使用树枝状介孔二氧化硅纳米颗粒和金纳米颗粒对心肌肌钙蛋白 I 进行定量和快速横向流动免疫测定。

IF 2.7 3区 化学 Q2 CHEMISTRY, ANALYTICAL
Yafei Li, Yu Yao, Qingqing Hua and Jishun Li
{"title":"使用树枝状介孔二氧化硅纳米颗粒和金纳米颗粒对心肌肌钙蛋白 I 进行定量和快速横向流动免疫测定。","authors":"Yafei Li, Yu Yao, Qingqing Hua and Jishun Li","doi":"10.1039/D4AY02060J","DOIUrl":null,"url":null,"abstract":"<p >Acute myocardial infarction (AMI) is considered to be one of the predominant causes of human death; therefore, a rapid and accurate diagnostic method for AMI is urgently required. In this work, a highly sensitive lateral flow immunoassay (LFIA) platform was designed and fabricated for the quantitative determination of cardiac troponin I (cTnI) using a scanner, a smartphone and a colloidal gold immunoassay analyzer. To overcome the limitation of low sensitivity of traditional colloidal gold-based LFIA, three-dimensionally assembled gold nanoparticles (AuNPs) within a dendritic mesoporous silica nanoparticle (DMSN) scaffold were fabricated as signal labels. The assembly structure greatly enhanced the light extinction ability of a single label for signal amplification. The DMSNs@Au-based LFIA strips exhibited excellent detection performance including a high sensitivity (LOD = 70 pg mL<small><sup>−1</sup></small>) and wide linear range (0.5–40 ng mL<small><sup>−1</sup></small>) and precision with good specificity. The successful determination of cTnI by the test strips provides the ability to diagnose AMI at an early stage and expands the diagnostic window of AMI, while also having advantages such as low cost and user-friendliness. Therefore, we believe that the test strips fabricated in this work have great potential to be applied for practical clinical applications for the early and accurate diagnosis of AMI.</p>","PeriodicalId":64,"journal":{"name":"Analytical Methods","volume":" 4","pages":" 698-707"},"PeriodicalIF":2.7000,"publicationDate":"2024-12-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Quantitative and rapid lateral flow immunoassay for cardiac troponin I using dendritic mesoporous silica nanoparticles and gold nanoparticles†\",\"authors\":\"Yafei Li, Yu Yao, Qingqing Hua and Jishun Li\",\"doi\":\"10.1039/D4AY02060J\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p >Acute myocardial infarction (AMI) is considered to be one of the predominant causes of human death; therefore, a rapid and accurate diagnostic method for AMI is urgently required. In this work, a highly sensitive lateral flow immunoassay (LFIA) platform was designed and fabricated for the quantitative determination of cardiac troponin I (cTnI) using a scanner, a smartphone and a colloidal gold immunoassay analyzer. To overcome the limitation of low sensitivity of traditional colloidal gold-based LFIA, three-dimensionally assembled gold nanoparticles (AuNPs) within a dendritic mesoporous silica nanoparticle (DMSN) scaffold were fabricated as signal labels. The assembly structure greatly enhanced the light extinction ability of a single label for signal amplification. The DMSNs@Au-based LFIA strips exhibited excellent detection performance including a high sensitivity (LOD = 70 pg mL<small><sup>−1</sup></small>) and wide linear range (0.5–40 ng mL<small><sup>−1</sup></small>) and precision with good specificity. The successful determination of cTnI by the test strips provides the ability to diagnose AMI at an early stage and expands the diagnostic window of AMI, while also having advantages such as low cost and user-friendliness. Therefore, we believe that the test strips fabricated in this work have great potential to be applied for practical clinical applications for the early and accurate diagnosis of AMI.</p>\",\"PeriodicalId\":64,\"journal\":{\"name\":\"Analytical Methods\",\"volume\":\" 4\",\"pages\":\" 698-707\"},\"PeriodicalIF\":2.7000,\"publicationDate\":\"2024-12-03\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Analytical Methods\",\"FirstCategoryId\":\"92\",\"ListUrlMain\":\"https://pubs.rsc.org/en/content/articlelanding/2025/ay/d4ay02060j\",\"RegionNum\":3,\"RegionCategory\":\"化学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q2\",\"JCRName\":\"CHEMISTRY, ANALYTICAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Analytical Methods","FirstCategoryId":"92","ListUrlMain":"https://pubs.rsc.org/en/content/articlelanding/2025/ay/d4ay02060j","RegionNum":3,"RegionCategory":"化学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q2","JCRName":"CHEMISTRY, ANALYTICAL","Score":null,"Total":0}
引用次数: 0

