IF 7.3 1区 工程技术 Q1 INSTRUMENTS & INSTRUMENTATION
Minruihong Wang, Jie Zhang, Xiao Chen, Yimin Li, Xukun Huang, Junbo Wang, Yueying Li, Xiaoye Huo, Jian Chen
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引用次数: 0

摘要

微流体阻抗流式细胞仪已广泛应用于白细胞的鉴别和计数,但由于阻抗检测通量和灵敏度之间的权衡问题,该技术面临瓶颈。本研究报道了一种基于虚拟收缩微通道的微流体阻抗流式细胞仪,虚拟收缩微通道是通过导电样品流和绝缘鞘液的交叉流动构建的,鞘液下方有用于阻抗测量的微电极。与传统的机械收缩微通道相比,这种虚拟微通道能有效避免细胞与微通道壁的直接物理接触,从而保持高通量,并能显著缩小阻抗检测区域的体积,提高灵敏度。利用所开发的微流体阻抗流式细胞仪,通过使用递归神经网络,检测了 K562、Jurkat 和 HL-60 三种白血病细胞株的阻抗脉冲,分化准确率达到 99.8%。此外,还检测了来自三名捐献者的四种白细胞亚群(中性粒细胞、嗜酸性粒细胞、单核细胞和淋巴细胞)的阻抗脉冲,分类准确率≥99.2%。基于纯化白细胞建立了分类网络模型,并将其应用于两种白细胞混合物的阻抗脉冲,结果显示四种白细胞亚群的比例分布在理论范围内。这些结果表明,所开发的基于虚拟收缩微通道的微流体阻抗流式细胞仪可实现高检测通量和高灵敏度,在临床诊断和血液分析方面具有巨大潜力。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Microfluidic impedance flow cytometer leveraging virtual constriction microchannel and its application in leukocyte differential.

Microfluidic impedance flow cytometry has been widely used in leukocyte differential and counting, but it faces a bottleneck due to the trade-off between impedance detection throughput and sensitivity. In this study, a microfluidic impedance flow cytometer based on a virtual constriction microchannel was reported, in which the virtual constriction microchannel was constructed by crossflow of conductive sample and insulated sheath fluids with underneath micro-electrodes for impedance measurements. Compared to conventional mechanical constriction microchannels, this virtual counterpart could effectively avoid direct physical contact between cells and the microchannel walls to maintain high throughputs, and significantly reduce the volume of the impedance detection region for sensitivity improvements. Using the developed microfluidic impedance flow cytometer, impedance pulses of three leukemia cell lines, K562, Jurkat, and HL-60, were detected, achieving a 99.8% differentiation accuracy through the use of a recurrent neural network. Furthermore, impedance pulses of four white blood cell subpopulations (neutrophils, eosinophils, monocytes, and lymphocytes) from three donors were detected, achieving a classification accuracy of ≥99.2%. A classification network model was established based on purified white blood cell and applied to impedance pulses of two white blood cell mixtures, resulting in proportional distributions of four leukocyte subpopulations within theoretical ranges. These results indicated that the developed microfluidic impedance flow cytometer based on the virtual constriction microchannel could achieve both high detection throughput and high sensitivity, showing great potentials for clinical diagnostics and blood analysis.

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来源期刊
Microsystems & Nanoengineering
Microsystems & Nanoengineering Materials Science-Materials Science (miscellaneous)
CiteScore
12.00
自引率
3.80%
发文量
123
审稿时长
20 weeks
期刊介绍: Microsystems & Nanoengineering is a comprehensive online journal that focuses on the field of Micro and Nano Electro Mechanical Systems (MEMS and NEMS). It provides a platform for researchers to share their original research findings and review articles in this area. The journal covers a wide range of topics, from fundamental research to practical applications. Published by Springer Nature, in collaboration with the Aerospace Information Research Institute, Chinese Academy of Sciences, and with the support of the State Key Laboratory of Transducer Technology, it is an esteemed publication in the field. As an open access journal, it offers free access to its content, allowing readers from around the world to benefit from the latest developments in MEMS and NEMS.
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