天竺葵内生菌 Diaporthe amygdali GWS39 的抗真菌、抗氧化活性和气相色谱-质谱分析。

IF 2.3 3区 生物学 Q3 MICROBIOLOGY
Misbah Majid, Bashir Ahmad Ganai, Abdul Hamid Wani
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引用次数: 0

摘要

内生真菌是天然产品的重要宝库。本研究调查了内生真菌 Diaporthe amygdali GWS39 的作用,这种真菌是从 Geranium wallichianum D. Don Ex Sweet 的气茎中分离出来的。Don Ex Sweet 的气生茎中分离出来的内生真菌 Diaporthe amygdali GWS39 的作用。通过显微镜、分子和系统发育技术证实,这种真菌内生菌与 Diaporthe amygdali 非常相似,具有良好的抗真菌和抗氧化能力。值得注意的是,本研究是全球首次报道 D. amygdali 是多年生草本植物 Geranium wallichianum D. Don Ex Sweet 的茎栖息内生真菌。Don Ex Sweet 的茎栖息内生菌。这项研究开创性地记录了内生菌 D. amygdali GWS39 对一些具有重要经济价值的病原体所表现出的广谱抗真菌活性。D. amygdali GWS39 粗提取物的抗氧化活性显示出很强的正相关性,甲醇提取物的 R2 值为 0.99,乙酸乙酯提取物的 R2 值为 0.93,表明其具有很高的抗氧化潜力。此外,本次研究可能是利用气相色谱-质谱(GC-MS)对内生菌 D. amygdali GWS39 的生物活性化学成分的首次记录。在乙酸乙酯提取物的气相色谱-质谱色谱图中,发现了环己胺、苯酚、2,6-二甲氧基苯磺酰胺、N-(2,6-二甲基苯基)-2-乙氧基-5-(四唑-1-基)、吗啉、1-, beta, -d-Ribofuranosyl-3-[5-tetraazolyl]-1,2,4, triazole。以前曾有报道称这些化合物具有很强的抗菌、抗真菌、抗氧化、抗病毒和抗癌活性。对甲醇粗提取物的分析发现,砷酸三(三甲基硅基)酯、正十六烷酸、10,11-十八烷二烯酸甲酯等化合物具有显著的抗生素、抗真菌、抗炎、抗病毒、抗组胺和抗癌活性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Antifungal, Antioxidant Activity, and GC-MS Profiling of Diaporthe amygdali GWS39: A First Report Endophyte from Geranium wallichianum.

Endophytic fungi serve as vital reservoirs of natural products. This study investigates the role of the endophytic fungus, Diaporthe amygdali GWS39, isolated from Geranium wallichianum D. Don Ex Sweet aerial stem. Showing a notable resemblance to Diaporthe amygdali, as confirmed through microscopic, molecular and phylogenetic techniques, this fungal endophyte displays promising antifungal and antioxidant capabilities. Remarkably, the present research marks the first report of D. amygdali as a stem inhabiting endophyte in an herbaceous perennial, Geranium wallichianum D. Don Ex Sweet on a global scale. This study pioneers the documentation of broad-spectrum antifungal activity exhibited by endophyte D. amygdali GWS39 against some economically important pathogens. The antioxidant activity of D. amygdali GWS39 crude extracts showed strong positive correlation, with R2 values of 0.99 for the methanolic extract and 0.93 for the ethyl acetate extract, indicating high antioxidant potential. In addition, the current investigation likely signifies the initial record of the bioactive chemical constituents of the endophyte D. amygdali GWS39 using GC-MS. In the GC-MS chromatogram of ethyl acetate extract, cyclohexaneamine, phenol, 2,6, dimethoxy-, benzenesulphonamide, N-(2,6, dimethylphenyl)-2-ethoxy-5-(tetrazol-1-yl), morpholine, 1-, beta, -d-Ribofuranosyl-3-[5-tetraazolyl]-1,2,4, triazole were identified. These compounds are previously reported for potent antibacterial, antifungal, antioxidant, antiviral, anticancer activities. The analysis of methanolic crude extract uncovers the presence of compounds such as arsenous acid tris(trimethylsilyl) ester, n-Hexadecanoic acid, methyl 10,11-octadecadienoate with noted antibiotic, antifungal, anti-inflammatory, antiviral, antihistaminic and anticancer activities.

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来源期刊
Current Microbiology
Current Microbiology 生物-微生物学
CiteScore
4.80
自引率
3.80%
发文量
380
审稿时长
2.5 months
期刊介绍: Current Microbiology is a well-established journal that publishes articles in all aspects of microbial cells and the interactions between the microorganisms, their hosts and the environment. Current Microbiology publishes original research articles, short communications, reviews and letters to the editor, spanning the following areas: physiology, biochemistry, genetics, genomics, biotechnology, ecology, evolution, morphology, taxonomy, diagnostic methods, medical and clinical microbiology and immunology as applied to microorganisms.
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