摘要

急性心肌梗死(AMI)被认为是人类死亡的主要原因之一;因此,迫切需要一种快速准确的AMI诊断方法。在这项工作中,设计并制造了一个高灵敏度的侧流免疫分析(LFIA)平台,用于使用扫描仪,智能手机和胶体金免疫分析分析仪定量测定心肌肌钙蛋白I (cTnI)。为了克服传统胶体金基LFIA灵敏度低的局限性,在树突状介孔二氧化硅纳米颗粒(DMSN)支架内制备三维组装金纳米颗粒(AuNPs)作为信号标记。这种组装结构极大地增强了单个标签的消光能力,用于信号放大。DMSNs@Au-based LFIA试纸具有灵敏度高(LOD = 70 pg mL-1)、线性范围宽(0.5 ~ 40 ng mL-1)、精密度高、特异度好等特点。cTnI试纸的成功测定为AMI的早期诊断提供了可能,扩大了AMI的诊断窗口,同时具有成本低、使用方便等优点。因此,我们认为本工作制作的试纸具有很大的临床应用潜力,可用于AMI的早期准确诊断。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Quantitative and rapid lateral flow immunoassay for cardiac troponin I using dendritic mesoporous silica nanoparticles and gold nanoparticles†

Quantitative and rapid lateral flow immunoassay for cardiac troponin I using dendritic mesoporous silica nanoparticles and gold nanoparticles†

Acute myocardial infarction (AMI) is considered to be one of the predominant causes of human death; therefore, a rapid and accurate diagnostic method for AMI is urgently required. In this work, a highly sensitive lateral flow immunoassay (LFIA) platform was designed and fabricated for the quantitative determination of cardiac troponin I (cTnI) using a scanner, a smartphone and a colloidal gold immunoassay analyzer. To overcome the limitation of low sensitivity of traditional colloidal gold-based LFIA, three-dimensionally assembled gold nanoparticles (AuNPs) within a dendritic mesoporous silica nanoparticle (DMSN) scaffold were fabricated as signal labels. The assembly structure greatly enhanced the light extinction ability of a single label for signal amplification. The DMSNs@Au-based LFIA strips exhibited excellent detection performance including a high sensitivity (LOD = 70 pg mL−1) and wide linear range (0.5–40 ng mL−1) and precision with good specificity. The successful determination of cTnI by the test strips provides the ability to diagnose AMI at an early stage and expands the diagnostic window of AMI, while also having advantages such as low cost and user-friendliness. Therefore, we believe that the test strips fabricated in this work have great potential to be applied for practical clinical applications for the early and accurate diagnosis of AMI.

求助全文
通过发布文献求助,成功后即可免费获取论文全文。 去求助
来源期刊
Analytical Methods
Analytical Methods CHEMISTRY, ANALYTICAL-FOOD SCIENCE & TECHNOLOGY
CiteScore
5.10
自引率
3.20%
发文量
569
审稿时长
1.8 months
期刊介绍: Early applied demonstrations of new analytical methods with clear societal impact
×
引用
GB/T 7714-2015
复制
MLA
复制
APA
复制
导出至
BibTeX EndNote RefMan NoteFirst NoteExpress
×
提示
您的信息不完整,为了账户安全,请先补充。
现在去补充
×
提示
您因"违规操作"
具体请查看互助需知
我知道了
×
提示
确定
请完成安全验证×
copy
已复制链接
快去分享给好友吧!
我知道了
右上角分享
点击右上角分享
0
联系我们:info@booksci.cn Book学术提供免费学术资源搜索服务,方便国内外学者检索中英文文献。致力于提供最便捷和优质的服务体验。 Copyright © 2023 布克学术 All rights reserved.
京ICP备2023020795号-1
ghs 京公网安备 11010802042870号
Book学术文献互助
Book学术文献互助群
群 号:481959085
Book学术官方微信