IF 9.4 1区 综合性期刊 Q1 MULTIDISCIPLINARY SCIENCES
Josefa Cruz, Enric Ureña, Luis P Iñiguez, Manuel Irimia, Xavier Franch-Marro, David Martín
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引用次数: 0

摘要

在黑腹果蝇中,成功的发育依赖于空间和时间调控轴的精确协调。时间轴通过许多基因(共同构成了蜕变基因网络)调控特定阶段的特征和发育转换。其中,蜕皮激素诱导蛋白 93F(E93)是成虫规格化的关键决定因素,但其作用机制仍不清楚。在这里,我们发现 E93 并非主要作为一种指示信号,而是通过抑制蛹分化广义因子(br)来促进成虫分化。在没有E93的情况下,蛹期持续高水平的Br会强烈抑制蛹特异性增强子,而这些增强子对翅膀的末端分化至关重要。值得注意的是,RNA-seq分析证实,蛹翅中大部分依赖于E93的转录组变化主要是由Br抑制驱动的。此外,我们还发现 Br 在幼虫和蛹前期抑制蛹增强子,以防止成虫遗传程序的过早执行,它还在幼虫发育后期抑制幼虫增强子的活性。这种作用机制似乎是双翅目昆虫的衍生特征,因为在鞘翅目昆虫Tribolium castaneum中,E93对br的抑制不足以实现成虫分化。总之,我们的研究阐明了 E93 与 Br 之间错综复杂的相互作用在果蝇末期分化过程中的关键作用。这一发现对于促进我们对昆虫变态进化的理解具有重要意义。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
E93 controls adult differentiation by repressing broad in Drosophila.

In Drosophila melanogaster, successful development relies on the precise coordination of both spatial and temporal regulatory axes. The temporal axis governs stage-specific identity and developmental transitions through a number of genes, collectively forming the Metamorphic Gene Network. Among these, Ecdysone inducible protein 93F (E93) serves as the critical determinant for adult specification, but its mechanism of action remains unclear. Here, we found that, rather than acting mainly as an instructive signal, E93 promotes adult differentiation through the repression of the pupal specifier broad (br). In the absence of E93, sustained high levels of Br during the pupal stage strongly represses pupal-specific enhancers that are essential for the terminal differentiation of the wing. Notably, RNA-seq analysis confirmed that the majority of E93-dependent transcriptomic changes in pupal wings are primarily driven by br repression. In addition, we also show that Br represses the pupal-enhancers during the larval and prepupal stages preventing the premature implementation of the adult genetic program, and that it also dampens the activity of larval enhancers during the latter stages of larval development. This mechanism of action seems to be a derived feature acquired in Diptera, as in the coleopteran Tribolium castaneum, repression of br by E93 is not sufficient to allow adult differentiation. In summary, our study elucidates the crucial role of the intricate interplay between E93 and Br as the governing mechanism in the process of terminal differentiation in Drosophila. This finding holds significant implications for advancing our understanding of the evolution of insect metamorphosis.

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来源期刊
CiteScore
19.00
自引率
0.90%
发文量
3575
审稿时长
2.5 months
期刊介绍: The Proceedings of the National Academy of Sciences (PNAS), a peer-reviewed journal of the National Academy of Sciences (NAS), serves as an authoritative source for high-impact, original research across the biological, physical, and social sciences. With a global scope, the journal welcomes submissions from researchers worldwide, making it an inclusive platform for advancing scientific knowledge.
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