Francesca Loria, Sabrina Picciotto, Giorgia Adamo, Andrea Zendrini, Samuele Raccosta, Mauro Manno, Paolo Bergese, Giovanna L. Liguori, Antonella Bongiovanni, Nataša Zarovni
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引用次数: 0
摘要
细胞外囊泡(EVs)具有细胞间交流的特性,并参与多种生物过程,因此越来越多的研究和开发将其用于不同的应用领域。然而,由于其复杂的性质和异质性,以及与其纯化和表征程序相关的挑战,需要对需要监测的定性和定量参数进行谨慎评估。这意味着任何电动汽车研究人员在研究和转化环境中都必须面对众多选择和可能的解决方案。在这方面,决策工具可以帮助评估各种选择,权衡利弊,最终做出一个深思熟虑的决定,同时考虑到最适合来源和最适合范围的电动汽车应用。在此,我们介绍一种多标准电动汽车决策网格(EV-DMG),它是一种新颖、高效、可定制且易于使用的工具,可为电动汽车研究和创新提供支持。通过识别和权衡关键评估标准,比较不同的基于电动汽车的制备和相关流程,我们的 EV-DMG 可以帮助任何电动汽车社区成员就电动汽车源或样本的管理做出知情、可追溯和可重现的决策。最终,EV-DMG 可指导采用最合适的 EV 生产和分析管道,以实现既定目标或应用。
A decision-making tool for navigating extracellular vesicle research and product development
Due to their intercellular communication properties and involvement in a wide range of biological processes, extracellular vesicles (EVs) are increasingly being studied and exploited for different applications. Nevertheless, their complex nature and heterogeneity, as well as the challenges related to their purification and characterization procedures, require a cautious assessment of the qualitative and quantitative parameters that need to be monitored. This translates into a multitude of choices and putative solutions that any EV researcher must confront in both research and translational environments. In this respect, decision-making tools may help assess various options, weigh pros and cons, and ultimately arrive at a thought-out decision that considers both the best fit-to-source and fit-to-scope EV application(s). Here, we present a multi-criteria EV decision-making grid (EV-DMG) as a novel, efficient, customizable, and easy-to-use tool to support EV research and innovation. By identifying and weighing key assessment criteria for comparing distinct EV-based preparations and related processes, our EV-DMG may assist any EV community member in making informed, traceable, and reproducible decisions regarding the management of EV sources or samples. Ultimately, this EV-DMG may guide the adoption of the most suitable EV production and analytical pipelines for targeting a defined aim or application.
期刊介绍:
The Journal of Extracellular Vesicles is an open access research publication that focuses on extracellular vesicles, including microvesicles, exosomes, ectosomes, and apoptotic bodies. It serves as the official journal of the International Society for Extracellular Vesicles and aims to facilitate the exchange of data, ideas, and information pertaining to the chemistry, biology, and applications of extracellular vesicles. The journal covers various aspects such as the cellular and molecular mechanisms of extracellular vesicles biogenesis, technological advancements in their isolation, quantification, and characterization, the role and function of extracellular vesicles in biology, stem cell-derived extracellular vesicles and their biology, as well as the application of extracellular vesicles for pharmacological, immunological, or genetic therapies.
The Journal of Extracellular Vesicles is widely recognized and indexed by numerous services, including Biological Abstracts, BIOSIS Previews, Chemical Abstracts Service (CAS), Current Contents/Life Sciences, Directory of Open Access Journals (DOAJ), Journal Citation Reports/Science Edition, Google Scholar, ProQuest Natural Science Collection, ProQuest SciTech Collection, SciTech Premium Collection, PubMed Central/PubMed, Science Citation Index Expanded, ScienceOpen, and Scopus.