金纳米颗粒的选择性表面钝化：一种在侧流免疫分析中提高灵敏度的策略

IF 13.2 1区工程技术 Q1 ENGINEERING, CHEMICAL

Chemical Engineering Journal Pub Date : 2025-01-01 DOI:10.1016/j.cej.2024.158571

Zongyou Chen , Sha Yu , Shijin Huang , Wei Zhang , Ping Guo , Juan Peng , Yonghua Xiong , Bingfeng Ma , Weihua Lai

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引用次数: 0

摘要

金纳米颗粒（AuNPs）被广泛认为是侧流免疫分析（LFIA）的主要信号标记。由于aunp的亮度不足，很难在靶标浓度发生微小变化的情况下产生可区分的信号变化，严重影响了LFIA的检测灵敏度。在本研究中，为了提高LFIA的检测灵敏度，我们合成了支化金纳米颗粒（BGNPs）作为吸收主导的信号标签。采用表面阻滞剂对不同枝密度的BGNPs进行微调，使其在金纳米颗粒表面成核，实现部分钝化，促进枝结构的生长。系统地探讨了BGNPs的光学性质及其对LFIA检测灵敏度的影响。并比较了BGNPs-LFIA与AuNPs-LFIA的检测灵敏度。结果表明，高密度分支BGNPs具有极弱的散射和极强的吸收特性，有利于LFIA中吸收主导的信号输出。因此，基于高密度分支BGNPs构建BGNPs- lfia，可有效提高黄瓜、辣椒、草莓、苹果中多菌灵（CBZ）的检测灵敏度。BGNPs-LFIA检测CBZ的截止值和检出限分别为50 ng mL−1和0.14ng mL−1，分别比AuNPs-LFIA低4倍（200 ng mL−1）和8.79倍（1.23 ng mL−1）。BGNPs-LFIA的回收率为83.83 % ~ 113.26 %，变异系数为2.29 % ~ 14.16 %。此外，BGNPs-LFIA的定量结果与液相色谱-串联质谱（LC-MS/MS）和酶联免疫吸附法（ELISA）的定量结果具有较高的一致性。研究结果表明，BGNPs是一种很有前途的LFIA信号标签，为LFIA信号标签的选择提供了有价值的参考。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

Selective surface passivation of gold nanoparticles: A strategy for enhanced sensitivity in lateral flow immunoassay

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Selective surface passivation of gold nanoparticles: A strategy for enhanced sensitivity in lateral flow immunoassay

Gold nanoparticles (AuNPs) are widely recognized as the predominant signal label in lateral flow immunoassay (LFIA). Due to the inadequate brightness of AuNPs, it is challenging to generate distinguishable signal changes with slight variations of target concentration, thereby severely hindering the detection sensitivity of LFIA. In this study, to improve the detection sensitivity of LFIA, we synthesized branched gold nanoparticles (BGNPs) as an absorption-dominated signal label. BGNPs with different branch densities were finely tuned by a surface blocker agent, which nucleated at the surface of the gold nanoparticles for partial passivation to promote the growth of the branch structure. The optical properties of BGNPs, as well as their impact on the detection sensitivity of LFIA, were systematically explored. Additionally, the detection sensitivity of BGNPs-LFIA was further compared with that of AuNPs-LFIA. The results showed that high-density branch BGNPs exhibited excellent optical properties with very weak scattering and extremely strong absorption, which favored the absorption-dominated signal output in LFIA. Thus, BGNPs-LFIA was developed based on high-density branch BGNPs to effectively improve sensitivity for the detection of carbendazim （CBZ） in cucumber, chili, strawberry, and apple. The cut-off value and the limit of detection of BGNPs-LFIA for the detection of CBZ were 50 ng mL⁻¹ and 0.14 ng mL⁻¹, respectively, which were 4-fold (200 ng mL⁻¹) and 8.79-fold (1.23 ng mL⁻¹) lower than those of AuNPs-LFIA. The recoveries of BGNPs-LFIA were 83.83 %–113.26 %, with coefficients of variation of 2.29 %–14.16 %. Furthermore, the quantitative results of BGNPs-LFIA showed high consistency with those of liquid chromatography-tandem mass spectrometry (LC-MS/MS) and enzyme-linked immunosorbent assay (ELISA). Our study demonstrates that BGNPs are promising signal labels in LFIA and provide valuable references for selecting appropriate signal labels in LFIA.

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来源期刊

Chemical Engineering Journal 工程技术-工程：化工

CiteScore

21.70

自引率

9.30%

发文量

6781

审稿时长

2.4 months

期刊介绍： The Chemical Engineering Journal is an international research journal that invites contributions of original and novel fundamental research. It aims to provide an international platform for presenting original fundamental research, interpretative reviews, and discussions on new developments in chemical engineering. The journal welcomes papers that describe novel theory and its practical application, as well as those that demonstrate the transfer of techniques from other disciplines. It also welcomes reports on carefully conducted experimental work that is soundly interpreted. The main focus of the journal is on original and rigorous research results that have broad significance. The Catalysis section within the Chemical Engineering Journal focuses specifically on Experimental and Theoretical studies in the fields of heterogeneous catalysis, molecular catalysis, and biocatalysis. These studies have industrial impact on various sectors such as chemicals, energy, materials, foods, healthcare, and environmental protection.