用水凝胶覆盖纳米通道的外表面，以防止蛋白质干扰离子流。

IF 5.6 1区化学 Q1 CHEMISTRY, ANALYTICAL

Talanta Pub Date : 2024-12-09 DOI:10.1016/j.talanta.2024.127367

Xueting Sun , Xiaojin Zhang , Tingting Cao, Suqing Huang, Yu Dai, Fan Xia

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引用次数: 0

摘要

纳米孔/纳米通道传感在化学和生物学领域是一种很有前途的分析方法。然而，由于复杂样品中非分析物的干扰，通过纳米孔/纳米通道直接分析未预处理的样品仍然是一个很大的挑战。本文报道了一种用氧化石墨烯/海藻酸钙（GCA）杂化水凝胶覆盖阳极氧化铝（AAO）圆柱状纳米通道多孔膜的非均质膜，以减少蛋白质对电流检测信号的干扰。水凝胶层通过位阻阻断蛋白质，使其不会堵塞和影响通道。结果表明，当GCA水凝胶覆盖在AAO膜的外表面时，蛋白质对离子电流的干扰从52%降低到5%。我们的工作为减少复杂样品中非分析物的干扰提供了一种策略，并证明了水凝胶对纳米孔/纳米通道传感的抗干扰功能。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Outer-surface covering of nanochannels with hydrogel for anti-protein-interference on the ion current

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Outer-surface covering of nanochannels with hydrogel for anti-protein-interference on the ion current

Nanopore/nanochannel sensing is a promising analytical method in the fields of chemistry and biology. However, due to the interference of non-analytes in complex samples, directly analyzing un-pretreated samples through nanopores/nanochannels remains a great challenge. Here, we report a type of heterogeneous membrane by covering anodic aluminum oxide (AAO) cylindrical nanochannel porous membrane with graphene oxide/calcium alginate (GCA) hybrid hydrogel to reduce the interference of protein on the current detection signal. The hydrogel layer blocks the protein through steric hindrance, so that it will not block and affect the channel. The results show that when the GCA hydrogel is covered on the outer surface of AAO membrane, the interference of protein on the ion current decreases from 52 % to 5 %. Our work provides a strategy for reducing the interference of non-analytes in complex samples, and demonstrates the anti-interference function of hydrogels on nanopore/nanochannel sensing.

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来源期刊

Talanta 化学-分析化学

CiteScore

12.30

自引率

4.90%

发文量

861

审稿时长

29 days

期刊介绍： Talanta provides a forum for the publication of original research papers, short communications, and critical reviews in all branches of pure and applied analytical chemistry. Papers are evaluated based on established guidelines, including the fundamental nature of the study, scientific novelty, substantial improvement or advantage over existing technology or methods, and demonstrated analytical applicability. Original research papers on fundamental studies, and on novel sensor and instrumentation developments, are encouraged. Novel or improved applications in areas such as clinical and biological chemistry, environmental analysis, geochemistry, materials science and engineering, and analytical platforms for omics development are welcome. Analytical performance of methods should be determined, including interference and matrix effects, and methods should be validated by comparison with a standard method, or analysis of a certified reference material. Simple spiking recoveries may not be sufficient. The developed method should especially comprise information on selectivity, sensitivity, detection limits, accuracy, and reliability. However, applying official validation or robustness studies to a routine method or technique does not necessarily constitute novelty. Proper statistical treatment of the data should be provided. Relevant literature should be cited, including related publications by the authors, and authors should discuss how their proposed methodology compares with previously reported methods.