Da Hyun Lee, Woo Hyun Sim, Chung Youl Park, Do Hyun Kim, Jun Hyeok Kim, Hyeon Min Kim, Sang Hoon Che, Wan Geun Park, Chae Sun Na, Sangrea Shim
{"title":"Ericaceae植物vacinium oldhamii Miquel的全线粒体基因组。","authors":"Da Hyun Lee, Woo Hyun Sim, Chung Youl Park, Do Hyun Kim, Jun Hyeok Kim, Hyeon Min Kim, Sang Hoon Che, Wan Geun Park, Chae Sun Na, Sangrea Shim","doi":"10.1080/23802359.2024.2438293","DOIUrl":null,"url":null,"abstract":"<p><p><i>Vaccinium oldhamii Miquel</i> 1866 is a deciduous shrub native to Northeast Asia, including Korea, China, and Japan<i>.</i> Its polyphenol-rich edible berries may aid in managing chronic diseases. Despite its importance, the mitogenome of this species remains understudied. This study assembled the complete mitogenome, consisting of 626,941 bp with 45.34% GC content, using NOVOPlasty and Illumina sequencing. The genome includes 38 protein-coding genes, 24 tRNA genes, and three rRNA genes. Phylogenetic analysis revealed a close relationship with <i>V. macrocarpon.</i> This mitogenome provides a valuable resource for phylogenomic studies and insights into evolutionary relationships within the <i>Vaccinium genus</i> and the Ericales order<i>. Vaccinium oldhamii</i> Miquel 1866 is a deciduous shrub in the Ericales and the Ericaceae and is native to Northeast Asia including Korea, China, and Japan. Particular emphasis has been placed on the edible berries of <i>Vaccinium</i> species and their use as dietary supplements. Specifically, the edible berries of <i>V. oldhamii</i> contain high levels of polyphenols, which may aid in preventing and managing chronic diseases. However, the mitogenome sequence of this species, a valuable resource for studying evolutionary relationships and genetic diversity within the <i>Vaccinium</i> genus, has not been thoroughly investigated. In this study, the complete mitochondrial genome sequence of <i>V. oldhamii</i> was assembled into 626,941 bp circular nucleotides with a GC content of 45.34% using NOVOPlasty and Illumina sequencing data. The comprehensive annotation for mitogenome of <i>V. oldhamii</i> revealed that the mitogenome comprises 38 known protein-coding genes, 24 tRNA genes, and three rRNA genes. Phylogenetic analysis using ML method showed that this species is closely related to the <i>V. macrocarpon</i>. This newly sequenced mitochondrial genome offers a valuable resource for phylogenomic analysis and will enhance our understanding of evolutionary relationships within the Ericales order and/or the <i>Vaccinium</i> genus.</p>","PeriodicalId":18647,"journal":{"name":"Mitochondrial DNA. 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This study assembled the complete mitogenome, consisting of 626,941 bp with 45.34% GC content, using NOVOPlasty and Illumina sequencing. The genome includes 38 protein-coding genes, 24 tRNA genes, and three rRNA genes. Phylogenetic analysis revealed a close relationship with <i>V. macrocarpon.</i> This mitogenome provides a valuable resource for phylogenomic studies and insights into evolutionary relationships within the <i>Vaccinium genus</i> and the Ericales order<i>. Vaccinium oldhamii</i> Miquel 1866 is a deciduous shrub in the Ericales and the Ericaceae and is native to Northeast Asia including Korea, China, and Japan. Particular emphasis has been placed on the edible berries of <i>Vaccinium</i> species and their use as dietary supplements. Specifically, the edible berries of <i>V. oldhamii</i> contain high levels of polyphenols, which may aid in preventing and managing chronic diseases. However, the mitogenome sequence of this species, a valuable resource for studying evolutionary relationships and genetic diversity within the <i>Vaccinium</i> genus, has not been thoroughly investigated. In this study, the complete mitochondrial genome sequence of <i>V. oldhamii</i> was assembled into 626,941 bp circular nucleotides with a GC content of 45.34% using NOVOPlasty and Illumina sequencing data. The comprehensive annotation for mitogenome of <i>V. oldhamii</i> revealed that the mitogenome comprises 38 known protein-coding genes, 24 tRNA genes, and three rRNA genes. Phylogenetic analysis using ML method showed that this species is closely related to the <i>V. macrocarpon</i>. This newly sequenced mitochondrial genome offers a valuable resource for phylogenomic analysis and will enhance our understanding of evolutionary relationships within the Ericales order and/or the <i>Vaccinium</i> genus.