Cost-efficient analysis of cannabinoids in therapeutic oils using HPLC with UV and mass spectrometry detection.

Cannabis oil, derived from Cannabis sativa plants, is increasingly used for therapeutic purposes across a wide range of diseases. Accurate quantification of cannabinoids is essential, especially for cannabis products sourced from informal markets where supply origins are uncertain. This study aimed to develop a cost-effective, robust analytical methodology using liquid chromatography in combination with UV- and mass detectors for the quantification of key cannabinoids (THC, CBD and CBN) and the identification of THCA and CBDA. Utilising an isocratic flow, the method achieved effective separation within 17 min, ensuring simplicity and reproducibility. The methodology validation was aligned with ICH guidelines' requirements for selectivity, linearity, precision, accuracy, and matrix effects. Successful application of this method to both homemade and commercial cannabis oil samples underscores its relevance for adjusting therapeutic doses and optimising CBD:THC ratios for specific disease treatments.