IF 3.6 3区 生物学 Q1 ZOOLOGY
Emily D Vu, Sijun Liu, Bryony C Bonning
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引用次数: 0

摘要

小蜂巢甲虫(SHB),Aethina tumida Murray,是蜜蜂的一种入侵害虫。这种甲虫不仅以蜂巢内的蜜蜂资源(如蜂蜜和花粉)为食,而且还以蜜蜂的雏蜂和死蜂为食。人们对这种甲虫与蜜蜂的密切寄生关系对病毒传播的影响知之甚少。我们的目的是描述 SHB 病毒组的特征,以确定有可能用于这种害虫生物防治的 SHB 病毒。我们通过对从多个地理区域收集的 SHB 的转录组和小 RNA 进行测序,确定了 SHB 病毒组的特征:我们从美国中西部(伊利诺伊州、俄亥俄州)和南部(佛罗里达州、德克萨斯州)以及南非收集了 SHB 的成虫和幼虫。对来自佛罗里达州和俄亥俄州的甲虫成虫、佛罗里达州的幼虫以及 SHB 衍生细胞系(BCIRL-AtumEN-1129)的小 RNA 进行了测序。通过 BLASTx 对组装的转录本进行了注释。在野外捕获的甲虫成虫以及来自南非的成虫和幼虫中,确定了一种推测的新型噬菌体病毒(目:Elliovirales,前身为Bunyavirales)所有三个基因组片段的近乎完整的序列。此外,在所有样本(包括 SHB 衍生细胞系)中都检测到了整合到 SHB 基因组中的不同噬病毒的部分糖蛋白序列的转录本。还检测到了来自整合糖蛋白序列的明显与 PIWI 相互作用的 RNA。推定的现存噬菌体病毒是否会在SHB中复制以及是否有可能成为SHB的生物防治剂仍有待确定。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Phasmavirus-derived genome sequences and endogenous viral element identified in the small hive beetle, Aethina tumida Murray.

The small hive beetle (SHB), Aethina tumida Murray is an invasive pest of the honey bee. This beetle feeds not only on bee resources within the hive such as honey and pollen, but also on bee brood and dead bees. The impact of this beetle's intimate parasitic association with the honey bee on virus transmission is poorly understood. We aimed to characterize the virome of SHB to identify SHB viruses with potential for use in biological control of this pest. We characterized the virome of SHB by sequencing the transcriptomes and small RNAs of SHB collected from multiple geographical regions: Adult and larval SHB were collected from midwestern- (Illinois, Ohio) and southern- (Florida, Texas) states of the USA, and from South Africa. Small RNAs were sequenced for adult beetles from Florida and Ohio, for larvae from Florida, and for an SHB-derived cell line (BCIRL-AtumEN-1129). Assembled transcripts were annotated by BLASTx. In field-caught adult beetles and adults and larvae from South Africa, the near-complete sequences for all three genomic segments of a putative novel phasmavirus (order: Elliovirales, formerly Bunyavirales) were identified. In addition, transcripts from a partial glycoprotein sequence from a different phasmavirus integrated into the genome of SHB were detected in all samples, including the SHB-derived cell line. Apparent PIWI-interacting RNAs derived from the integrated glycoprotein sequence were also detected. Whether the putative extant phasmavirus replicates in SHB and has potential as a biological control agent for SHB remain to be determined.

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来源期刊
CiteScore
6.10
自引率
5.90%
发文量
94
审稿时长
1 months
期刊介绍: The Journal of Invertebrate Pathology presents original research articles and notes on the induction and pathogenesis of diseases of invertebrates, including the suppression of diseases in beneficial species, and the use of diseases in controlling undesirable species. In addition, the journal publishes the results of physiological, morphological, genetic, immunological and ecological studies as related to the etiologic agents of diseases of invertebrates. The Journal of Invertebrate Pathology is the adopted journal of the Society for Invertebrate Pathology, and is available to SIP members at a special reduced price.
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