Bo Li, Shuang Zhang, Xiaoxian Yun, Chengyi Liu, Rui Xiao, Mingjie Lu, Xiaomei Xu, Fuwei Lin
{"title":"NEDD4 对大鼠骨间充质干细胞成骨潜能的影响与 PI3K/Akt 通路有关。","authors":"Bo Li, Shuang Zhang, Xiaoxian Yun, Chengyi Liu, Rui Xiao, Mingjie Lu, Xiaomei Xu, Fuwei Lin","doi":"10.1016/j.diff.2024.100830","DOIUrl":null,"url":null,"abstract":"<p><p>Neural precursor cell expressed developmentally down-regulated 4 (NEDD4) is an E3 ubiquitin ligase implicated in craniofacial development. Emerging evidence suggests that NEDD4 may down-regulates Akt signaling, a key element of the PI3K/Akt pathway involved in cell differentiation. This study aimed to investigate NEDD4's role in bone mesenchymal stem cells (BMSCs) differentiation and its interaction with the PI3K/Akt pathway. BMSCs were isolated from SD rats, and NEDD4 expression increased during osteogenic differentiation. Silencing NEDD4 with siRNA elevated alkaline phosphatase (ALP), osteocalcin (OCN), Akt, and mTORC1 expression during induction, while subsequent treatment with LY294002 (a broad spectrum PI3K inhibitor) reduced Akt, mTORC1, ALP, and OCN levels. These findings suggest that NEDD4 may inhibit BMSCs differentiation by suppressing the PI3K/Akt pathway during osteogenesis.</p>","PeriodicalId":50579,"journal":{"name":"Differentiation","volume":"141 ","pages":"100830"},"PeriodicalIF":2.2000,"publicationDate":"2024-12-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"NEDD4's effect on osteoblastogenesis potential of bone mesenchymal stem cells in rats concerned with PI3K/Akt pathway.\",\"authors\":\"Bo Li, Shuang Zhang, Xiaoxian Yun, Chengyi Liu, Rui Xiao, Mingjie Lu, Xiaomei Xu, Fuwei Lin\",\"doi\":\"10.1016/j.diff.2024.100830\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>Neural precursor cell expressed developmentally down-regulated 4 (NEDD4) is an E3 ubiquitin ligase implicated in craniofacial development. Emerging evidence suggests that NEDD4 may down-regulates Akt signaling, a key element of the PI3K/Akt pathway involved in cell differentiation. This study aimed to investigate NEDD4's role in bone mesenchymal stem cells (BMSCs) differentiation and its interaction with the PI3K/Akt pathway. BMSCs were isolated from SD rats, and NEDD4 expression increased during osteogenic differentiation. Silencing NEDD4 with siRNA elevated alkaline phosphatase (ALP), osteocalcin (OCN), Akt, and mTORC1 expression during induction, while subsequent treatment with LY294002 (a broad spectrum PI3K inhibitor) reduced Akt, mTORC1, ALP, and OCN levels. These findings suggest that NEDD4 may inhibit BMSCs differentiation by suppressing the PI3K/Akt pathway during osteogenesis.</p>\",\"PeriodicalId\":50579,\"journal\":{\"name\":\"Differentiation\",\"volume\":\"141 \",\"pages\":\"100830\"},\"PeriodicalIF\":2.2000,\"publicationDate\":\"2024-12-10\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Differentiation\",\"FirstCategoryId\":\"99\",\"ListUrlMain\":\"https://doi.org/10.1016/j.diff.2024.100830\",\"RegionNum\":3,\"RegionCategory\":\"生物学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q4\",\"JCRName\":\"CELL BIOLOGY\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Differentiation","FirstCategoryId":"99","ListUrlMain":"https://doi.org/10.1016/j.diff.2024.100830","RegionNum":3,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q4","JCRName":"CELL BIOLOGY","Score":null,"Total":0}
NEDD4's effect on osteoblastogenesis potential of bone mesenchymal stem cells in rats concerned with PI3K/Akt pathway.
Neural precursor cell expressed developmentally down-regulated 4 (NEDD4) is an E3 ubiquitin ligase implicated in craniofacial development. Emerging evidence suggests that NEDD4 may down-regulates Akt signaling, a key element of the PI3K/Akt pathway involved in cell differentiation. This study aimed to investigate NEDD4's role in bone mesenchymal stem cells (BMSCs) differentiation and its interaction with the PI3K/Akt pathway. BMSCs were isolated from SD rats, and NEDD4 expression increased during osteogenic differentiation. Silencing NEDD4 with siRNA elevated alkaline phosphatase (ALP), osteocalcin (OCN), Akt, and mTORC1 expression during induction, while subsequent treatment with LY294002 (a broad spectrum PI3K inhibitor) reduced Akt, mTORC1, ALP, and OCN levels. These findings suggest that NEDD4 may inhibit BMSCs differentiation by suppressing the PI3K/Akt pathway during osteogenesis.
期刊介绍：
Differentiation is a multidisciplinary journal dealing with topics relating to cell differentiation, development, cellular structure and function, and cancer. Differentiation of eukaryotes at the molecular level and the use of transgenic and targeted mutagenesis approaches to problems of differentiation are of particular interest to the journal.
The journal will publish full-length articles containing original work in any of these areas. We will also publish reviews and commentaries on topics of current interest.
The principal subject areas the journal covers are: • embryonic patterning and organogenesis
• human development and congenital malformation
• mechanisms of cell lineage commitment
• tissue homeostasis and oncogenic transformation
• establishment of cellular polarity
• stem cell differentiation
• cell reprogramming mechanisms
• stability of the differentiated state
• cell and tissue interactions in vivo and in vitro
• signal transduction pathways in development and differentiation
• carcinogenesis and cancer
• mechanisms involved in cell growth and division especially relating to cancer
• differentiation in regeneration and ageing
• therapeutic applications of differentiation processes.
求助内容：
应助结果提醒方式：
京公网安备 11010802042870号
