Sandile S Msimango, Mahmoud Nasr, Faizal Bux, Sheena Kumari
{"title":"化学需氧量与氮比率对上流式厌氧污泥毯反应器中 ANAMMOX 细菌生长的影响。","authors":"Sandile S Msimango, Mahmoud Nasr, Faizal Bux, Sheena Kumari","doi":"10.2166/wst.2024.376","DOIUrl":null,"url":null,"abstract":"<p><p>While several studies have investigated the effect of varying carbon-to-nitrogen (C/N) ratios on the ANAMMOX performance, there is still a research gap in illustrating the shift in 16S rRNA gene copy number and functional microbial population during operation. Hence, this study focuses on utilizing a reference gene and target functional genes to demonstrate the synergetic interaction between ANAMMOX, ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB), using an up-flow anaerobic sludge blanket (UASB) under different C/N conditions. It was demonstrated that elevating the C/N ratio from 1.0 to 2.0 reduced the COD and NH4<sup>+</sup>-N removal efficiencies from 80.12 to 48.62% and from 88.99 to 72.59%, respectively. Based on the qPCR evaluation, at the C/N ratio of 1.5, the abundance of ANAMMOX, AOB, <i>Nitrobacter</i>, and <i>Nitrospira</i> was 2.52 × 10<sup>6</sup>, 82, 5.39 × 10<sup>3</sup>, and 12.98 × 10<sup>3</sup> copies/μL, respectively. However, with the further increase of C/N ratio to 2.0, their abundance was reduced to 1.09 × 10<sup>6</sup>, 46, 0.98 × 10<sup>3</sup>, and 3.47 × 10<sup>3</sup> copies/μL, respectively. The expression of <i>hzo</i> gene encoding for hydrazine dehydrogenase was 169-folds at C/N = 1 and almost inhibited at C/N = 2. The results of microbial population structure using 16S rRNA reverse transcriptase (RT)-qPCR technique depicted a competition between ANAMMOX and heterotrophic bacteria for the available substrate at higher C/N ratios.</p>","PeriodicalId":23653,"journal":{"name":"Water Science and Technology","volume":"90 11","pages":"2978-2990"},"PeriodicalIF":2.5000,"publicationDate":"2024-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Impact of chemical oxygen demand to nitrogen ratio on ANAMMOX bacterial growth in an up-flow anaerobic sludge blanket reactor.\",\"authors\":\"Sandile S Msimango, Mahmoud Nasr, Faizal Bux, Sheena Kumari\",\"doi\":\"10.2166/wst.2024.376\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<p><p>While several studies have investigated the effect of varying carbon-to-nitrogen (C/N) ratios on the ANAMMOX performance, there is still a research gap in illustrating the shift in 16S rRNA gene copy number and functional microbial population during operation. Hence, this study focuses on utilizing a reference gene and target functional genes to demonstrate the synergetic interaction between ANAMMOX, ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB), using an up-flow anaerobic sludge blanket (UASB) under different C/N conditions. It was demonstrated that elevating the C/N ratio from 1.0 to 2.0 reduced the COD and NH4<sup>+</sup>-N removal efficiencies from 80.12 to 48.62% and from 88.99 to 72.59%, respectively. Based on the qPCR evaluation, at the C/N ratio of 1.5, the abundance of ANAMMOX, AOB, <i>Nitrobacter</i>, and <i>Nitrospira</i> was 2.52 × 10<sup>6</sup>, 82, 5.39 × 10<sup>3</sup>, and 12.98 × 10<sup>3</sup> copies/μL, respectively. However, with the further increase of C/N ratio to 2.0, their abundance was reduced to 1.09 × 10<sup>6</sup>, 46, 0.98 × 10<sup>3</sup>, and 3.47 × 10<sup>3</sup> copies/μL, respectively. The expression of <i>hzo</i> gene encoding for hydrazine dehydrogenase was 169-folds at C/N = 1 and almost inhibited at C/N = 2. The results of microbial population structure using 16S rRNA reverse transcriptase (RT)-qPCR technique depicted a competition between ANAMMOX and heterotrophic bacteria for the available substrate at higher C/N ratios.</p>\",\"PeriodicalId\":23653,\"journal\":{\"name\":\"Water Science and Technology\",\"volume\":\"90 11\",\"pages\":\"2978-2990\"},\"PeriodicalIF\":2.5000,\"publicationDate\":\"2024-12-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Water Science and Technology\",\"FirstCategoryId\":\"93\",\"ListUrlMain\":\"https://doi.org/10.2166/wst.2024.376\",\"RegionNum\":4,\"RegionCategory\":\"环境科学与生态学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"2024/11/15 0:00:00\",\"PubModel\":\"Epub\",\"JCR\":\"Q3\",\"JCRName\":\"ENGINEERING, ENVIRONMENTAL\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Water Science and Technology","FirstCategoryId":"93","ListUrlMain":"https://doi.org/10.2166/wst.2024.376","RegionNum":4,"RegionCategory":"环境科学与生态学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2024/11/15 0:00:00","PubModel":"Epub","JCR":"Q3","JCRName":"ENGINEERING, ENVIRONMENTAL","Score":null,"Total":0}
Impact of chemical oxygen demand to nitrogen ratio on ANAMMOX bacterial growth in an up-flow anaerobic sludge blanket reactor.
While several studies have investigated the effect of varying carbon-to-nitrogen (C/N) ratios on the ANAMMOX performance, there is still a research gap in illustrating the shift in 16S rRNA gene copy number and functional microbial population during operation. Hence, this study focuses on utilizing a reference gene and target functional genes to demonstrate the synergetic interaction between ANAMMOX, ammonia-oxidizing bacteria (AOB), and nitrite-oxidizing bacteria (NOB), using an up-flow anaerobic sludge blanket (UASB) under different C/N conditions. It was demonstrated that elevating the C/N ratio from 1.0 to 2.0 reduced the COD and NH4+-N removal efficiencies from 80.12 to 48.62% and from 88.99 to 72.59%, respectively. Based on the qPCR evaluation, at the C/N ratio of 1.5, the abundance of ANAMMOX, AOB, Nitrobacter, and Nitrospira was 2.52 × 106, 82, 5.39 × 103, and 12.98 × 103 copies/μL, respectively. However, with the further increase of C/N ratio to 2.0, their abundance was reduced to 1.09 × 106, 46, 0.98 × 103, and 3.47 × 103 copies/μL, respectively. The expression of hzo gene encoding for hydrazine dehydrogenase was 169-folds at C/N = 1 and almost inhibited at C/N = 2. The results of microbial population structure using 16S rRNA reverse transcriptase (RT)-qPCR technique depicted a competition between ANAMMOX and heterotrophic bacteria for the available substrate at higher C/N ratios.
期刊介绍:
Water Science and Technology publishes peer-reviewed papers on all aspects of the science and technology of water and wastewater. Papers are selected by a rigorous peer review procedure with the aim of rapid and wide dissemination of research results, development and application of new techniques, and related managerial and policy issues. Scientists, engineers, consultants, managers and policy-makers will find this journal essential as a permanent record of progress of research activities and their practical applications.