通过RNA-seq鉴定梨（P. communis 'Bartlett'）相关新型细胞病毒并确定其特征。

IF 2.3 3区生物学 Q3 MICROBIOLOGY

Current Microbiology Pub Date : 2024-12-16 DOI:10.1007/s00284-024-04010-2

Malyaj R Prajapati, Nitika Gupta, Pooja Thapa, Damini Diksha, Susheel Kumar Sharma, Virendra Kumar Baranwal

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引用次数: 0

摘要

利用转录组数据挖掘技术可以检测植物中的新型病毒。本研究通过对梨（P. communis 'Bartlett'）的RNA-seq数据的再分析，鉴定出一种新的细胞habdovirus——梨横纹病病毒1 （PRV-1）。PRV-1基因组RNA全长15628个核苷酸，包含6个开放阅读框（ORF），具有完整的编码区。同源性分析显示，PRV-1基因组序列与现有细胞habdovirus序列同源性为33.18 ~ 56.75%。基于基因组序列的系统发育分析表明，PRV-1与细胞habdov病毒属于同一进化支。根据序列划分标准，PRV-1是横纹肌病毒科细胞habdovirus属新发现的一种。梨中一个新病毒的鉴定将加深我们对植物细胞habdov多样性的认识。

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Identification and Characterization of a Novel Cytorhabdovirus Associated with Pear (P. communis 'Bartlett') Through RNA-seq.

Novel viruses in plants can be detected through transcriptome data mining. In this study, a novel cytorhabdovirus, pear rhabdovirus 1 (PRV-1) was identified through reanalysis of RNA-seq data of pear (P. communis 'Bartlett'). The genomic RNA of PRV-1, with complete coding region, measured 15,628 nucleotides (nts) and encompassed six open reading frames (ORF). Homology analysis of PRV-1 genome showed sequence identity of 33.18-56.75% with the existing cytorhabdovirus sequences. Phylogenetic analysis based on genome sequences showed that PRV-1 clustered in the same clade of cytorhabdoviruses. Based on the sequence demarcation criteria, PRV-1 represents a newly discovered species within the Cytorhabdovirus genus of the Rhabdoviridae family. Identification of a novel virus in pear will enhance our understanding on the diversity of plant cytorhabdoviruses.

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来源期刊

Current Microbiology 生物-微生物学

CiteScore

4.80

自引率

3.80%

发文量

380

审稿时长

2.5 months

期刊介绍： Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.