Yudai Izumi, Ralph Ugalino, Jun Miyawaki, Chie Shibazaki, Motoyasu Adachi, Naoya Kurahashi, Hisao Kiuchi, Yoshihisa Harada and Kentaro Fujii
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Electronic structures of blue copper centers of amicyanin and azurin in the solution state†
X-ray absorption near edge structure (XANES) spectra of blue copper proteins, amicyanin and azurin, in the solution state were measured in the copper L3-edge energy region. The absorption peak energies were quite similar for both proteins, while the main edge region for azurin was broader than that for amicyanin, owing to more pronounced shoulder spectral features in the former. Ab initio calculations at the whole protein level qualitatively reproduced the experimental spectra well. The relative X-ray absorption intensities suggest that the degree of covalency of the copper–ligand bond at the active site was weaker for amicyanin than that for azurin.
期刊介绍:
Dalton Transactions is a journal for all areas of inorganic chemistry, which encompasses the organometallic, bioinorganic and materials chemistry of the elements, with applications including synthesis, catalysis, energy conversion/storage, electrical devices and medicine. Dalton Transactions welcomes high-quality, original submissions in all of these areas and more, where the advancement of knowledge in inorganic chemistry is significant.