Proteomic and spectral analysis reveals the role of extracellular polymeric substances in mercury biosorption by activated sludge under high-altitude conditions.

In high-altitude regions, elevated mercury (Hg) levels in wastewater treatment plants (WWTPs) influent raise concerns about treatment efficiency and environmental impact. This study investigated the Hg biosorption capacity of activated sludge under high-altitude conditions, focusing on the binding mechanisms between EPS and Hg, and variations in EPS secretion. Low pressure, oxygen, and temperature at high altitudes increase EPS secretion, enhancing Hg biosorption. EPS provides numerous binding sites for Hg, forming nonfluorescent complexes with tryptophan-like and aromatic proteins, while hydrocarbon and oxygen-containing groups limit Hg entry into microbial cells. Proteomic analysis revealed the upregulation of transporters, stress-resistance, and binding proteins, along with those involved in carbon and amino acid metabolism, which enhance microbial resilience and EPS production, leading to increased Hg biosorption. These insights reveal adaptive mechanisms that optimize pollutant removal in high-altitude environments.