从金鲳鱼Trachinotus ovatus中提取的Galectin-8的功能特征揭示了其广谱抗菌活性。

IF 2.6 3区 生物学 Q3 BIOTECHNOLOGY & APPLIED MICROBIOLOGY
Jin-Min Pan, Jie Gao, Ming Jian Liu, Ke-Cheng Zhu, Hua-Yang Guo, Bao-Suo Liu, Nan Zhang, Dian-Chang Zhang
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引用次数: 0

摘要

半乳糖凝集素通过与其配体(包括半乳糖及其衍生物)的相互作用表现出多种生物学功能。串联重复半乳糖凝集素,如半乳糖凝集素-8,可以作为模式识别受体聚集和中和细菌病原体。本研究从金鲳鱼(Trachinotus ovatus)中鉴定出半乳糖凝集素-8。半乳糖凝集素-8由两个碳水化合物识别结构域(CRDs)组成,由一个连接体区域连接。此外,分子对接分析表明,c端CRD可以在相似的结合位点结合半乳糖、甘露糖和n -乙酰氨基葡萄糖。健康卵圆体的脑组织和血液中ToGal-8表达水平最高。然而,在感染无乳链球菌后,脾脏和头肾的表达水平在48 h时激增,而肝脏的表达水平在96 h时显著下降。与脂多糖相比,肽聚糖刺激后细胞质半乳糖凝集素-8的表达上调。重组ToGal-8 (rToGal-8)通过原核表达系统得到。该蛋白能独立于Ca2+而凝集兔、鲤鱼和卵鲫红细胞。对金黄色葡萄球菌、枯草芽孢杆菌、创伤弧菌、无乳葡萄球菌、铜绿假单胞菌、嗜水气单胞菌等多种细菌均有聚集和杀灭作用。因此,本研究首次对卵形鱼半凝集素-8的功能进行了深入分析,为卵形鱼的健康养殖提供指导。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Functional Characterization of Galectin-8 from Golden Pompano Trachinotus ovatus Reveals Its Broad-Spectrum Antimicrobial Activity

Functional Characterization of Galectin-8 from Golden Pompano Trachinotus ovatus Reveals Its Broad-Spectrum Antimicrobial Activity

Galectins exhibit a variety of biological functions through interactions with their ligands, including galactose and its derivatives. Tandem-repeat galectins, such as Galectin-8, can act as pattern recognition receptors to aggregate and neutralize bacterial pathogens. In this study, Galectin-8 was identified in Trachinotus ovatus (golden pompano). Galectin-8 consists of two carbohydrate recognition domains (CRDs) connected by a linker region. Furthermore, molecular docking analysis suggests that the C-terminal CRD can bind galactose, mannose, and N-acetylglucosamine at similar binding sites. ToGal-8 expression levels were highest in the brain and blood of healthy T. ovatus. However, following infection with Streptococcus agalactiae, expression levels in the spleen and head kidney surged at 48 h, while liver expression significantly decreased by 96 h. Cytoplasmic Galectin-8 expression was upregulated after stimulation by peptidoglycan compared with lipopolysaccharide. Recombinant ToGal-8 (rToGal-8) was produced using a prokaryotic expression system. This protein could agglutinate red blood cells from rabbits, carp, and T. ovatus independently of Ca2+. Moreover, it was also effective in aggregating and eliminating several bacterial strains, such as Staphylococcus aureus, Bacillus subtilis, Vibrio vulnificus, S. agalactiae, Pseudomonas aeruginosa, and Aeromonas hydrophila. Therefore, this study provides an in-depth analysis of the function of T. ovatus Galectin-8 for the first time, offering guidance for the healthy aquaculture of T. ovatus.

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来源期刊
Marine Biotechnology
Marine Biotechnology 工程技术-海洋与淡水生物学
CiteScore
4.80
自引率
3.30%
发文量
95
审稿时长
2 months
期刊介绍: Marine Biotechnology welcomes high-quality research papers presenting novel data on the biotechnology of aquatic organisms. The journal publishes high quality papers in the areas of molecular biology, genomics, proteomics, cell biology, and biochemistry, and particularly encourages submissions of papers related to genome biology such as linkage mapping, large-scale gene discoveries, QTL analysis, physical mapping, and comparative and functional genome analysis. Papers on technological development and marine natural products should demonstrate innovation and novel applications.
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