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IF 10.1 1区生物学 Q1 BIOTECHNOLOGY & APPLIED MICROBIOLOGY

Plant Biotechnology Journal Pub Date : 2024-12-16 DOI:10.1111/pbi.14547

Xin Liu, Feng Zhang, Ziqi Xun, Jiale Shao, Wenfan Luo, Xiaokang Jiang, Jiachang Wang, Jian Wang, Shuai Li, Qibing Lin, Yulong Ren, Huixian Zhao, Zhijun Cheng, Jianmin Wan

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引用次数: 0

摘要

分蘖是决定水稻产量的重要因素。目前，研究主要集中在低阶分蘖（LOT）的生长上，而高阶分蘖（HOT）生长的调控机制尚不清楚。本研究发现，OsNL1与OsTOPLESS2之间存在相互作用，这种相互作用是由OsNL1的HAN结构域中的9个氨基酸VDCTLSLGT核心基序介导的。toppless2 突变体表现出与 nl1 相似的 HOT 数量增加。通过ChIP-seq分析，我们发现OsNL1招募OsTOPLESS2在OsMOC1和OsMOC3的启动子中进行组蛋白去乙酰化，从而调控HOT的生长。此外，我们还发现，HAN结构域对于OsNL1作为抑制因子的功能至关重要。总之，我们的研究揭示了水稻 HOT 外生长的部分机制，并解读了 HAN 结构域的分子生物学功能。这将有助于全面了解分蘖的生长和含HAN结构域基因的作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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The OsNL1-OsTOPLESS2-OsMOC1/3 pathway regulates high-order tiller outgrowth in rice

Tiller is an important factor in determining rice yield. Currently, researches mainly focus on the outgrowth of low-order tiller (LOT), while the regulation mechanism of high-order tiller (HOT) outgrowth has remained unknown. In this study, we detected one OsNL1 mutant, nl1, exhibiting HOT numbers increase, and found that OsNL1 interacts with OsTOPLESS2, which was mediated by the core motif of nine amino acids VDCTLSLGT within the HAN domain of OsNL1. The topless2 mutant exhibits similar HOT number increase as in the nl1. Through ChIP-seq analysis, we revealed that OsNL1 recruits OsTOPLESS2 to conduct histone deacetylation in the promoters of OsMOC1 and OsMOC3 to regulate HOT outgrowth. Moreover, we showed that the HAN domain is essential for OsNL1 function as a repressor. In summary, our study reveals partial mechanism of HOT outgrowth in rice and deciphers the molecular biology function of the HAN domain. This will contribute to the comprehensive understanding of tiller outgrowth and the role of HAN-domain-containing genes.

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来源期刊

Plant Biotechnology Journal 生物-生物工程与应用微生物

CiteScore

20.50

自引率

2.90%

发文量

201

审稿时长

1 months

期刊介绍： Plant Biotechnology Journal aspires to publish original research and insightful reviews of high impact, authored by prominent researchers in applied plant science. The journal places a special emphasis on molecular plant sciences and their practical applications through plant biotechnology. Our goal is to establish a platform for showcasing significant advances in the field, encompassing curiosity-driven studies with potential applications, strategic research in plant biotechnology, scientific analysis of crucial issues for the beneficial utilization of plant sciences, and assessments of the performance of plant biotechnology products in practical applications.