Qi Xiao, Leyuan Zhang, Xueping Xu, Renyu Dai, Yingqing Tan, Xianbi Li, Dan Jin, Yanhua Fan
{"title":"氮代谢抑制剂 NmrA 可调控植物病原真菌大丽轮枝菌的分生孢子产生、黑色素合成和毒力。","authors":"Qi Xiao, Leyuan Zhang, Xueping Xu, Renyu Dai, Yingqing Tan, Xianbi Li, Dan Jin, Yanhua Fan","doi":"10.1094/PHYTO-07-24-0226-R","DOIUrl":null,"url":null,"abstract":"<p><p>NmrA homologs have been reported as conserved regulators of the nitrogen metabolite repression (NMR) in various fungi. Here, we identified a NmrA homolog in <i>Verticillium dahliae</i> and reported its functions in nitrogen utilization, growth and development, and pathogenesis. VdNmrA interacts with <i>V. dahliae</i> AreA protein and regulates the expression of a typical NCR target, the formamidase gene. <i>VdNmrA</i> deletion mutants exhibited significantly slower colony growth on media with Gln or Arg. Furthermore, <i>VdNmrA</i> deletion impaired hyphal growth, spore production, hyperosmotic stress tolerance, and melanin biosynthesis. Less ROS was produced in <i>VdNmrA</i> mutants, and the NADPH oxidase genes <i>noxA</i> and <i>noxB</i> showed lowered expression level compared to the wild type. <i>VdNmrA</i> mutants exhibited reduced virulence on cotton and <i>Arabidopsis</i> compared with wild type strains. Our results indicated that VdNmrA functioned as an NMR repressor and played important roles in nutrient utilization, fungal development, stress tolerance and pathogenicity in <i>V. dahliae</i>.</p>","PeriodicalId":20410,"journal":{"name":"Phytopathology","volume":" ","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"A nitrogen-metabolism inhibitor NmrA regulates conidial production, melanin synthesis and virulence in phytopathogenic fungus <i>Verticillium dahliae</i>.\",\"authors\":\"Qi Xiao, Leyuan Zhang, Xueping Xu, Renyu Dai, Yingqing Tan, Xianbi Li, Dan Jin, Yanhua Fan\",\"doi\":\"10.1094/PHYTO-07-24-0226-R\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>NmrA homologs have been reported as conserved regulators of the nitrogen metabolite repression (NMR) in various fungi. Here, we identified a NmrA homolog in <i>Verticillium dahliae</i> and reported its functions in nitrogen utilization, growth and development, and pathogenesis. VdNmrA interacts with <i>V. dahliae</i> AreA protein and regulates the expression of a typical NCR target, the formamidase gene. <i>VdNmrA</i> deletion mutants exhibited significantly slower colony growth on media with Gln or Arg. Furthermore, <i>VdNmrA</i> deletion impaired hyphal growth, spore production, hyperosmotic stress tolerance, and melanin biosynthesis. Less ROS was produced in <i>VdNmrA</i> mutants, and the NADPH oxidase genes <i>noxA</i> and <i>noxB</i> showed lowered expression level compared to the wild type. <i>VdNmrA</i> mutants exhibited reduced virulence on cotton and <i>Arabidopsis</i> compared with wild type strains. Our results indicated that VdNmrA functioned as an NMR repressor and played important roles in nutrient utilization, fungal development, stress tolerance and pathogenicity in <i>V. dahliae</i>.</p>\",\"PeriodicalId\":20410,\"journal\":{\"name\":\"Phytopathology\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-12-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Phytopathology\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://doi.org/10.1094/PHYTO-07-24-0226-R\",\"RegionNum\":2,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"PLANT SCIENCES\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Phytopathology","FirstCategoryId":"97","ListUrlMain":"https://doi.org/10.1094/PHYTO-07-24-0226-R","RegionNum":2,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"PLANT SCIENCES","Score":null,"Total":0}
A nitrogen-metabolism inhibitor NmrA regulates conidial production, melanin synthesis and virulence in phytopathogenic fungus Verticillium dahliae.
NmrA homologs have been reported as conserved regulators of the nitrogen metabolite repression (NMR) in various fungi. Here, we identified a NmrA homolog in Verticillium dahliae and reported its functions in nitrogen utilization, growth and development, and pathogenesis. VdNmrA interacts with V. dahliae AreA protein and regulates the expression of a typical NCR target, the formamidase gene. VdNmrA deletion mutants exhibited significantly slower colony growth on media with Gln or Arg. Furthermore, VdNmrA deletion impaired hyphal growth, spore production, hyperosmotic stress tolerance, and melanin biosynthesis. Less ROS was produced in VdNmrA mutants, and the NADPH oxidase genes noxA and noxB showed lowered expression level compared to the wild type. VdNmrA mutants exhibited reduced virulence on cotton and Arabidopsis compared with wild type strains. Our results indicated that VdNmrA functioned as an NMR repressor and played important roles in nutrient utilization, fungal development, stress tolerance and pathogenicity in V. dahliae.
期刊介绍:
Phytopathology publishes articles on fundamental research that advances understanding of the nature of plant diseases, the agents that cause them, their spread, the losses they cause, and measures that can be used to control them. Phytopathology considers manuscripts covering all aspects of plant diseases including bacteriology, host-parasite biochemistry and cell biology, biological control, disease control and pest management, description of new pathogen species description of new pathogen species, ecology and population biology, epidemiology, disease etiology, host genetics and resistance, mycology, nematology, plant stress and abiotic disorders, postharvest pathology and mycotoxins, and virology. Papers dealing mainly with taxonomy, such as descriptions of new plant pathogen taxa are acceptable if they include plant disease research results such as pathogenicity, host range, etc. Taxonomic papers that focus on classification, identification, and nomenclature below the subspecies level may also be submitted to Phytopathology.