IF 8 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Junjie Wang, Zhuowei Cheng, Yunfei Su, Jiade Wang, Dongzhi Chen, Jianmeng Chen, Xiaoming Wu, Aobo Chen, Zhenyu Gu
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引用次数: 0

摘要

本研究的重点是有毒气体 H2S 和温室气体 CO2 的生物强化吸收和生物脱硫耦合过程。结果表明,在碱性溶液稳定吸收 H2S 和 CO2(第一阶段)的基础上,在吸收塔中加入气升生物反应器工艺溶液可增强对它们的吸收(第二阶段)。具体来说,当 H₂S 和 CO₂ 的入口浓度分别为 3% (30,000 ppmv) 和 30% (300,000 ppmv) 时,出口气体主要为 H₂S、CO₂ 和 N₂。出口 H2S 和 CO2 浓度分别从第一阶段的 10,038 ± 1166 ppmv 和 49,897 ± 2545 ppmv 降至第二阶段的 940 ± 163 ppmv 和 21,000 ± 2165 ppmv。后续生物反应器的 S0 生成性能(348 ± 20-503 ± 23 mg S/L)和生物量浓度(467 ± 13-677 ± 55 mg/L)也随着 H2S 和 CO2 吸收的增强而提高。生物对 H2S 和 CO2 吸收的增强不同于物理化学因素,因为它取决于几个生理参数,如微生物群落组成和基因表达水平。在本研究中,硫自养反硝化细菌 Thioalkalivibrio 和 Arenimonas 的丰度和活性较高(丰度:69.5% 和 21.1%,表达量:82.4% 和 13.9%),它们是该系统中 H2S 和 CO2 生物强化吸收的主要贡献者。此外,硫化物:醌氧化还原酶(SQR,编码基因 sqr)的高表达(百万分之 45 ± 9 至 821 ± 102 转录本)也可能是 H2S 吸收增强的主要因素。更多 H2S 的氧化产生了更多能量,从而提高了核酮糖-1,5-二磷酸羧化酶/加氧酶(Rubisco,编码基因 rbcLS)的羧化活性,从而增强了对 CO2 的吸收。增强对 H2S 的吸收可提高对 CO2 的吸收并促进微生物的生长,这反过来又有利于 H2S 的新陈代谢,形成一种互补的生物增强吸收。这项研究提供了一种新的策略,证明了自养型硫化物氧化微生物在同时去除 H₂S 和同化 CO₂ 方面的潜力,并加深了对其基本机制的理解。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Metagenomics and metatranscriptomics insights into microbial enhancement of H2S removal and CO2 assimilation.

This study focuses on the coupled process of bio-enhanced absorption and biodesulfurization for the toxic gas H2S and the greenhouse gas CO2. The results show that on the basis of stabilized absorption of H2S and CO2 by alkaline solution (Stage I), the addition of air-lift bioreactor process solution in the absorption column enhanced their absorption (Stage II). Specifically, at constant inlet concentrations of H₂S and CO₂ of 3% (30,000 ppmv) and 30% (300,000 ppmv), respectively, the outlet gases were primarily H₂S, CO₂, and N₂. And the outlet H2S and CO2 concentrations decreased from 10,038 ± 1166 ppmv and 49,897 ± 2545 ppmv in Stage I to 940 ± 163 ppmv and 21,000 ± 2165 ppmv in Stage II. S0-producing performance (348 ± 20-503 ± 23 mg S/L) and biomass concentration (467 ± 13-677 ± 55 mg/L) in the subsequent bioreactor also increased in response to the enhanced absorption of H2S and CO2. Biologically enhanced H2S and CO2 absorption differs from physicochemical factors in that it depends on several physiological parameters such as microbial community composition and gene expression levels. In this study, the sulfur autotrophic denitrifying bacteria Thioalkalivibrio and Arenimonas had high abundance and activity (abundance: 69.5% and 21.1%, expression: 82.4% and 13.9%), and they were the main contributors to the bio-enhanced absorption of H2S and CO2 in this system. In addition, the main factor for enhanced H2S absorption could be the high expression of sulfide:quinone oxidoreductase (SQR, encoding gene sqr) (45 ± 9 to 821 ± 102 transcripts per million). Enhanced CO2 absorption could have been achieved by the oxidation of more H2S generating more energy to increase the carboxylation activity of ribulose-1,5-bisphosphate carboxylase/oxygenase (Rubisco, encoding genes rbcLS). Enhanced H2S absorption enhances CO2 absorption and facilitates microbial growth, which in turn benefits the metabolism of H2S, creating a complementary biologically enhanced absorption. This study provides a novel strategy, demonstrating the potential of autotrophic sulfide-oxidizing microorganisms in the simultaneous removal of H₂S and assimilation of CO₂, and offers a deeper understanding of the underlying mechanisms.

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来源期刊
Journal of Environmental Management
Journal of Environmental Management 环境科学-环境科学
CiteScore
13.70
自引率
5.70%
发文量
2477
审稿时长
84 days
期刊介绍: The Journal of Environmental Management is a journal for the publication of peer reviewed, original research for all aspects of management and the managed use of the environment, both natural and man-made.Critical review articles are also welcome; submission of these is strongly encouraged.
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