脂质重组酶TMEM41B参与gpi锚定蛋白的加工和运输。

IF 2.1 4区 生物学 Q4 BIOCHEMISTRY & MOLECULAR BIOLOGY
Hiroto Hirayama
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引用次数: 0

摘要

糖基磷脂酰肌醇(GPI)锚定是真核生物中保守的翻译后修饰。这种修饰允许受体蛋白在细胞表面以gpi锚定蛋白(GPI-APs)的形式表达,在各种生物过程中发挥关键作用。有研究提出,受体蛋白转移后GPI的重塑,包括GPI肌醇的pgap1依赖性去酰化,作为将成熟GPI- ap从内质网转运到高尔基体的检查点。先前的一项研究发现了几个参与调节pgap1依赖性gpi -肌醇去酰化的因素,包括与钙连联蛋白周期、SELT和CLPTM1相关的蛋白质。Cao等人最近的一篇报道显示,在SELT-KO细胞中,TMEM41B(一种内质酶)的缺失可以修复gpi -肌醇去酰化的缺陷。进一步的研究表明,TMEM41B对于GPI-APs和跨膜蛋白从内质网到高尔基体的有效运输至关重要。研究还发现,PGAP1蛋白在TMEM41B-KO细胞内质网中积累,提示内质网膜脂质完整性的扰动稳定了PGAP1蛋白,从而增强了内质网内PGAP1的活性。这些发现强调了TMEM41B的缺陷影响两个不同的过程:(i) gpi - ap从内质网到高尔基体的运输,以及(ii) gpi - ap的去酰化。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Commentary for: a lipid scramblase TMEM41B is involved in the processing and transport of GPI-anchored proteins.

Glycosylphosphatidylinositol (GPI) anchoring is a conserved post-translational modification in eukaryotes. This modification allows acceptor proteins to be expressed at the cell surface as GPI-anchored proteins (GPI-APs), which play critical roles in various biological processes. It has been proposed that remodelling of GPI after transferring acceptor proteins, including the PGAP1-dependent deacylation of GPI-inositol, functions as a checkpoint for transporting mature GPI-APs from the endoplasmic reticulum (ER) to the Golgi. A previous study identified several factors involved in regulating PGAP1-dependent GPI-inositol deacylation, including proteins associated with the calnexin cycles, SELT and CLPTM1. A recent report by Cao et al., revealed that the loss of TMEM41B, an ER-resident lipid scramblase, rescues the defect in GPI-inositol deacylation in SELT-KO cells. Further investigation demonstrated that TMEM41B is essential for the efficient transport of both GPI-APs and transmembrane proteins from the ER to the Golgi. The study also found that PGAP1 proteins accumulate in the ER of TMEM41B-KO cells, suggesting that perturbations in the ER-membrane lipid integrity stabilize PGAP1 proteins, thereby enhancing the PGAP1 activity within the ER. These findings highlight that defects in TMEM41B impact two distinct processes: (i) the transport of GPI-APs from the ER to the Golgi, and (ii) the deacylation of GPI-APs.

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来源期刊
Journal of biochemistry
Journal of biochemistry 生物-生化与分子生物学
CiteScore
4.80
自引率
3.70%
发文量
101
审稿时长
4-8 weeks
期刊介绍: The Journal of Biochemistry founded in 1922 publishes the results of original research in the fields of Biochemistry, Molecular Biology, Cell, and Biotechnology written in English in the form of Regular Papers or Rapid Communications. A Rapid Communication is not a preliminary note, but it is, though brief, a complete and final publication. The materials described in Rapid Communications should not be included in a later paper. The Journal also publishes short reviews (JB Review) and papers solicited by the Editorial Board.
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