3p may alleviate the lung injury induced by PM2.5 exposure through inhibiting TGF-β1/TGF-βR1 pathway.
期刊介绍:
Environmental Pollution is an international peer-reviewed journal that publishes high-quality research papers and review articles covering all aspects of environmental pollution and its impacts on ecosystems and human health.
Subject areas include, but are not limited to:
• Sources and occurrences of pollutants that are clearly defined and measured in environmental compartments, food and food-related items, and human bodies;
• Interlinks between contaminant exposure and biological, ecological, and human health effects, including those of climate change;
• Contaminants of emerging concerns (including but not limited to antibiotic resistant microorganisms or genes, microplastics/nanoplastics, electronic wastes, light, and noise) and/or their biological, ecological, or human health effects;
• Laboratory and field studies on the remediation/mitigation of environmental pollution via new techniques and with clear links to biological, ecological, or human health effects;
• Modeling of pollution processes, patterns, or trends that is of clear environmental and/or human health interest;
• New techniques that measure and examine environmental occurrences, transport, behavior, and effects of pollutants within the environment or the laboratory, provided that they can be clearly used to address problems within regional or global environmental compartments.