</p>\",\"PeriodicalId\":18647,\"journal\":{\"name\":\"Mitochondrial DNA. 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引用次数: 0
摘要
Vaccinium oldhamii Miquel 1866 是一种落叶灌木,原产于东北亚,包括韩国、中国和日本。其富含多酚的可食用浆果有助于控制慢性疾病。尽管该物种非常重要,但对其有丝分裂基因组的研究仍然不足。本研究利用 NOVOPlasty 和 Illumina 测序技术组装了完整的有丝分裂基因组,该基因组由 626,941 bp 组成,GC 含量为 45.34%。基因组包括 38 个蛋白质编码基因、24 个 tRNA 基因和 3 个 rRNA 基因。系统进化分析表明,它与 V. macrocarpon 关系密切。该有丝分裂基因组为系统发生组学研究提供了宝贵的资源,并有助于深入了解越橘属和越橘目中的进化关系。Vaccinium oldhamii Miquel 1866 是一种落叶灌木,属于酢浆草科和鸢尾科,原产于东北亚,包括朝鲜、中国和日本。人们特别重视越橘物种的可食用浆果及其作为膳食补充剂的用途。具体来说,老叶越橘的可食用浆果含有大量多酚,可帮助预防和控制慢性疾病。然而,该物种的有丝分裂基因组序列是研究越橘属内部进化关系和遗传多样性的宝贵资源,但尚未得到深入研究。在这项研究中,利用 NOVOPlasty 和 Illumina 测序数据,将 V. oldhamii 完整的线粒体基因组序列组装成 626,941 bp 的环状核苷酸,GC 含量为 45.34%。对 V. oldhamii 的有丝分裂基因组进行的全面注释显示,有丝分裂基因组包括 38 个已知的蛋白质编码基因、24 个 tRNA 基因和 3 个 rRNA 基因。利用 ML 方法进行的系统进化分析表明,该物种与 V. macrocarpon 亲缘关系密切。这一新测序的线粒体基因组为系统进化分析提供了宝贵的资源,并将加深我们对越橘目和/或越橘属内部进化关系的了解。
The complete mitochondrial genome of Vaccinium oldhamii Miquel, a plant of the Ericaceae family.
Vaccinium oldhamii Miquel 1866 is a deciduous shrub native to Northeast Asia, including Korea, China, and Japan. Its polyphenol-rich edible berries may aid in managing chronic diseases. Despite its importance, the mitogenome of this species remains understudied. This study assembled the complete mitogenome, consisting of 626,941 bp with 45.34% GC content, using NOVOPlasty and Illumina sequencing. The genome includes 38 protein-coding genes, 24 tRNA genes, and three rRNA genes. Phylogenetic analysis revealed a close relationship with V. macrocarpon. This mitogenome provides a valuable resource for phylogenomic studies and insights into evolutionary relationships within the Vaccinium genus and the Ericales order. Vaccinium oldhamii Miquel 1866 is a deciduous shrub in the Ericales and the Ericaceae and is native to Northeast Asia including Korea, China, and Japan. Particular emphasis has been placed on the edible berries of Vaccinium species and their use as dietary supplements. Specifically, the edible berries of V. oldhamii contain high levels of polyphenols, which may aid in preventing and managing chronic diseases. However, the mitogenome sequence of this species, a valuable resource for studying evolutionary relationships and genetic diversity within the Vaccinium genus, has not been thoroughly investigated. In this study, the complete mitochondrial genome sequence of V. oldhamii was assembled into 626,941 bp circular nucleotides with a GC content of 45.34% using NOVOPlasty and Illumina sequencing data. The comprehensive annotation for mitogenome of V. oldhamii revealed that the mitogenome comprises 38 known protein-coding genes, 24 tRNA genes, and three rRNA genes. Phylogenetic analysis using ML method showed that this species is closely related to the V. macrocarpon. This newly sequenced mitochondrial genome offers a valuable resource for phylogenomic analysis and will enhance our understanding of evolutionary relationships within the Ericales order and/or the Vaccinium genus.
期刊介绍:
This open access journal publishes high-quality and concise research articles reporting the sequence of full mitochondrial genomes, and short communications focusing on the physical, chemical, and biochemical aspects of mtDNA and proteins involved in mtDNA metabolism and interactions.