松油烯-4-醇对人角膜上皮的影响。

IF 2.6 3区 医学 Q2 OPHTHALMOLOGY
Joo-Hee Park, Choul Yong Park
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引用次数: 0

摘要

目的:研究松油烯-4-醇(t40o)对原代培养人角膜上皮细胞(HCECs)的毒性。方法:将HCECs暴露于不同浓度(0% ~ 0.1%)的t40o中15分钟~ 72小时。使用CCK8试剂盒和细胞计数评估细胞活力。在t40o暴露15至60分钟后测量细胞内活性氧(ROS)。利用相衬显微镜和免疫细胞化学观察细胞形态和细胞质肌动蛋白丝的变化。Western blot检测细胞存活通路相关蛋白(mTOR、Akt、Bcl-xL、BAX)的表达水平。结果:t40诱导HCECs的剂量依赖性毒性。暴露于0.05% t40o 15分钟显著降低细胞活力。较低浓度(0.025%和0.0125%)暴露时间越长,毒性也越大。长时间暴露在t40o中会增强细胞毒性,0.05%的t40o在24小时后使细胞活力降低一半,0.1%的t40o会导致细胞完全死亡。细胞内ROS增加,磷酸化mTOR、磷酸化Akt和Bcl-xL水平降低,BAX表达增加,伴随着这种毒性。F-actin染色显示细胞粘附明显改变。结论:我们的研究表明,t40暴露会对HCECs产生显著的毒性,这取决于浓度和孵育时间。这种毒性反应与ROS增加和细胞存活途径活性降低有关。翻译相关性:本研究揭示的t40o角膜上皮毒性数据可能对未来使用茶树油或开发含茶树油的眼睑磨砂产品治疗眼睑蠕形螨感染有用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
The Effect of Terpinen-4-ol on Human Corneal Epithelium.

Purpose: To investigate the toxicity of terpinen-4-ol (T4O) on primary cultured human corneal epithelial cells (HCECs).

Methods: HCECs were exposed to various concentrations (0%-0.1%) of T4O for 15 minutes to 72 hours. Cell viability was assessed using the CCK8 kit and cell counting. Intracellular reactive oxygen species (ROS) were measured after 15 to 60 minutes of T4O exposure. Changes in cell morphology and cytoplasmic actin filaments were observed using phase contrast microscopy and immunocytochemistry. The expression levels of proteins involved in cell survival pathways (mTOR, Akt, Bcl-xL, and BAX) were evaluated by Western blot analysis.

Results: T4O induced dose-dependent toxicity in HCECs. Exposure to 0.05% T4O for 15 minutes significantly decreased cell viability. Lower concentrations (0.025% and 0.0125%) also caused significant toxicity with longer exposure times. Prolonged exposure enhanced cytotoxicity, with 0.05% T4O reducing viability by half after 24 hours and 0.1% T4O causing complete cell death. Increased intracellular ROS and decreased levels of phosphorylated mTOR, phosphorylated Akt, and Bcl-xL, along with increased BAX expression, accompanied this toxicity. F-actin staining revealed significant changes in cell adhesion.

Conclusions: Our study demonstrates that T4O exposure causes significant toxicity in HCECs, depending on concentration and incubation time. This toxic response is associated with increased ROS and decreased cell survival pathway activity.

Translational relevance: The corneal epithelial toxicity data of T4O revealed in this study may be useful in the future use of tea tree oil or the development of tee tree oil-containing eyelid scrub products for treating eyelid demodex infestation.

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来源期刊
Translational Vision Science & Technology
Translational Vision Science & Technology Engineering-Biomedical Engineering
CiteScore
5.70
自引率
3.30%
发文量
346
审稿时长
25 weeks
期刊介绍: Translational Vision Science & Technology (TVST), an official journal of the Association for Research in Vision and Ophthalmology (ARVO), an international organization whose purpose is to advance research worldwide into understanding the visual system and preventing, treating and curing its disorders, is an online, open access, peer-reviewed journal emphasizing multidisciplinary research that bridges the gap between basic research and clinical care. A highly qualified and diverse group of Associate Editors and Editorial Board Members is led by Editor-in-Chief Marco Zarbin, MD, PhD, FARVO. The journal covers a broad spectrum of work, including but not limited to: Applications of stem cell technology for regenerative medicine, Development of new animal models of human diseases, Tissue bioengineering, Chemical engineering to improve virus-based gene delivery, Nanotechnology for drug delivery, Design and synthesis of artificial extracellular matrices, Development of a true microsurgical operating environment, Refining data analysis algorithms to improve in vivo imaging technology, Results of Phase 1 clinical trials, Reverse translational ("bedside to bench") research. TVST seeks manuscripts from scientists and clinicians with diverse backgrounds ranging from basic chemistry to ophthalmic surgery that will advance or change the way we understand and/or treat vision-threatening diseases. TVST encourages the use of color, multimedia, hyperlinks, program code and other digital enhancements.
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