D K Parasana, I H Kalyani, A V Kachchhi, P G Koringa, P M Makwana, D R Patel, U V Ramani, B B Javia, S N Ghodasara
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引用次数: 0
摘要
假中间葡萄球菌被认为是犬脓皮病最常见的病因。抗菌药耐药性被认为是一个全球公共卫生问题。本研究的主要目的是利用全基因组测序技术评估伪中间葡萄球菌分离株中抗菌药耐药性基因的分布情况。在目前的工作中，我们对从纳夫萨里兽医临床综合中心的犬脓皮病例中分离出的五种假中间龙线虫进行了抗菌药敏感性测试和全基因组测序。对测序数据进行了生物信息学分析，以检测 S. pseudintermedius 分离物中的抗菌药耐药性基因、质粒和移动遗传因子。所有分离出的假金黄色葡萄球菌都对阿米卡星、氨苄西林、头孢泊肟、头孢氨苄、红霉素和三甲氧苄青霉素具有耐药性。对克林霉素的敏感性较高（4/5，80%）。通过全基因组测序分析，共检测到 15 个 AMR 基因、一个质粒（repUS43）、两个插入序列（ISLgar5 和 IS256）和一个整合共轭元件（Tn6009）。这项研究有助于我们了解抗菌药耐药性与 AMR 基因、质粒和移动遗传因子之间的关系。
Profiling of antimicrobial resistance genes from Staphylococcus pseudintermedius isolated from dogs with pyoderma using whole genome sequencing.
Staphylococcus pseudintermedius is considered as a most common cause of canine pyoderma. Antimicrobial resistance is considered as a global public health concern. The main aim of the study was to evaluate the distribution of Antimicrobial resistance genes in S. pseudintermedius isolates using whole genome sequencing. In the current work, Antimicrobial susceptibility testing and Whole genome sequencing was performed on Five S. pseudintermedius isolates recovered from canine pyoderma cases presented at Veterinary Clinical Complex, Navsari. Bioinformatics analysis of sequencing data was performed to detect Antimicrobial resistance genes, plasmids and mobile genetic elements in S. pseudintermedius isolates. All S. pseudintermedius isolates shows resistance towards amikacin, ampicillin, cefpodoxime, cephalexin, erythromycin and trimethoprim. Higher sensitivity was observed against clindamycin (4/5, 80 %). A total 15 AMR genes, one plasmid (repUS43), two insertion sequences (ISLgar5 and IS256) and one Integrative Conjugative Element (Tn6009) were detected using whole genome sequencing analysis. This study aids us to know about relationship between antimicrobial resistance and presence of AMR genes, plasmids and mobile genetic elements.
期刊介绍：
Comparative Immunology, Microbiology & Infectious Diseases aims to respond to the concept of "One Medicine" and to provide a venue for scientific exchange. Based on the concept of "Comparative Medicine" interdisciplinary cooperation between specialists in human and animal medicine is of mutual interest and benefit. Therefore, there is need to combine the respective interest of physicians, veterinarians and other health professionals for comparative studies relevant to either human or animal medicine .
The journal is open to subjects of common interest related to the immunology, immunopathology, microbiology, parasitology and epidemiology of human and animal infectious diseases, especially zoonotic infections, and animal models of human infectious diseases. The role of environmental factors in disease emergence is emphasized. CIMID is mainly focusing on applied veterinary and human medicine rather than on fundamental experimental research.
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