改善胰岛素抵抗大鼠的代谢和炎症状态:可可黄烷醇和亚极限有氧运动的急性干预可激活葡萄糖代谢的细胞内信号通路。

IF 6.6 2区 医学 Q1 NUTRITION & DIETETICS
B P Melo, A C Zacarias, J C C Oliveira, L M de Souza Cordeiro, N A C Horta, M O Poletini, C Tonoli, M L Dos Santos, S P Wanner, R Meeusen, E Heyman, G F Avelar, D D Soares
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引用次数: 0

摘要

引言:以高血糖为特征的 2 型糖尿病与肥胖和低度炎症密切相关:以高血糖为特征的 2 型糖尿病与肥胖和低度炎症密切相关。急性可可黄烷醇(CF)摄入对血管活性、认知功能和抗氧化酶活性有好处。然而,在患有代谢性疾病的人群中,可可黄烷醇在葡萄糖摄取、炎症介质方面的生理机制及其与有氧运动的相互作用仍不清楚:本研究旨在探讨 CF 单独或与急性有氧运动相结合对胰岛素抵抗(IR)大鼠肝脏、骨骼肌、胰腺和脂肪组织中葡萄糖摄取和炎症介质相关机制的急性影响:将 64 只 Wistar 大鼠(250 ± 10 克;15 周龄)置于普通饲料(CON)或由高脂肪饮食和富含果糖的饮料诱导的肥胖相关胰岛素抵抗(IR)状态下 30 天。在进行增量最大跑步机跑步测试 72 小时后,大鼠接受安慰剂溶液或 CF 补充剂(45 毫克-公斤-1 体重)。一小时后,大鼠要么休息,要么以峰值摄氧量(VO2peak)的 60% 在跑步机上跑步 30 分钟。实验结束后30分钟安乐死。在肝脏、胰腺、腓肠肌和附睾脂肪组织中使用酶联免疫吸附法测定炎症和抗炎细胞因子。肝脏和腓肠肌中的 TRB3 和 CPT1 mRNA 通过 q-RTPCR 进行评估,Akt 和 AMPK 磷酸化通过免疫组化进行检测:结果:CF可减轻IR大鼠亚极限有氧运动后的高血糖症状(p 结论:CF可减轻IR大鼠亚极限有氧运动后的高血糖症状:急性摄入 CF 并进行亚极限有氧运动可激活参与葡萄糖摄取和脂质代谢的关键蛋白和基因,改善炎症环境。这种协同效应可能有助于减轻与胰岛素抵抗有关的代谢并发症。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Enhancing metabolic and inflammatory status in insulin-resistant rats: Acute intervention with cocoa flavanols and submaximal aerobic exercise activates intracellular signaling pathways for glucose metabolism.

Introduction: Type 2 diabetes, characterized by hyperglycemia, is closely linked to obesity and low-grade inflammation. Acute cocoa flavanols (CF) intake has demonstrated benefits in vasoreactivity, cognitive functions, and antioxidant enzyme activity. However, the physiological mechanisms of CF concerning glucose uptake, inflammatory mediators, and their interplay with aerobic exercise remain unclear in populations with metabolic diseases.

Objective: This study aims to investigate the acute effects of CF, alone or combined with acute aerobic exercise on mechanisms involved in glucose uptake and inflammatory mediators in the liver, skeletal muscle, pancreas, and adipose tissue in insulin-resistant (IR) rats.

Methods: Sixty-four Wistar rats (250 ± 10g; 15 weeks age) were subjected to a regular chow (CON) or an obesity-associated insulin-resistant (IR) state induced by a high-fat diet and fructose-rich beverage for 30 days. Seventy-two hours after an incremental maximal treadmill running test, rats received a placebo solution or CF supplementation (45 mg·kg-1 of body weight). One hour later, they either rested or ran on a treadmill at 60 % of peak oxygen uptake (VO2peak) for 30 min. Euthanasia occurred 30 min post-experimental sessions. Inflammatory and anti-inflammatory cytokines were assayed using ELISA in the liver, pancreas, gastrocnemius muscle, and epididymal adipose tissues. TRB3 and CPT1 mRNA were assessed by q-RTPCR in the liver and gastrocnemius muscle while Akt and AMPK phosphorylation were examined by immunohistochemistry.

Results: CF attenuated hyperglycemia observed after submaximal aerobic exercise in IR rats (p < 0.001). In the liver, CF exhibited additive effects to aerobic exercise, enhancing Akt protein phosphorylation, potentially contributing to improved glucose uptake in IR rats. Submaximal aerobic exercise and CF increased AMPK protein phosphorylation in the liver (p < 0.001) and skeletal muscle (p < 0.001), reduced TRB3 gene expression (p < 0.01), elevated CPT-1a gene expression (p < 0.001), and ameliorated the inflammatory milieu in the pancreas, adipose tissue, liver, and gastrocnemius muscle.

Conclusion: Acute intake, of CF combined with submaximal aerobic exercise activates key proteins and genes involved in glucose uptake and lipid metabolism, improving the inflammatory milieu. This synergistic effect may contribute to mitigating metabolic complications associated with insulin resistance.

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来源期刊
Clinical nutrition
Clinical nutrition 医学-营养学
CiteScore
14.10
自引率
6.30%
发文量
356
审稿时长
28 days
期刊介绍: Clinical Nutrition, the official journal of ESPEN, The European Society for Clinical Nutrition and Metabolism, is an international journal providing essential scientific information on nutritional and metabolic care and the relationship between nutrition and disease both in the setting of basic science and clinical practice. Published bi-monthly, each issue combines original articles and reviews providing an invaluable reference for any specialist concerned with these fields.
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