将血小板储存在小袋中作为全尺寸容器中血小板功能的模型。

IF 2.5 3区 医学 Q2 HEMATOLOGY
Transfusion Pub Date : 2025-01-01 Epub Date: 2024-12-14 DOI:10.1111/trf.18097
Kathleen Kelly, Tamir Kanias, Caroline Leite, Crystal Stanley, Larry J Dumont
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引用次数: 0

摘要

背景:评价添加溶液、储存容器、新的收集和储存方法以及其他潜在的改进是资源密集型的,导致血小板从血库库存中转移,并且需要花费大量时间来完成研究招募。我们的目标是评估小袋用于血小板储存研究的可行性,并通过使用标准化的呼吸测定试验,将每日代谢能力与在改变pH值、燃料和最终产物的动态储存环境中观察到的结果分开。方法:100%血浆中采集的单供者单采血小板经小体积去除以满足二次处理要求。将小体积(23±1.4 mL)放入由血小板储存材料制成的50 mL袋中,保存7天,并通过一组体外检测进行评估。用呼吸计测定血小板生物能量(耗氧量和产酸率)。结果:血小板pH值下降、活化和凝血酶生成的模式与历史报道一致。第7天,乳酸生成率(54.1±11.3 μmol/1012plt/h)与pH降低、活化增加和凝血酶生成能力显著相关。呼吸测量显示糖酵解能力的降低和ATP生产超过储存对氧化系统的累积损伤。讨论:小包呈现出代谢变化和激活的存储概况,与满袋存储的历史数据一致。因此,该系统有望为血小板储存实验提供一个平台,以最大化研究环境中收集的血小板,并且不影响本研究中患者治疗的可用性。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Platelet storage in small bags as a model of platelet function in full-sized containers.

Background: Evaluation of additive solutions, storage containers, new collection and storage methods, and other potential modifications is resource intensive, resulting in diversion of platelets away from blood bank inventories and significant time to complete study recruitments. Our goal was to evaluate the feasibility of a small bag for the study of platelet storage, and, by using a standardized respirometry test, separate daily metabolic capacity from observations made in the dynamic storage environment of changing pH, fuels, and end products.

Methods: Single-donor apheresis platelets collected in 100% plasma had small volumes removed to meet secondary processing requirements. Small volumes (23 ± 1.4 mL) were placed in 50-mL bags constructed of platelet storage material, stored 7 days, and assessed with a panel of in vitro assays. Platelet bioenergetics (oxygen consumption and acid production rates) were measured with a respirometer.

Results: The patterns of platelet pH decline, activation, and potency by thrombin generation were consistent with historical reports. Lactate production rates (54.1 ± 11.3 μmol/1012plt/h) were significantly correlated with pH decrease, increased activation, and thrombin generation potency by Day 7. Respirometry revealed a reduction of the glycolytic capacity and accumulating damage to the oxidative system for ATP production over storage.

Discussion: Small bags present a storage profile of metabolic changes and activation consistent with historical data for full bag storage. Therefore, this system has promise to provide a platform for scaling experiments of platelet storage in a manner that maximizes platelets collected in research settings and does not compromise availability for patient treatment as exercised in this study.

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来源期刊
Transfusion
Transfusion 医学-血液学
CiteScore
4.70
自引率
20.70%
发文量
426
审稿时长
1 months
期刊介绍: TRANSFUSION is the foremost publication in the world for new information regarding transfusion medicine. Written by and for members of AABB and other health-care workers, TRANSFUSION reports on the latest technical advances, discusses opposing viewpoints regarding controversial issues, and presents key conference proceedings. In addition to blood banking and transfusion medicine topics, TRANSFUSION presents submissions concerning patient blood management, tissue transplantation and hematopoietic, cellular, and gene therapies.
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