Antti-Jussi Ämmälä, Thomas P M Hancox, Fan Qiuyu, Alexandra Lahtinen, Sonja Sulkava, Victoria L Revell, Katrin Ackermann, Manfred Kayser, Debra J Skene, Tiina Paunio
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引用次数: 0
摘要
许多激素和基因表现出 24 小时的昼夜节律,这些节律也会受到睡眠不足的影响。在此,我们研究了在 24 小时睡眠/觉醒周期和随后 29 小时持续觉醒(1 晚睡眠剥夺)情况下 DNA 甲基化的昼夜节律。15 名健康男性(19-35 岁)在睡眠实验室度过了 3 天/夜:(1)适应期;(2)基线期;(3)完全剥夺睡眠的一天/夜。在基线期和完全睡眠剥夺期,每隔 3 小时收集一次外周血白细胞,连续收集 45 小时(从 15:00 开始),分析其 DNA 甲基化情况。表观基因组范围内的 DNA 甲基化变异通过 Infinium MethylationEPIC v2.0 Beadchip 试剂盒进行评估。对基线期和总睡眠剥夺期的时间序列数据分别进行了节律分析。还对同一时间点的昼间样本和睡眠剥夺样本进行了配对分析,以检测与睡眠剥夺有关的不同甲基化位点。在所有DNA甲基化位点中,有14%的位点在基线日/夜表现出甲基化的昼夜节律,而睡眠剥夺则改变了这一节律。在睡眠剥夺期间,不同甲基化位点的数量在睡眠剥夺期结束时有所增加,其中主要是低甲基化。在差异甲基化位置中,检测到与 FAS 免疫反应途径相关的基因富集。总之,DNA甲基化表现出昼夜节律性,在生物医学研究中将DNA甲基化作为生物标记物进行研究时,需要考虑这种昼夜变化。此外,在清醒状态下观察到的 DNA 甲基化变化可能是睡眠剥夺相关免疫反应改变的介质。
Daily rhythm in DNA methylation and the effect of total sleep deprivation.
Numerous hormones and genes exhibit diurnal 24-hr rhythms that can also be affected by sleep deprivation. Here we studied diurnal rhythms in DNA methylation under a 24-hr sleep/wake cycle and a subsequent 29 hr of continual wakefulness (1 night of sleep deprivation). Fifteen healthy men (19-35 years) spent 3 days/nights in a sleep laboratory: (1) adaptation; (2) baseline; (3) total sleep deprivation day/night. DNA methylation was analysed from peripheral blood leukocytes, collected every 3 hr for 45 hr (starting at 15:00 hours) during the baseline period and the total sleep deprivation period. Epigenome-wide DNA methylation variation was assessed with the Infinium MethylationEPIC v2.0 Beadchip kit. Rhythm analysis was performed separately for the baseline and the total sleep deprivation time-series data. Pairwise analysis between diurnal samples and sleep deprivation samples at the same timepoint was also carried out to detect differentially methylated positions related to sleep deprivation. Of all DNA methylation sites, 14% exhibited a diurnal rhythm in methylation on the baseline day/night that was altered by sleep deprivation. During sleep deprivation, the number of differentially methylated positions increased towards the end of the sleep deprivation period, with a dominating pattern of hypomethylation. Among differentially methylated positions, an enrichment of genes related to the FAS immune response pathway was detected. In conclusion, DNA methylation exhibits diurnal rhythmicity, and this time-of-day variation needs to be considered when studying DNA methylation as a biomarker in biomedical studies. In addition, the observed DNA methylation changes under wakefulness might serve as a mediator of sleep deprivation-related immune response alterations.
期刊介绍:
The Journal of Sleep Research is dedicated to basic and clinical sleep research. The Journal publishes original research papers and invited reviews in all areas of sleep research (including biological rhythms). The Journal aims to promote the exchange of ideas between basic and clinical sleep researchers coming from a wide range of backgrounds and disciplines. The Journal will achieve this by publishing papers which use multidisciplinary and novel approaches to answer important questions about sleep, as well as its disorders and the treatment thereof.