一种用于药物性肝损伤中羟基自由基成像的荧光探针。

IF 4.3 2区化学 Q1 SPECTROSCOPY

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy Pub Date : 2024-12-09 DOI:10.1016/j.saa.2024.125569

Mengshan Yu , Hanyue Xiang , Ayijiang Taledaohan , Wenzhuo Fu , Hongyu Li , Yuji Wang , Nao Xiao

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引用次数: 0

摘要

羟自由基是活性氧（ROS）中氧化性最强的一种，与人体健康密切相关。本研究报告了一种新型高灵敏度氢氧自由基开启荧光探针 BIJ-H。BIJ-H 通过三步反应合成，只需用贫溶剂洗涤即可获得纯品。在含有 BIJ-H 的纯水溶液中，加入 OH 后，探针被氧化成 BIJ，在 550 纳米波长的激发下，在 625 纳米波长处产生荧光发射。这种传感行为可在生理 pH 条件下实现。BIJ-H 对 OH 的检测限低至 0.1379 μM。由于 BIJ-H 具有良好的分析特性，该探针已被应用于 HepG2 细胞中 OH 的荧光成像以及对乙酰氨基酚诱导的急性肝损伤。BIJ-H 探针有望用于生理或病理过程中 OH 的定性和定量检测。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

A turn-on fluorescent probe for imaging of hydroxyl radicals in drug-induced liver injury

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A turn-on fluorescent probe for imaging of hydroxyl radicals in drug-induced liver injury

Hydroxyl radical is the most oxidizing type of reactive oxygen species (ROS), which is closely related to body health. In this work, a novel turn-on fluorescent probe BIJ-H for OH with high sensitivity was reported. BIJ-H was synthesized by a three-step reaction, and the pure product was obtained by only washing with poor solvents. In a neat aqueous solution containing BIJ-H, after adding OH, the probe was oxidized to BIJ, which produced fluorescence emission at 625 nm under the excitation with 550 nm. This sensing behavior can be achieved under physiological pH conditions. The detection limit of BIJ-H for OH was as low as 0.1379 μM. Thanks to the good analytical properties of BIJ-H, the probe has been applied to fluorescence imaging of OH in HepG2 cells and in acute liver injury induced by acetaminophen. The probe BIJ-H is expected to be used in the qualitative and quantitative detection of OH in physiological or pathological processes.

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来源期刊

Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy 物理-光谱学

CiteScore

8.40

自引率

11.40%

发文量

1364

审稿时长

40 days

期刊介绍： Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy (SAA) is an interdisciplinary journal which spans from basic to applied aspects of optical spectroscopy in chemistry, medicine, biology, and materials science. The journal publishes original scientific papers that feature high-quality spectroscopic data and analysis. From the broad range of optical spectroscopies, the emphasis is on electronic, vibrational or rotational spectra of molecules, rather than on spectroscopy based on magnetic moments. Criteria for publication in SAA are novelty, uniqueness, and outstanding quality. Routine applications of spectroscopic techniques and computational methods are not appropriate. Topics of particular interest of Spectrochimica Acta Part A include, but are not limited to: Spectroscopy and dynamics of bioanalytical, biomedical, environmental, and atmospheric sciences, Novel experimental techniques or instrumentation for molecular spectroscopy, Novel theoretical and computational methods, Novel applications in photochemistry and photobiology, Novel interpretational approaches as well as advances in data analysis based on electronic or vibrational spectroscopy.