Jin-Min Pan, Jie Gao, Ming Jian Liu, Ke-Cheng Zhu, Hua-Yang Guo, Bao-Suo Liu, Nan Zhang, Dian-Chang Zhang
{"title":"从金鲳鱼Trachinotus ovatus中提取的Galectin-8的功能特征揭示了其广谱抗菌活性。","authors":"Jin-Min Pan, Jie Gao, Ming Jian Liu, Ke-Cheng Zhu, Hua-Yang Guo, Bao-Suo Liu, Nan Zhang, Dian-Chang Zhang","doi":"10.1007/s10126-024-10393-x","DOIUrl":null,"url":null,"abstract":"<div><p>Galectins exhibit a variety of biological functions through interactions with their ligands, including galactose and its derivatives. Tandem-repeat galectins, such as Galectin-8, can act as pattern recognition receptors to aggregate and neutralize bacterial pathogens. In this study, <i>Galectin-8</i> was identified in <i>Trachinotus ovatus</i> (golden pompano). Galectin-8 consists of two carbohydrate recognition domains (CRDs) connected by a linker region. Furthermore, molecular docking analysis suggests that the C-terminal CRD can bind galactose, mannose, and <i>N</i>-acetylglucosamine at similar binding sites. ToGal-8 expression levels were highest in the brain and blood of healthy <i>T. ovatus</i>. However, following infection with <i>Streptococcus agalactiae</i>, expression levels in the spleen and head kidney surged at 48 h, while liver expression significantly decreased by 96 h. Cytoplasmic Galectin-8 expression was upregulated after stimulation by peptidoglycan compared with lipopolysaccharide. Recombinant ToGal-8 (rToGal-8) was produced using a prokaryotic expression system. This protein could agglutinate red blood cells from rabbits, carp, and <i>T. ovatus</i> independently of Ca<sup>2+</sup>. Moreover, it was also effective in aggregating and eliminating several bacterial strains, such as <i>Staphylococcus aureus</i>, <i>Bacillus subtilis</i>, <i>Vibrio vulnificus</i>,<i> S. agalactiae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Aeromonas hydrophila</i>. Therefore, this study provides an in-depth analysis of the function of <i>T. ovatus</i> Galectin-8 for the first time, offering guidance for the healthy aquaculture of <i>T. ovatus</i>.</p></div>","PeriodicalId":690,"journal":{"name":"Marine Biotechnology","volume":"27 1","pages":""},"PeriodicalIF":2.6000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Functional Characterization of Galectin-8 from Golden Pompano Trachinotus ovatus Reveals Its Broad-Spectrum Antimicrobial Activity\",\"authors\":\"Jin-Min Pan, Jie Gao, Ming Jian Liu, Ke-Cheng Zhu, Hua-Yang Guo, Bao-Suo Liu, Nan Zhang, Dian-Chang Zhang\",\"doi\":\"10.1007/s10126-024-10393-x\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><p>Galectins exhibit a variety of biological functions through interactions with their ligands, including galactose and its derivatives. Tandem-repeat galectins, such as Galectin-8, can act as pattern recognition receptors to aggregate and neutralize bacterial pathogens. In this study, <i>Galectin-8</i> was identified in <i>Trachinotus ovatus</i> (golden pompano). Galectin-8 consists of two carbohydrate recognition domains (CRDs) connected by a linker region. Furthermore, molecular docking analysis suggests that the C-terminal CRD can bind galactose, mannose, and <i>N</i>-acetylglucosamine at similar binding sites. ToGal-8 expression levels were highest in the brain and blood of healthy <i>T. ovatus</i>. However, following infection with <i>Streptococcus agalactiae</i>, expression levels in the spleen and head kidney surged at 48 h, while liver expression significantly decreased by 96 h. Cytoplasmic Galectin-8 expression was upregulated after stimulation by peptidoglycan compared with lipopolysaccharide. Recombinant ToGal-8 (rToGal-8) was produced using a prokaryotic expression system. This protein could agglutinate red blood cells from rabbits, carp, and <i>T. ovatus</i> independently of Ca<sup>2+</sup>. Moreover, it was also effective in aggregating and eliminating several bacterial strains, such as <i>Staphylococcus aureus</i>, <i>Bacillus subtilis</i>, <i>Vibrio vulnificus</i>,<i> S. agalactiae</i>, <i>Pseudomonas aeruginosa</i>, and <i>Aeromonas hydrophila</i>. Therefore, this study provides an in-depth analysis of the function of <i>T. ovatus</i> Galectin-8 for the first time, offering guidance for the healthy aquaculture of <i>T. ovatus</i>.</p></div>\",\"PeriodicalId\":690,\"journal\":{\"name\":\"Marine Biotechnology\",\"volume\":\"27 1\",\"pages\":\"\"},\"PeriodicalIF\":2.6000,\"publicationDate\":\"2024-12-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Marine Biotechnology\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://link.springer.com/article/10.1007/s10126-024-10393-x\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q3\",\"JCRName\":\"BIOTECHNOLOGY & APPLIED MICROBIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Marine Biotechnology","FirstCategoryId":"99","ListUrlMain":"https://link.springer.com/article/10.1007/s10126-024-10393-x","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q3","JCRName":"BIOTECHNOLOGY & APPLIED MICROBIOLOGY","Score":null,"Total":0}
Functional Characterization of Galectin-8 from Golden Pompano Trachinotus ovatus Reveals Its Broad-Spectrum Antimicrobial Activity
Galectins exhibit a variety of biological functions through interactions with their ligands, including galactose and its derivatives. Tandem-repeat galectins, such as Galectin-8, can act as pattern recognition receptors to aggregate and neutralize bacterial pathogens. In this study, Galectin-8 was identified in Trachinotus ovatus (golden pompano). Galectin-8 consists of two carbohydrate recognition domains (CRDs) connected by a linker region. Furthermore, molecular docking analysis suggests that the C-terminal CRD can bind galactose, mannose, and N-acetylglucosamine at similar binding sites. ToGal-8 expression levels were highest in the brain and blood of healthy T. ovatus. However, following infection with Streptococcus agalactiae, expression levels in the spleen and head kidney surged at 48 h, while liver expression significantly decreased by 96 h. Cytoplasmic Galectin-8 expression was upregulated after stimulation by peptidoglycan compared with lipopolysaccharide. Recombinant ToGal-8 (rToGal-8) was produced using a prokaryotic expression system. This protein could agglutinate red blood cells from rabbits, carp, and T. ovatus independently of Ca2+. Moreover, it was also effective in aggregating and eliminating several bacterial strains, such as Staphylococcus aureus, Bacillus subtilis, Vibrio vulnificus, S. agalactiae, Pseudomonas aeruginosa, and Aeromonas hydrophila. Therefore, this study provides an in-depth analysis of the function of T. ovatus Galectin-8 for the first time, offering guidance for the healthy aquaculture of T. ovatus.
期刊介绍:
Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.