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引用次数: 0
摘要
背景：本研究的目的是评估阿斯巴甜对BALB/c小鼠的潜在免疫毒性作用。方法：阿斯巴甜400、2000 mg/kg口服2周（每周5天）。评估体液和细胞免疫反应的具体参数，包括血凝抗体（HA）滴度、细胞因子产生（IFN-γ和IL-4水平）、对srbc的延迟型超敏反应（DTH）、脾脏和骨髓的组织病理学检查以及t淋巴细胞对植物血凝素- a （PHA）的反应。结果与结论：400和2000 mg/kg阿斯巴甜对大鼠血液学和组织病理学指标、血凝素滴度、IFN-γ和IL-4水平、DTH和淋巴细胞增殖反应无显著影响（p > 0.05）。400mg /kg和2000mg /kg阿斯巴甜对小鼠免疫系统参数无明显影响。阿斯巴甜对BALB/c小鼠免疫系统是安全的。
Aspartame subacute exposure does not affect immune system of BALB/c mice following a tiered approach.
Background: The objective of the present study was to assess potential immunotoxic effects of aspartame in BALB/c mice.
Methods: Aspartame was administered orally at 400 and 2000 mg/kg for two weeks (five days per week). Specific parameters of humoral and cellular immune responses including hemagglutinating antibody (HA) titer, cytokine production (IFN-γ and IL-4 levels), delayed type hypersensitivity (DTH) response to SRBCs, histopathological examination of spleen and bone marrow, and T-lymphocyte proliferation in response to phytohemagglutinin-A (PHA) were evaluated.
Results and conclusion: Aspartame at 400 and 2000 mg/kg did not significantly change hematological and histopathological parameters, HA titer, IFN-γ and IL-4 levels, DTH, and lymphoproliferation responses (p > 0.05). Aspartame at 400 and 2000 mg/kg did not induce any noticeable effects in immune system parameters of mice after a 14-day feeding. Aspartame was found to be safe to BALB/c mice immune system.
期刊介绍：
Tissue and Cell is devoted to original research on the organization of cells, subcellular and extracellular components at all levels, including the grouping and interrelations of cells in tissues and organs. The journal encourages submission of ultrastructural studies that provide novel insights into structure, function and physiology of cells and tissues, in health and disease. Bioengineering and stem cells studies focused on the description of morphological and/or histological data are also welcomed.
Studies investigating the effect of compounds and/or substances on structure of cells and tissues are generally outside the scope of this journal. For consideration, studies should contain a clear rationale on the use of (a) given substance(s), have a compelling morphological and structural focus and present novel incremental findings from previous literature.
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