爱尔兰褐藻生物活性多糖岩藻聚糖和层藻素的生物活性研究。

IF 5.1 2区 生物学 Q2 CELL BIOLOGY
Cells Pub Date : 2024-11-22 DOI:10.3390/cells13231938
Shanmugapriya Karuppusamy, Janith Wanigasekara, Stephen Fitzpatrick, Henry Lyons, James Curtin, Gaurav Rajauria, Brijesh K Tiwari, Colm O'Donnell
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引用次数: 0

摘要

本研究旨在研究从爱尔兰褐藻(Laminaria digitata)和褐藻(Fucus vesiculosus)中提取的藻胶素(Laminaria digitata)和褐藻多糖(Fucus vesiculosus)的粗提和纯化样品的生物活性。采用2,2-二苯基-1-苦味酰肼和铁还原抗氧化能力测定来评价样品的抗氧化能力。采用环氧化酶抑制活性分析样品的抗炎潜力,并采用二肽基肽酶-4抑制剂筛选试验评估样品的抗糖尿病活性。使用Alamar Blue™测定不同类型癌细胞系的细胞毒性。与纯化后的样品和市售标准样品相比,海蜇素和岩藻糖聚糖的抗氧化活性显著提高(p < 0.05)。同样,与纯化样品相比,粗提物具有更强的抗炎和抗糖尿病作用。此外,粗层藻素和岩藻聚糖样品显示出较高的细胞毒活性。具体来说,流式细胞术分析证实,与2D单层细胞相比,使用不同癌细胞系的3D肿瘤球对生物活性化合物的抗性明显更高。根据观察到的生物活性值,所研究的层粘胶蛋白和岩藻多糖样品适合潜在的营养保健应用。未来的研究需要纯化所研究的生物活性化合物并提高其在食品和生物医学应用中的靶向治疗用途的选择性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Investigation of Biological Activity of Fucoidan and Laminarin as Bioactive Polysaccharides from Irish Brown Macroalgae.

This study aimed to investigate the biological activity of crude and purified laminarin and fucoidan samples extracted from Irish brown macroalgae species Laminaria digitata and Fucus vesiculosus. The antioxidant capacity of the samples was evaluated using the 2,2-diphenyl-1-picrylhydrazyl and ferric-reducing antioxidant power assays. The anti-inflammatory potential of the samples was analysed using the cyclooxygenases inhibition activity, and the antidiabetic activity was evaluated using a dipeptidyl peptidase-4 inhibitor screening assay. The cytotoxicity of the samples was measured using the Alamar Blue™ assay with different types of cancer cell lines. The crude laminarin and fucoidan samples exhibited higher antioxidant activity (p < 0.05) than the purified samples and commercial standards. Similarly, the crude extracts showed stronger anti-inflammatory and antidiabetic effects compared to the purified samples. Additionally, the crude laminarin and fucoidan samples showed higher cytotoxic activity. Specifically, as confirmed in the flow cytometry analysis, 3D tumour spheres using different cancer cell lines showed significantly higher resistance to bioactive compounds compared to 2D monolayer cells. The laminarin and fucoidan polysaccharide samples investigated are suitable for potential nutraceutical applications based on the biological activity values observed. Future research is necessary to purify the bioactive compounds investigated and improve their selectivity for targeted therapeutic uses in food and biomedical applications.

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来源期刊
Cells
Cells Biochemistry, Genetics and Molecular Biology-Biochemistry, Genetics and Molecular Biology (all)
CiteScore
9.90
自引率
5.00%
发文量
3472
审稿时长
16 days
期刊介绍: Cells (ISSN 2073-4409) is an international, peer-reviewed open access journal which provides an advanced forum for studies related to cell biology, molecular biology and biophysics. It publishes reviews, research articles, communications and technical notes. Our aim is to encourage scientists to publish their experimental and theoretical results in as much detail as possible. There is no restriction on the length of the papers. Full experimental and/or methodical details must be provided.
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