黄河沉积物中氨氧化剂的生态分布及其影响因素

IF 7.7 2区 环境科学与生态学 Q1 ENVIRONMENTAL SCIENCES
Xue Lou , Mengxin Xu , Mingyang Wang , Yining Jiang , Minggang Zheng , Hongyu Mu , Shuai Liu , Shaoping Kuang , Hui Chen , Zhiyao Wang
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引用次数: 0

摘要

氨氧化是硝化反应的第一步和限速步骤,是氮循环的关键步骤。氨氧化古菌(AOA)和细菌(AOB)以及完全氨氧化硝化螺旋菌(comammox)等关键氨氧化菌的分布格局为研究自然生态系统的氮循环提供了重要依据。目前,淡水生态系统中AOA、AOB和comammox Nitrospira的分布和贡献还未得到充分的研究。本研究利用高通量测序和qPCR技术对黄河沉积物中AOA、AOB和comammox Nitrospira的丰度、多样性、系统发育特征及群落结构进行了研究。与AOA和AOB相比,Comammox Nitrospira在所有采样点的沉积物中表现出最高的amoA基因丰度。AOA多样性与理化性质无显著相关,AOB多样性与pH呈负相关(p < 0.05), comammox Nitrospira多样性与NH4+含量、TC含量呈正相关(p < 0.05)。系统发育分析确定Nitrososphaera Nitrosospira, cladeA1 AOA的最具优势的集群,AOB和comammox硝化螺菌属,分别。AOA、AOB和comammox Nitrospira群落组成在上、中、下游呈现明显的空间格局。pH是影响AOA群落结构的关键因子(p < 0.05),有机碳是AOA群落结构的关键决定因素(p < 0.05)。本研究结果促进了我们对淡水生态系统氮循环的认识。
本文章由计算机程序翻译,如有差异,请以英文原文为准。

Ecological distribution of ammonia oxidizers in Yellow River sediments and their influencing factors

Ecological distribution of ammonia oxidizers in Yellow River sediments and their influencing factors
Ammonia oxidation, the first and rate-limiting step of nitrification, is a crucial step in nitrogen cycling. The distribution patterns of key ammonia oxidizers, including ammonia-oxidizing archaea (AOA) and bacteria (AOB), and comammox (complete ammonia oxidation) Nitrospira, provide vital insights for nitrogen cycling in natural ecosystems. Currently, the distribution and contribution of AOA, AOB and comammox Nitrospira in freshwater ecosystems remain largely underexplored. This study explored the abundances, diversity, phylogenetic characteristics, and community structures of AOA, AOB and comammox Nitrospira in the Yellow River sediments using high-throughput sequencing and qPCR. Comammox Nitrospira displayed the highest amoA gene abundance in sediments from all sampling sites compared to that of AOA and AOB. The diversity of AOA shown no significant correlations with physicochemical properties, while the diversity of AOB negatively correlated with pH (p < 0.05), and the diversity of comammox Nitrospira positively correlated with NH4+ content and TC content (p < 0.05), respectively. Phylogenetic analysis identified Nitrososphaera, Nitrosospira, and cladeA1 as the most dominant clusters of AOA, AOB and comammox Nitrospira, respectively. The community composition of AOA, AOB, and comammox Nitrospira exhibited distinct spatial patterns, varying across the upper, middle and lower reaches. pH was the key factor shaping the community structure of AOB and comammox Nitrospira (p < 0.05), while organic carbon was the key determinant of the AOA community structure (p < 0.05). The results of this study advance our understanding of N cycling in freshwater ecosystems.
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来源期刊
Environmental Research
Environmental Research 环境科学-公共卫生、环境卫生与职业卫生
CiteScore
12.60
自引率
8.40%
发文量
2480
审稿时长
4.7 months
期刊介绍: The Environmental Research journal presents a broad range of interdisciplinary research, focused on addressing worldwide environmental concerns and featuring innovative findings. Our publication strives to explore relevant anthropogenic issues across various environmental sectors, showcasing practical applications in real-life settings.
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