基于石墨烯量子点-纳米多孔金复合材料的无标记电化学生物传感器，用于高灵敏度检测胶质瘤细胞

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2025-02-01 DOI:10.1016/j.aca.2024.343555

Xiaohui Wang , Xinran Li , Baoquan Wang , Yanxia Xie , Xueru Cui , Jin Jiao , Jianwei Jiao , Yachao Zhang

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引用次数: 0

摘要

神经胶质瘤占所有恶性原发性脑肿瘤的80%，死亡率高。组织病理学检查是目前胶质瘤的诊断方法，但其侵入性手术干预可引起脑水肿或损害神经功能。液体活检被证明是一种有效的胶质瘤检测方法。然而，血脑屏障限制了血液中循环肿瘤细胞（CTCs）的数量，这对胶质瘤CTCs的敏感检测提出了挑战。本研究旨在利用纳米复合材料的独特特性和Angiopep2 （Ang-2）的特异性，开发一种能够灵敏识别胶质瘤ctc的方法。结果以石墨烯量子点(GQDs)-纳米孔金（NPG）纳米复合材料为固定平台，以Ang-2蛋白为生物识别元件，成功构建了一种新型的无标记阻抗生物传感器，用于检测胶质瘤ctc。将GQDs均匀组装到NPG上，制备出具有独特结构和功能特性的新型GQDs-NPG纳米复合材料。由于GQDs-NPG纳米复合材料的高电子传递效率，所开发的生物传感器的检测范围为1 ~ 1×106细胞mL-1，最小检测限为1细胞mL-1。此外，胶质瘤细胞生物传感器对多种细胞系具有较强的抗干扰能力，储存21天后生物传感器的稳定性保持在96%。此外，胶质瘤细胞生物传感器在人血清样品中检测到的胶质瘤细胞数量与添加到样品中的标准值具有出色的一致性。研究提供了一种新的GQDs-NPG纳米复合材料，并首次开发了基于GQDs-NPG的神经胶质瘤ctc检测电化学生物传感器。该胶质瘤细胞生物传感器在复杂的生物基质中具有灵敏度高、检测限低、抗干扰能力强、稳定性好的特点。成功实现了胶质瘤细胞在人血清中的可靠检测，为胶质瘤ctc的液体活检鉴定和胶质瘤疾病的早期诊断提供了一个很好的选择。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A label-free electrochemical biosensor based on graphene quantum dots-nanoporous gold nanocomposite for highly sensitive detection of glioma cell

查看原文本刊更多论文

A label-free electrochemical biosensor based on graphene quantum dots-nanoporous gold nanocomposite for highly sensitive detection of glioma cell

Background

Glioma accounts for 80 % of all malignant primary brain tumors with a high mortality rate. Histopathological examination is the current diagnostic methods for glioma, but its invasive surgical interventions can cause cerebral edema or impair neural functioning. Liquid biopsy proves to be an efficient method for glioma detection. However, the blood-brain barrier restricts the number of circulating tumor cells (CTCs) in the bloodstream, posing a challenge for sensitive detection of glioma CTCs. This study aims to use the unique characteristics of nanocomposites and the specificity of Angiopep2 (Ang-2) to develop a method that can sensitively identify glioma CTCs.

Results

Herein, a novel label-free impedimetric biosensor was successfully constructed for glioma CTCs detection by using graphene quantum dots (GQDs)-nanoporous gold (NPG) nanocomposites as the immobilized platform and the Ang-2 protein as biorecognition element. The GQDs was homogeneously assembled onto NPG, resulting in the creation of a novel GQDs-NPG nanocomposite with unique structure and function properties. Due to the high electron transfer efficiency of the GQDs-NPG nanocomposite, the developed biosensor exhibited a wild detection range from 1 to 1 × 10⁶ cell mL⁻¹, with a minimal detection limit of 1 cell mL⁻¹. Additionally, the glioma cell biosensor demonstrated a strong anti-interference ability against multiple cell lines, and the stability of the biosensor remained at 96 % after 21 days of storage. Besides, the quantities of glioma cells detected in human serum samples by the glioma cell biosensor demonstrated outstanding consistency with the standard values added to the samples.

Significance

The study provided a novel GQDs-NPG nanocomposite and an electrochemical biosensor based on GQDs-NPG was firstly developed for glioma CTCs detection. The glioma cell biosensor showed high sensitivity, low detection limit, strong anti-interference ability, and good stability in complex biological matrix. The reliable detection of glioma cell was successfully realized in human serum, providing an excellent option for liquid biopsy of glioma CTCs identification and early diagnosis of glioma diseases.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.