IF 4.4 2区 医学 Q1 NEUROSCIENCES
Chuyi Su, Rosangela F Mendes-Platt, Jose-Manuel Alonso, Harvey A Swadlow, Yulia Bereshpolova
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引用次数: 0

摘要

兔 V1 第 4 层含有快速尖峰 GABA 能中间神经元(疑似抑制性中间神经元,SINs),它们接收来自 LGN 的强大突触输入,并产生快速的局部前馈抑制。这些细胞的感受野具有重叠的 ON/OFF 子区域、非线性空间总和、非常广泛的方向/方向调谐以及高自发和视觉驱动的发射率。这种快速尖峰中间神经元也存在于第 5 层(L5),该层接受的来自 LGN 的输入要稀少得多,但对 L5 SIN 的反应特性和丘脑皮层连接性的研究相对较少。在这里,我们研究了清醒兔子(雌雄均可)的 L5 SIN,并将其反应特性与之前研究过的 L4 SIN 进行了比较。我们还评估了 L5 SIN 的丘脑皮层连通性,检查了视网膜区对齐的 LGN-SIN 穗串的交叉相关性和 L5 SIN 对 LGN 电刺激的反应。这些分析证实,许多 L5 SIN 与 L4 SIN 一样,都能从 LGN 获得强大而快速的单突触驱动。此外,这些与 LGN 连接的 L5 SINs 的反应特性与 L4 SINs 相似,并且主要存在于 L5 的上半部。相比之下,对 LGN 刺激具有较长突触潜伏期的 L5 SINs 则表现出:(1)更敏锐的方向调谐;(2)更长的视觉反应潜伏期;(3)更低的自发发射率和(4)视觉驱动发射率;(5)存在于 L5 的深半部。我们认为,这种 L5 SINs 中的长延时突触反应反映了一种多突触皮层内通路,它产生的反应特性不同于直接由 LGN 输入驱动的 L5 SINs。在第 4 层,它们接收来自丘脑的强大突触输入,并产生快速的局部前馈抑制。而在第 5 层,它们被认为接受丘脑的直接输入较少,并受到皮层内输入的强烈支配。在这里,我们展示了一些第 5 层中间神经元接受来自 LGN 的强大单突触输入,与那些很少接受 LGN 直接输入的中间神经元相比,它们的视觉反应特性更类似于第 4 层中间神经元。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Fast-spike interneurons in visual cortical layer 5: Heterogeneous response properties are related to thalamocortical connectivity.

Layer 4 of rabbit V1 contains fast-spiking GABAergic interneurons (suspected inhibitory interneurons, SINs) that receive potent synaptic input from the LGN and generate fast, local feed-forward inhibition. These cells display receptive fields with overlapping ON/OFF subregions, non-linear spatial summation, very broad orientation/directional tuning, and high spontaneous and visually-driven firing rates. Such fast-spike interneurons are also found in layer 5 (L5), which receives a much sparser input from the LGN, but the response properties and thalamocortical connectivity of L5 SINs are relatively unstudied. Here, we study L5 SINs in awake rabbits (both sexes) and compare their response properties with previously studied SINs of L4. We also assess thalamocortical connectivity of L5 SINs, examining both cross-correlation of retinotopically aligned LGN-SIN spike-trains and L5 SIN responses to electrical stimulation of the LGN. These analyses confirmed that many L5 SINs, like L4 SINs, receive a strong and fast monosynaptic drive from the LGN. Moreover, these LGN-connected L5 SINs had response properties that were similar to those of L4 SINs and were predominantly found in the upper half of L5. By contrast, L5 SINs with longer synaptic latencies to LGN stimulation displayed (1) sharper orientation tuning, (2) longer visual response latencies, (3) lower spontaneous and (4) visually-driven firing rates, and (5) were found in the deeper half of L5. We suggest that the long-latency synaptic responses in such L5 SINs reflects a multi-synaptic intracortical pathway that generates a different constellation of response properties than seen in L5 SINs that are driven directly by LGN input.Significance Statement Fast-spike GABAergic interneurons are found across the entire depth of the visual cortex but may have very different response properties and functions in each cortical layer depending on a host of factors that are specific to the different layers. In layer 4, they are known to receive potent synaptic input from the thalamus and generate fast, local feed-forward inhibition. In layer 5, they are thought to receive less direct thalamic input and be strongly dominated by intracortical input. Here, we show that some layer-5 interneurons receive powerful monosynaptic input from LGN and have visual response properties more similar to layer 4 interneurons than those receiving little direct LGN input.

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来源期刊
Journal of Neuroscience
Journal of Neuroscience 医学-神经科学
CiteScore
9.30
自引率
3.80%
发文量
1164
审稿时长
12 months
期刊介绍: JNeurosci (ISSN 0270-6474) is an official journal of the Society for Neuroscience. It is published weekly by the Society, fifty weeks a year, one volume a year. JNeurosci publishes papers on a broad range of topics of general interest to those working on the nervous system. Authors now have an Open Choice option for their published articles
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