IF 1.7 4区 医学 Q3 MEDICINE, RESEARCH & EXPERIMENTAL
American journal of translational research Pub Date : 2024-11-25 eCollection Date: 2024-01-01 DOI:10.62347/VXHU6738
Lixiang Li, Xiaoyong Niu, Wanzong Zhang, Ziyu Wang, Shengming Zhang, Qi Zhang, Wei Qi, Wenshan Jing, Gaobin Hu, Long Huang, Ziyi Li, Qiyu Feng, Hui Peng, Qingsheng Yu
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引用次数: 0

摘要

简介肝胆管结石(HL)是一种复杂的肝胆疾病,具有复发率高的特点。本研究旨在评估复方中药十二味芎归颗粒(TSLG)治疗 HL 的疗效,并探讨其潜在机制:方法:对157例确诊为HL的患者进行回顾性分析,将其分为两组:对照组和研究组。对照组术后不进行任何治疗,而研究组术后口服 TSLG,每天三次,持续两个月。两组患者分别在术后 1 个月、2 个月和 3 个月接受电话随访。手术前后测量了肝功能指标,并使用高通量测序(HTS)分析了 miRNA 表达谱。此外,还通过 Western 印迹评估了相关蛋白质的表达水平:结果:与对照组相比,研究组术后肝功能指标明显降低(P < 0.05)。此外,有64个miRNA在HL患者中有差异表达。对这64个miRNA的进一步分析表明,它们异常地靶向Hippo信号通路。进一步的实验结果表明,TAZ蛋白在HL患者中表达升高,反映了这些患者体内Hippo信号通路的异常激活。TSLG 治疗明显降低了 YAP、TAZ 和 SREBP-2 蛋白的表达,同时增加了 p-YAP 和 p-TAZ 蛋白的表达(均 P < 0.05)。此外,TSLG 还能抑制 LPS 诱导的 WRL68 细胞的细胞外酸化率(ECAR):TSLG通过下调固醇调节元件结合蛋白-2(SREBP-2)和抑制Hippo信号通路,有效改善了术后肝功能。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Twelve Shugan Lidan Granules from traditional Chinese medicine can improve liver function in patients with postoperative hepatolithiasis by inhibiting the Hippo signaling pathway.

Introduction: Hepatolithiasis (HL) is a complex liver and biliary disorder characterized by high rates of recurrence. This study aimed to evaluate the efficacy of Twelve Shugan Lidan Granules (TSLG), a compound herbal traditional Chinese formulation, in the treatment of HL, as well as to investigate its underlying mechanism.

Methods: A retrospective analysis was conducted involving 157 patients diagnosed with HL, who were divided into two groups: the control group and the research group. In the control group, no treatment was given postoperatively, while in the research group, TSLG was orally administered three times a day postoperatively for two months. Both groups were followed up by telephone at 1 month, 2 months, and 3 months postoperatively. Liver function indicators were measured before and after surgery, and miRNA expression profiling was analyzed using high-throughput sequencing (HTS). Additionally, the expression levels of related proteins were assessed through western blots.

Results: Postoperative liver function indicators were significantly lower in the research group compared to the control group (P < 0.05). Additionally, 64 miRNAs were differentially expressed in HL patients. Further analysis of 64 miRNAs revealed their abnormal targeting of the Hippo signaling pathway. Further experimental results indicate that TAZ protein expression is elevated in HL patients, reflecting abnormal activation of the Hippo signaling pathway in these patients. TSLG treatment significantly reduced the expression of YAP, TAZ, and SREBP-2 proteins, while increasing the expression of p-YAP and p-TAZ proteins (all P < 0.05). Furthermore, TSLG inhibited the Extracellular Acidification Rate (ECAR) in LPS-induced WRL68 cells.

Conclusion: TSLG effectively improved postoperative liver function by downregulating sterol regulatory element-binding protein-2 (SREBP-2) and inhibiting the Hippo signaling pathway.

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American journal of translational research
American journal of translational research ONCOLOGY-MEDICINE, RESEARCH & EXPERIMENTAL
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552
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