一家三级医院中因社区获得性下呼吸道感染而住院的儿童中的肺炎支原体。

IF 1.4 4区医学 Q4 IMMUNOLOGY

Indian Journal of Medical Microbiology Pub Date : 2025-01-01 DOI:10.1016/j.ijmmb.2024.100781

Surinder Kumar , Sanjeev R. Saigal , Sourabh Kumar , G.R. Sethi

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引用次数: 0

摘要

目的：在这项前瞻性研究中，采用血清学、巢式 PCR（聚合酶链反应）和多重 PCR 分析等方法调查了社区获得性下呼吸道感染（LRTI）患儿的肺炎双球菌感染率。在入院时和入院后四到六周抽取血样检测肺炎双球菌抗体。对鼻咽吸出物（NPA）进行巢式和多重 PCR 分析，以确定肺炎双球菌：结果：29名年龄小于5岁的儿童（74.35%）和10名年龄大于5岁的儿童（25.65%）感染了肺炎双球菌。肺炎链球菌与年龄的关系在 5 岁以下儿童中具有统计学意义[P=0.004]。男女儿童之间的差异无统计学意义[P=0.91]。临床和放射学结果与 M 型肺炎球菌感染无统计学意义上的显著相关性，但存在统计学意义上的浸润除外（P=0.04）。在 39 名（19.5%）儿童中，采用多重 PCR、巢式 PCR 和血清学相结合的方法发现了肺炎双球菌。以巢式 PCR 作为诊断标准时，血清学敏感性为 66.67%，特异性为 88.56%，阳性和阴性预测值分别为 36.36% 和 96.41%：总之，我们的研究确定了肺炎双球菌感染在社区获得性儿童 LRTI 中的关键作用，尤其是在五岁以下儿童中。喘息与急性肺炎双球菌感染有关。多重 PCR、巢式 PCR 和血清学可结合使用，帮助诊断社区获得性 LRTI 儿童的肺炎链球菌感染。

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Mycoplasma pneumoniae among children hospitalized in a tertiary hospital with community-acquired lower respiratory tract infections

Purpose

In this prospective study incidence of Mycoplasma pneumoniae (M. pneumoniae) infection was investigated in children with community-aquired lower respiratory tract infections (LRTIs) using methods such as serology, nested PCR (polymerase chain reaction), and multiplex PCR analysis.

Methods

M. pneumoniae infection was investigated among 200 children with LRTIs, ages 2 months to 12 years,. Blood samples were taken for M. pneumoniae antibodies both during admission and four to six weeks following enrollment. Nested and multiplex PCR analysis was performed to identify M. pneumoniae from nasopharyngeal aspirates (NPAs).

Results

Infection due to M. pneumoniae was noted in 29 children (74.35 %) < 5 years of age and in 10 children (25.65 %) aged ≥5 years. The relationship between M. pneumoniae and age was statistically significant in the age group of children under five years [P = 0.004]. No statistically significant difference was seen between male and female children [P = 0.91]. Clinical and radiological results and M pneumoniae infection did not demonstrate any statistically significant correlation with the exception of the presence of statistically significant infiltrates (P = 0.04). In 39 (19.5 %) children, M. pneumoniae was found using a combination of multiplex PCR, nested PCR, and serology. Serology sensitivity was 66.67 %, specificity was 88.56 %, positive and negative predictive values were 36.36 % and 96.41 %, respectively, when using nested PCR as the diagnostic criteria.

Conclusions

In summary, our research established the critical role of M. pneumoniae infection in community-acquired LRTIs in children particularly in children under five years. There was an association between wheezing and acute M. pneumoniae infection. Multiplex PCR, nested PCR, and serology are used in conjunction to help diagnose M. pneumoniae infection in children with community-acquired LRTIs.

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来源期刊

Indian Journal of Medical Microbiology IMMUNOLOGY-

CiteScore

2.20

自引率

0.00%

发文量

154

审稿时长

73 days

期刊介绍： Manuscripts of high standard in the form of original research, multicentric studies, meta analysis, are accepted. Current reports can be submitted as brief communications. Case reports must include review of current literature, clinical details, outcome and follow up. Letters to the editor must be a comment on or pertain to a manuscript already published in the IJMM or in relation to preliminary communication of a larger study. Review articles, Special Articles or Guest Editorials are accepted on invitation.