Carlos A Montoya, Dulantha Ulluwishewa, Natalie G Ahlborn, Nicole C Roy, Warren C McNabb
{"title":"牛乳的热处理会影响乳糖的胃排空,但不会影响生长猪作为成人模型中乳糖在小肠中的明显消失。","authors":"Carlos A Montoya, Dulantha Ulluwishewa, Natalie G Ahlborn, Nicole C Roy, Warren C McNabb","doi":"10.1016/j.tjnut.2024.12.008","DOIUrl":null,"url":null,"abstract":"<p><strong>Background: </strong>Heat treatment influences gastric emptying of proteins and lipids in bovine milk. Whether heat treatment influences lactose gastric emptying and small intestinal lactose disappearance remains unknown.</p><p><strong>Objective: </strong>This study aimed to determine the gastric emptying of lactose and its disappearance from the small intestine of the growing pig as a model for the adult human.</p><p><strong>Methods: </strong>After 10 days of adaptation, fasted nine-week-old pigs (n=48) consumed 500 mL of pasteurized or ultra-high-temperature (UHT) bovine milk before being euthanized at 20, 60, 120, or 180 min post-feeding. Gastric, small, and large intestinal contents were collected to determine the gastric emptying of lactose, its apparent small intestinal disappearance, and its release into the large intestine. Portal vein blood samples were also collected to measure galactose concentration over time. Data were analyzed using non-linear and polynomial models.</p><p><strong>Results: </strong>The gastric emptying of lactose was faster for pigs fed pasteurized milk than those fed UHT milk (P ≤ 0.05). For example, 14±2.9 and 24±2.2% (mean ± standard error) of lactose, respectively, were retained in the stomach at 60 min post-feeding. The apparent small intestinal disappearance of lactose increased to 88±2.4% over time, but it did not differ between milk types. Similarly, the plasma galactose concentration in the portal vein increased during the first post-feeding hour but did not differ between milk types. Lactose reached the large intestine during the first 20 min post-feeding, and the amount of lactose released into the large intestine did not change over time (523 μmol on average across post-feeding times after back transformation of 6.26±0.37) or between milk types.</p><p><strong>Conclusions: </strong>Heat treatment influenced the gastric emptying of lactose but did not impact subsequent small intestinal apparent disappearance and portal blood plasma galactose appearance.</p>","PeriodicalId":16620,"journal":{"name":"Journal of Nutrition","volume":" ","pages":""},"PeriodicalIF":3.7000,"publicationDate":"2024-12-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Heat treatment of bovine milk influences gastric emptying of lactose but not its apparent small intestinal disappearance in the growing pig as a model for the adult human.\",\"authors\":\"Carlos A Montoya, Dulantha Ulluwishewa, Natalie G Ahlborn, Nicole C Roy, Warren C McNabb\",\"doi\":\"10.1016/j.tjnut.2024.12.008\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><strong>Background: </strong>Heat treatment influences gastric emptying of proteins and lipids in bovine milk. Whether heat treatment influences lactose gastric emptying and small intestinal lactose disappearance remains unknown.</p><p><strong>Objective: </strong>This study aimed to determine the gastric emptying of lactose and its disappearance from the small intestine of the growing pig as a model for the adult human.</p><p><strong>Methods: </strong>After 10 days of adaptation, fasted nine-week-old pigs (n=48) consumed 500 mL of pasteurized or ultra-high-temperature (UHT) bovine milk before being euthanized at 20, 60, 120, or 180 min post-feeding. Gastric, small, and large intestinal contents were collected to determine the gastric emptying of lactose, its apparent small intestinal disappearance, and its release into the large intestine. Portal vein blood samples were also collected to measure galactose concentration over time. Data were analyzed using non-linear and polynomial models.</p><p><strong>Results: </strong>The gastric emptying of lactose was faster for pigs fed pasteurized milk than those fed UHT milk (P ≤ 0.05). For example, 14±2.9 and 24±2.2% (mean ± standard error) of lactose, respectively, were retained in the stomach at 60 min post-feeding. The apparent small intestinal disappearance of lactose increased to 88±2.4% over time, but it did not differ between milk types. Similarly, the plasma galactose concentration in the portal vein increased during the first post-feeding hour but did not differ between milk types. Lactose reached the large intestine during the first 20 min post-feeding, and the amount of lactose released into the large intestine did not change over time (523 μmol on average across post-feeding times after back transformation of 6.26±0.37) or between milk types.</p><p><strong>Conclusions: </strong>Heat treatment influenced the gastric emptying of lactose but did not impact subsequent small intestinal apparent disappearance and portal blood plasma galactose appearance.</p>\",\"PeriodicalId\":16620,\"journal\":{\"name\":\"Journal of Nutrition\",\"volume\":\" \",\"pages\":\"\"},\"PeriodicalIF\":3.7000,\"publicationDate\":\"2024-12-13\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Journal of Nutrition\",\"FirstCategoryId\":\"3\",\"ListUrlMain\":\"https://doi.org/10.1016/j.tjnut.2024.12.008\",\"RegionNum\":3,\"RegionCategory\":\"医学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"NUTRITION & DIETETICS\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Journal of Nutrition","FirstCategoryId":"3","ListUrlMain":"https://doi.org/10.1016/j.tjnut.2024.12.008","RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"NUTRITION & DIETETICS","Score":null,"Total":0}
Heat treatment of bovine milk influences gastric emptying of lactose but not its apparent small intestinal disappearance in the growing pig as a model for the adult human.
Background: Heat treatment influences gastric emptying of proteins and lipids in bovine milk. Whether heat treatment influences lactose gastric emptying and small intestinal lactose disappearance remains unknown.
Objective: This study aimed to determine the gastric emptying of lactose and its disappearance from the small intestine of the growing pig as a model for the adult human.
Methods: After 10 days of adaptation, fasted nine-week-old pigs (n=48) consumed 500 mL of pasteurized or ultra-high-temperature (UHT) bovine milk before being euthanized at 20, 60, 120, or 180 min post-feeding. Gastric, small, and large intestinal contents were collected to determine the gastric emptying of lactose, its apparent small intestinal disappearance, and its release into the large intestine. Portal vein blood samples were also collected to measure galactose concentration over time. Data were analyzed using non-linear and polynomial models.
Results: The gastric emptying of lactose was faster for pigs fed pasteurized milk than those fed UHT milk (P ≤ 0.05). For example, 14±2.9 and 24±2.2% (mean ± standard error) of lactose, respectively, were retained in the stomach at 60 min post-feeding. The apparent small intestinal disappearance of lactose increased to 88±2.4% over time, but it did not differ between milk types. Similarly, the plasma galactose concentration in the portal vein increased during the first post-feeding hour but did not differ between milk types. Lactose reached the large intestine during the first 20 min post-feeding, and the amount of lactose released into the large intestine did not change over time (523 μmol on average across post-feeding times after back transformation of 6.26±0.37) or between milk types.
Conclusions: Heat treatment influenced the gastric emptying of lactose but did not impact subsequent small intestinal apparent disappearance and portal blood plasma galactose appearance.
期刊介绍:
The Journal of Nutrition (JN/J Nutr) publishes peer-reviewed original research papers covering all aspects of experimental nutrition in humans and other animal species; special articles such as reviews and biographies of prominent nutrition scientists; and issues, opinions, and commentaries on controversial issues in nutrition. Supplements are frequently published to provide extended discussion of topics of special interest.