Franklin Perez, Nesha Andoy, Uyan Tran Thao Hua, Keiko Yoshioka, Ruby May Arana Sullan
{"title":"枯草芽孢杆菌的适应性反应是纳米塑料毒性差异的基础,对根部定殖具有影响","authors":"Franklin Perez, Nesha Andoy, Uyan Tran Thao Hua, Keiko Yoshioka, Ruby May Arana Sullan","doi":"10.1039/d4en00936c","DOIUrl":null,"url":null,"abstract":"Positively charged nanoplastics are more toxic to microorganisms than their negatively charged counterparts, prompting further investigation into their antimicrobial properties. While many studies have shown that positively charged nanoplastics bind to bacteria, the fate of these nanoplastic coatings during bacterial growth remains unclear. Here, we report how amine-modified polystyrene nanoplastics (PS-NH<small><sub>2</sub></small>) reduce the viability of the plant growth-promoting rhizobacterium <em>Bacillus subtilis</em> and impair its ability to colonize plant roots. We found that upon exposure to PS-NH<small><sub>2</sub></small>, the nanoplastics form stable, multilayer coatings on the surface of the bacteria. In response,<em> B. subtilis</em> initiates processes to remove these nanoplastics—a behavior heavily influenced by their growth environment, whether at air or liquid interfaces. Consequently, we observed differential toxicity under varying growth conditions. Using tomato plant as a model system, we found that these nanoplastics severely inhibit bacterial attachment to plant roots. Our results demonstrate that nanoplastics can disrupt beneficial interactions between soil bacteria and plants, potentially compromising the effectiveness of microbial biofertilizers. Given that current practices introduce large amounts of plastics into agricultural areas, the adverse effects of nanoplastic pollution need to be mitigated","PeriodicalId":73,"journal":{"name":"Environmental Science: Nano","volume":"3 1","pages":""},"PeriodicalIF":5.8000,"publicationDate":"2024-12-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Adaptive responses of Bacillus subtilis underlie differential nanoplastic toxicity with implications for root colonization\",\"authors\":\"Franklin Perez, Nesha Andoy, Uyan Tran Thao Hua, Keiko Yoshioka, Ruby May Arana Sullan\",\"doi\":\"10.1039/d4en00936c\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"Positively charged nanoplastics are more toxic to microorganisms than their negatively charged counterparts, prompting further investigation into their antimicrobial properties. While many studies have shown that positively charged nanoplastics bind to bacteria, the fate of these nanoplastic coatings during bacterial growth remains unclear. Here, we report how amine-modified polystyrene nanoplastics (PS-NH<small><sub>2</sub></small>) reduce the viability of the plant growth-promoting rhizobacterium <em>Bacillus subtilis</em> and impair its ability to colonize plant roots. We found that upon exposure to PS-NH<small><sub>2</sub></small>, the nanoplastics form stable, multilayer coatings on the surface of the bacteria. In response,<em> B. subtilis</em> initiates processes to remove these nanoplastics—a behavior heavily influenced by their growth environment, whether at air or liquid interfaces. Consequently, we observed differential toxicity under varying growth conditions. Using tomato plant as a model system, we found that these nanoplastics severely inhibit bacterial attachment to plant roots. Our results demonstrate that nanoplastics can disrupt beneficial interactions between soil bacteria and plants, potentially compromising the effectiveness of microbial biofertilizers. Given that current practices introduce large amounts of plastics into agricultural areas, the adverse effects of nanoplastic pollution need to be mitigated\",\"PeriodicalId\":73,\"journal\":{\"name\":\"Environmental Science: Nano\",\"volume\":\"3 1\",\"pages\":\"\"},\"PeriodicalIF\":5.8000,\"publicationDate\":\"2024-12-17\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Environmental Science: Nano\",\"FirstCategoryId\":\"6\",\"ListUrlMain\":\"https://doi.org/10.1039/d4en00936c\",\"RegionNum\":2,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"CHEMISTRY, MULTIDISCIPLINARY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Environmental Science: Nano","FirstCategoryId":"6","ListUrlMain":"https://doi.org/10.1039/d4en00936c","RegionNum":2,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"CHEMISTRY, MULTIDISCIPLINARY","Score":null,"Total":0}
Adaptive responses of Bacillus subtilis underlie differential nanoplastic toxicity with implications for root colonization
Positively charged nanoplastics are more toxic to microorganisms than their negatively charged counterparts, prompting further investigation into their antimicrobial properties. While many studies have shown that positively charged nanoplastics bind to bacteria, the fate of these nanoplastic coatings during bacterial growth remains unclear. Here, we report how amine-modified polystyrene nanoplastics (PS-NH2) reduce the viability of the plant growth-promoting rhizobacterium Bacillus subtilis and impair its ability to colonize plant roots. We found that upon exposure to PS-NH2, the nanoplastics form stable, multilayer coatings on the surface of the bacteria. In response, B. subtilis initiates processes to remove these nanoplastics—a behavior heavily influenced by their growth environment, whether at air or liquid interfaces. Consequently, we observed differential toxicity under varying growth conditions. Using tomato plant as a model system, we found that these nanoplastics severely inhibit bacterial attachment to plant roots. Our results demonstrate that nanoplastics can disrupt beneficial interactions between soil bacteria and plants, potentially compromising the effectiveness of microbial biofertilizers. Given that current practices introduce large amounts of plastics into agricultural areas, the adverse effects of nanoplastic pollution need to be mitigated
期刊介绍:
Environmental Science: Nano serves as a comprehensive and high-impact peer-reviewed source of information on the design and demonstration of engineered nanomaterials for environment-based applications. It also covers the interactions between engineered, natural, and incidental nanomaterials with biological and environmental systems. This scope includes, but is not limited to, the following topic areas:
Novel nanomaterial-based applications for water, air, soil, food, and energy sustainability
Nanomaterial interactions with biological systems and nanotoxicology
Environmental fate, reactivity, and transformations of nanoscale materials
Nanoscale processes in the environment
Sustainable nanotechnology including rational nanomaterial design, life cycle assessment, risk/benefit analysis