基于石墨烯量子点-纳米多孔金复合材料的无标记电化学生物传感器，用于高灵敏度检测胶质瘤细胞

IF 5.7 2区化学 Q1 CHEMISTRY, ANALYTICAL

Analytica Chimica Acta Pub Date : 2024-12-16 DOI:10.1016/j.aca.2024.343555

Xiaohui Wang, Xinran Li, Baoquan Wang, Yanxia Xie, Xueru Cui, Jin Jiao, Jianwei Jiao, Yachao Zhang

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摘要

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A label-free electrochemical biosensor based on graphene quantum dots-nanoporous gold nanocomposite for highly sensitive detection of glioma cell

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A label-free electrochemical biosensor based on graphene quantum dots-nanoporous gold nanocomposite for highly sensitive detection of glioma cell

Background

Glioma accounts for 80% of all malignant primary brain tumors with a high mortality rate. Histopathological examination is the current diagnostic methods for glioma, but its invasive surgical interventions can cause cerebral edema or impair neural functioning. Liquid biopsy proves to be an efficient method for glioma detection. However, the blood-brain barrier restricts the number of circulating tumor cells (CTCs) in the bloodstream, posing a challenge for sensitive detection of glioma CTCs. This study aims to use the unique characteristics of nanocomposites and the specificity of Angiopep2 (Ang-2) to develop a method that can sensitively identify glioma CTCs.

Results

Herein, a novel label-free impedimetric biosensor was successfully constructed for glioma CTCs detection by using graphene quantum dots (GQDs)-nanoporous gold (NPG) nanocomposites as the immobilized platform and the Ang-2 protein as biorecognition element. The GQDs was homogeneously assembled onto NPG, resulting in the creation of a novel GQDs-NPG nanocomposite with unique structure and function properties. Due to the high electron transfer efficiency of the GQDs-NPG nanocomposite, the developed biosensor exhibited a wild detection range from 1 to 1×10⁶ cell mL^-1, with a minimal detection limit of 1 cell mL^-1. Additionally, the glioma cell biosensor demonstrated a strong anti-interference ability against multiple cell lines, and the stability of the biosensor remained at 96% after 21 days of storage. Besides, the quantities of glioma cells detected in human serum samples by the glioma cell biosensor demonstrated outstanding consistency with the standard values added to the samples.

Significance

The study provided a novel GQDs-NPG nanocomposite and an electrochemical biosensor based on GQDs-NPG was firstly developed for glioma CTCs detection. The glioma cell biosensor showed high sensitivity, low detection limit, strong anti-interference ability, and good stability in complex biological matrix. The reliable detection of glioma cell was successfully realized in human serum, providing an excellent option for liquid biopsy of glioma CTCs identification and early diagnosis of glioma diseases.

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来源期刊

Analytica Chimica Acta 化学-分析化学

CiteScore

10.40

自引率

6.50%

发文量

1081

审稿时长

38 days

期刊介绍： Analytica Chimica Acta has an open access mirror journal Analytica Chimica Acta: X, sharing the same aims and scope, editorial team, submission system and rigorous peer review. Analytica Chimica Acta provides a forum for the rapid publication of original research, and critical, comprehensive reviews dealing with all aspects of fundamental and applied modern analytical chemistry. The journal welcomes the submission of research papers which report studies concerning the development of new and significant analytical methodologies. In determining the suitability of submitted articles for publication, particular scrutiny will be placed on the degree of novelty and impact of the research and the extent to which it adds to the existing body of knowledge in analytical chemistry.