Ping Qiu , Jiahui Sun , Jiahao Liu , Zhichao Mei , Chaochun Wang , Xin Wang , Qinqin Chen , Xin He , Xinghua Xiong , Linfeng Bao , Zhongsong Liu , Honghong Wu , Yunlong Zhai , Lunwen Qian
{"title":"甘草-枸杞衍生纳米材料通过激活ja介导的免疫反应增强油菜菌核菌抗茎腐病能力","authors":"Ping Qiu , Jiahui Sun , Jiahao Liu , Zhichao Mei , Chaochun Wang , Xin Wang , Qinqin Chen , Xin He , Xinghua Xiong , Linfeng Bao , Zhongsong Liu , Honghong Wu , Yunlong Zhai , Lunwen Qian","doi":"10.1016/j.indcrop.2024.120279","DOIUrl":null,"url":null,"abstract":"<div><div>Due to the lack of resistant germplasm, sclerotinia stem rot (SSR) caused by <em>S. sclerotiorum</em> (<em>Sclerotinia sclerotiorum</em>) has become the most destructive disease in rapeseed (<em>Brassica napus</em> L.) production. Nanobiotechnology has recently been considered as a sustainable approach for crop protection by reducing pesticide use. In this study, a novel blue-green fluorescent nanocomplex derived from licorice and wolfberry (LW-NCs) was synthesized and characterized, exhibiting stronger antioxidant activity compared with licorice or wolfberry extracts alone. LW-NCs promoted the growth of rapeseed seedlings without affecting photosynthesis efficiency. <em>In vitro</em> antifungal assays revealed that LW-NCs significantly inhibited the growth of <em>S. sclerotiorum</em> at a concentration of 200 ppm. Transcriptomic and metabolomic analyses demonstrated that foliar application of LW-NCs increased the JA content and the activities of SOD and CAT in rapeseed, ultimately enhancing the tolerance of rapeseed to <em>S. sclerotiorum</em>. In addition, toxicity experiments have demonstrated that LW-NCs possess excellent biocompatibility. These results indicated that the application of LW-NCs can not only improve the disease resistance of rapeseed, but also help reduce the harm of pesticides to human health, ensuring the safety of them for agricultural applications.</div></div>","PeriodicalId":13581,"journal":{"name":"Industrial Crops and Products","volume":"224 ","pages":"Article 120279"},"PeriodicalIF":6.2000,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":"0","resultStr":"{\"title\":\"Licorice-wolfberry derived nanomaterials enhance sclerotinia stem rot resistance by activating JA-mediated immune response in rapeseed\",\"authors\":\"Ping Qiu , Jiahui Sun , Jiahao Liu , Zhichao Mei , Chaochun Wang , Xin Wang , Qinqin Chen , Xin He , Xinghua Xiong , Linfeng Bao , Zhongsong Liu , Honghong Wu , Yunlong Zhai , Lunwen Qian\",\"doi\":\"10.1016/j.indcrop.2024.120279\",\"DOIUrl\":null,\"url\":null,\"abstract\":\"<div><div>Due to the lack of resistant germplasm, sclerotinia stem rot (SSR) caused by <em>S. sclerotiorum</em> (<em>Sclerotinia sclerotiorum</em>) has become the most destructive disease in rapeseed (<em>Brassica napus</em> L.) production. Nanobiotechnology has recently been considered as a sustainable approach for crop protection by reducing pesticide use. In this study, a novel blue-green fluorescent nanocomplex derived from licorice and wolfberry (LW-NCs) was synthesized and characterized, exhibiting stronger antioxidant activity compared with licorice or wolfberry extracts alone. LW-NCs promoted the growth of rapeseed seedlings without affecting photosynthesis efficiency. <em>In vitro</em> antifungal assays revealed that LW-NCs significantly inhibited the growth of <em>S. sclerotiorum</em> at a concentration of 200 ppm. Transcriptomic and metabolomic analyses demonstrated that foliar application of LW-NCs increased the JA content and the activities of SOD and CAT in rapeseed, ultimately enhancing the tolerance of rapeseed to <em>S. sclerotiorum</em>. In addition, toxicity experiments have demonstrated that LW-NCs possess excellent biocompatibility. These results indicated that the application of LW-NCs can not only improve the disease resistance of rapeseed, but also help reduce the harm of pesticides to human health, ensuring the safety of them for agricultural applications.</div></div>\",\"PeriodicalId\":13581,\"journal\":{\"name\":\"Industrial Crops and Products\",\"volume\":\"224 \",\"pages\":\"Article 120279\"},\"PeriodicalIF\":6.2000,\"publicationDate\":\"2025-02-01\",\"publicationTypes\":\"Journal Article\",\"fieldsOfStudy\":null,\"isOpenAccess\":false,\"openAccessPdf\":\"\",\"citationCount\":\"0\",\"resultStr\":null,\"platform\":\"Semanticscholar\",\"paperid\":null,\"PeriodicalName\":\"Industrial Crops and Products\",\"FirstCategoryId\":\"97\",\"ListUrlMain\":\"https://www.sciencedirect.com/science/article/pii/S0926669024022568\",\"RegionNum\":1,\"RegionCategory\":\"农林科学\",\"ArticlePicture\":[],\"TitleCN\":null,\"AbstractTextCN\":null,\"PMCID\":null,\"EPubDate\":\"\",\"PubModel\":\"\",\"JCR\":\"Q1\",\"JCRName\":\"AGRICULTURAL ENGINEERING\",\"Score\":null,\"Total\":0}","platform":"Semanticscholar","paperid":null,"PeriodicalName":"Industrial Crops and Products","FirstCategoryId":"97","ListUrlMain":"https://www.sciencedirect.com/science/article/pii/S0926669024022568","RegionNum":1,"RegionCategory":"农林科学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"","PubModel":"","JCR":"Q1","JCRName":"AGRICULTURAL ENGINEERING","Score":null,"Total":0}
Licorice-wolfberry derived nanomaterials enhance sclerotinia stem rot resistance by activating JA-mediated immune response in rapeseed
Due to the lack of resistant germplasm, sclerotinia stem rot (SSR) caused by S. sclerotiorum (Sclerotinia sclerotiorum) has become the most destructive disease in rapeseed (Brassica napus L.) production. Nanobiotechnology has recently been considered as a sustainable approach for crop protection by reducing pesticide use. In this study, a novel blue-green fluorescent nanocomplex derived from licorice and wolfberry (LW-NCs) was synthesized and characterized, exhibiting stronger antioxidant activity compared with licorice or wolfberry extracts alone. LW-NCs promoted the growth of rapeseed seedlings without affecting photosynthesis efficiency. In vitro antifungal assays revealed that LW-NCs significantly inhibited the growth of S. sclerotiorum at a concentration of 200 ppm. Transcriptomic and metabolomic analyses demonstrated that foliar application of LW-NCs increased the JA content and the activities of SOD and CAT in rapeseed, ultimately enhancing the tolerance of rapeseed to S. sclerotiorum. In addition, toxicity experiments have demonstrated that LW-NCs possess excellent biocompatibility. These results indicated that the application of LW-NCs can not only improve the disease resistance of rapeseed, but also help reduce the harm of pesticides to human health, ensuring the safety of them for agricultural applications.
期刊介绍:
Industrial Crops and Products is an International Journal publishing academic and industrial research on industrial (defined as non-food/non-feed) crops and products. Papers concern both crop-oriented and bio-based materials from crops-oriented research, and should be of interest to an international audience, hypothesis driven, and where comparisons are made statistics performed.