Bahadir Yildiz, Jerome Jean-Gilles, Ellen J Giampoli, Sierra Kovar-Peltz, Qi Yang, Hiroshi Miyamoto, Ying Wang
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引用次数: 0
摘要
目的：将转移至胰腺的乳腺癌（BC）与原发性胰腺腺癌（PAC）区分开来至关重要，但却极具挑战性。乳腺癌与胰腺癌形态相似，免疫组化（IHC）特征也有重叠。我们研究了最近报道的毛细血管畸形综合征 1 型（TRPS1）IHC 在细针穿刺（FNA）标本中区分转移性 BC 和 PAC 的实用性：我们对连续的 PAC 患者（49 人）进行了评估。由于转移至胰腺的 BC 病例有限（3 例），因此纳入了转移至不同部位（包括淋巴结、肺、骨或软组织）的 BC 病例（23 例）。利用从 FNA 中获得的细胞块对 TRPS1 进行免疫组化。通过将阳性细胞的强度和百分比相乘，计算出 TRPS1 表达的定量分数。免疫反应评分分为阴性、低度阳性、中度阳性或高度阳性：在 49 例 PAC 中，47 例（95.9%）表现为阴性，2 例（4.1%）表现为 TRPS1 低阳性。然而，在26例转移性BC病例中，有23例（88.0%）TRPS1表达为高阳性，包括12例三阴性BC（TNBC）病例中的10例（83.3%）和14例非TNBC病例中的13例（92.9%）：我们的研究结果表明，TRPS1 IHC 是区分转移性 BC 和 PAC 的一种高度准确可靠的方法。
TRPS1 is a useful marker in differentiating metastatic breast carcinoma from pancreatic adenocarcinoma in fine-needle aspiration specimens.
Objectives: Distinction of metastatic breast carcinoma (BC) to the pancreas from primary pancreatic adenocarcinoma (PAC) is essential but challenging. Breast carcinoma shares similar morphology and exhibits an overlapping immunohistochemistry (IHC) profile with PAC. We investigated the utility of recently reported trichorhinophalangeal syndrome type 1 (TRPS1) IHC in differentiating metastatic BC from PAC in fine-needle aspiration (FNA) specimens.
Methods: We assessed consecutive patients of PAC (n = 49). Due to limited cases of metastatic BC to the pancreas (n = 3), cases of metastatic BC (n = 23) in various locations, including lymph node, lung, bone, or soft tissue, were included. Immunohistochemistry for TRPS1 was performed by using the cell blocks obtained from FNA. A quantitative score for TRPS1 expression was calculated by multiplying the intensity and the percentage of positive cells. Immunoreactivity scores were assigned as negative, low positive, intermediate positive, or high positive.
Results: In 49 PAC cases, 47 (95.9%) exhibited negative while 2 (4.1%) exhibited low positive TRPS1 expression. However, TRPS1 expression was high positive in 23 (88.0%) of 26 metastatic BC cases, including 10 (83.3%) of 12 triple-negative BC (TNBC) and 13 (92.9%) of 14 non-TNBC cases.
Conclusions: Our results suggest that TRPS1 IHC represents a highly accurate and reliable method for differentiating metastatic BC from PAC.
期刊介绍：
The American Journal of Clinical Pathology (AJCP) is the official journal of the American Society for Clinical Pathology and the Academy of Clinical Laboratory Physicians and Scientists. It is a leading international journal for publication of articles concerning novel anatomic pathology and laboratory medicine observations on human disease. AJCP emphasizes articles that focus on the application of evolving technologies for the diagnosis and characterization of diseases and conditions, as well as those that have a direct link toward improving patient care.
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